Modulation of Graft-versus-Host (GvH) Disease in the Rat; Effect of Hydroxyurea on the Mixed Lymphocyte Reaction and Graft-versus-Host Reactivity
The graft-versus-host (GvH) reaction is a complex biologic phenomenon, the underlying cellular events of which are not fully defined (for reviews, see refs. 8 and 14). Studies in congenic mice indicate an important relationship between the Ir region of the H-2 gene complex and GvH reactivity (9, 18, 20). Because Ir gene products are known to influence lymphocyte recognition and proliferation in the mixed lymphocyte reaction (MLR) (1, 2, 19), it has been suggested that GvH and MLR genes are identical (18, 25). There are considerable data to support this concept as well as data to suggest an important role for non-MLR and non-Ir region genes in initiating GvH disease. Isolated K-region and possibly D-region differences can produce a weak GvH reaction (18) as can non-H-2 loci (5, 10). Demonstration of GvH reactivity of non-Ir region loci depends on the assay employed and frequently occurs in the absence of a detectable MLR or the generation of cytotoxic lymphocytes. GvH disease frequently develops in dogs and primates grafted with MLR nonreactive allogeneic bone marrow (7, 27, 28) without acquisition of MLR reactivity (22). Finally, there are preliminary data, which suggest that GvH and MLR reactive cells represent separate T-lymphocyte subpopulations expressing quantitative differences in Thy-1 antigen and in Ly alleles (15, 17).
KeywordsCellulose Agar Cage Leukemia Penicillin
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