Recognition of Hemopoietic Progenitors

  • J. E. Till
Conference paper
Part of the Experimental Hematology Today book series (HEMATOLOGY, volume 1977)

Abstract

The revolution in experimental hematology brought about by the introduction of colony assays for hemopoietic progenitor cells is still running its course. Colony assays have now been described for pluripotent stem cells (20), granulocyte and macrophage progenitors (3, 10, 13, 16), erythropoietic progenitors (1, 18), megakaryocyte precursors (12, 15), B- and T-lymphocyte precursors (5, 14, 17), and cells in leukemic marrow (4). For all the above cell types except pluripotent stem cells, the assays involve colony formation in cell culture. There is scope for more advances along these lines as culture methods are refined further, particularly in regard to the development of a culture assay for pluripotent stem cells.

Keywords

Sedimentation Erythropoietin Mast 

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References

  1. (1).
    Axelrad, A. A., McLeod, D. L., Shreeve, M. M., and Heath, D. Properties of cells that produce eryth-rocytic colonies in vitro. In Robinson, W. A., ed., Hemopoiesis in Culture: Second International Workshop. Washington, D.C.: U.S. Government Printing Office, 1974, p. 226.Google Scholar
  2. (2).
    Bonner, W. A., Hulett, H. R., Sweet, R. G., and Herzenberg, L. A. Fluorescence activated cell sorting. Rev. Sci. Instrum., 43: 404, 1972.PubMedCrossRefGoogle Scholar
  3. (3).
    Bradley, T. R., and Metcalf, D. The growth of mouse bone marrow cells in vitro. Aust. J. Exp. Biol Med. Sci., 44: 287, 1966.PubMedCrossRefGoogle Scholar
  4. (4).
    Dicke, K. A., Spitzer, G., and Ahearn, M. J. Colony formation in vitro by leukaemic cells in acute myelogenous leukaemia with phytohaem-agglutinin as stimulating factor. Nature (London), 259: 129, 1976.CrossRefGoogle Scholar
  5. (5).
    Fibach, E., Gerassi, E., and Sachs, L. Induction of colony formation in vitro by human lymphocytes. Nature (London), 259: 127, 1976.CrossRefGoogle Scholar
  6. (6).
    Gregory, C. J. Erythropoietin sensitivity as a differentiation marker in the hemopoietic system: Studies of three erythropoietic colony responses in culture. J. Cell. Physiol, (in press).Google Scholar
  7. (7).
    Gregory, C. J., McCulloch, E. A., and Till, J. E. Erythropoietic progenitors capable of colony formation in culture: State of differentiation. J. Cell. Physiol, 81: 411, 1973.PubMedCrossRefGoogle Scholar
  8. (8).
    Heath, D. S., Axelrad, A. A., McLeod, D. L., and Shreeve, M. M. Separation of the erythropoietin responsive progenitors BFU-E and CFU-E in mouse bone marrow by unit gravity sedimentation. Blood, 47: 777, 1976.PubMedGoogle Scholar
  9. (9).
    Korn, A. P., Henkelman, R. M., Ottensmeyer, F. P., and Till, J. E. Investigations of a stochastic model of haemopoiesis. Exp. Hematol. 1: 362, 1973.PubMedGoogle Scholar
  10. (10).
    Lin, H. S., and Stewart, C. C. Colony formation by mouse peritoneal exudate cells in vitro. Nature (London), New Biol. 243: 176, 1973.Google Scholar
  11. (11).
    McCulloch, E. A. Les clones de cellules hématopoiétiques in vivo. Rev. Fr. Etud. Clin. Biol, 8: 15, 1963.Google Scholar
  12. (12).
    Metcalf, D., MacDonald, H. R., Odartchenko, N., and Sordat, B, Growth of mouse megakaryocyte colonies in vitro. Proc. Nat. Acad. Sci. U.S.A., 12: 1744, 1975.CrossRefGoogle Scholar
  13. (13).
    Metcalf, D., Parker, J., Chester, H. M., and Kincade, P. W. Formation of eosinophilic-like granulocy¬tic colonies by mouse bone marrow cells in vitro. J. Cell Physiol, 84: 275, 1974.PubMedCrossRefGoogle Scholar
  14. (14).
    Metcalf, D., Warner, N. L., Nossal, G. J. V., Miller, J. F. A. P., Shortman, K., and Rabellino, E. Growth of B lymphocyte colonies in vitro from lymphoid organs. Nature (London), 255: 630, 1975.CrossRefGoogle Scholar
  15. (15).
    Nakeff, A., and Daniels-McQueen, S. In vitro colony assay for a new class of megakaryocyte precursor: Colony-forming unit megakaryocyte (CFU-M). Proc. Soc. Exp. Biol Med., 151: 587, 1976.Google Scholar
  16. (16).
    Pluznik, D., and Sachs, L. The cloning of normal “mast” cells in tissue culture. J. Cell Comp. Physiol, 66: 319, 1965.CrossRefGoogle Scholar
  17. (17).
    Sredni, B., Kalechman, Y., Michlin, H., and Rozenszajn, L. A. Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes. Nature (London), 259: 130, 1976.CrossRefGoogle Scholar
  18. (18).
    Stephenson, J. R., Axelrad, A. A., McLeod, D. L., and Shreeve, M. M. Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc. Nat. Acad. Sci. U.S.A., 68: 1542, 1971.CrossRefGoogle Scholar
  19. (19).
    Till, J. E. Regulation of hemopoietic stem cells. In Cairnie, A. B., Lala, P. K., and Osmond, D. G., eds., Stem Cells of Renewing Cell Populations. New York: Academic Press, 1976, p. 143.Google Scholar
  20. (20).
    Till, J. E., and McCulloch, E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat. Res., 14: 213, 1961.PubMedCrossRefGoogle Scholar
  21. (21).
    Till, J. E., McCulloch, E. A., and Siminovitch, L. A stochastic model of stem cell proliferation, based on the growth of spleen colony-forming cells. Proc. Nat. Acad. Sci. U.S.A., 51: 29, 1964.CrossRefGoogle Scholar
  22. (22).
    Trentin, J. J. Hemopoietic inductive microenvironments. In Cairnie, A. B., Lala, P. K., and Osmond, D. G., eds., Stem Cells of Renewing Cell Populations. New York: Academic Press, 1976, p. 255Google Scholar
  23. (23).
    Wu, A. M., Siminovitch, L., Till, J. E., and McCulloch, E. A. Evidence for a relationship between mouse hemopoietic stem cells and cells forming colonies in culture. Proc. Nat. Acad. Sci. U.S.A., 59: 1209, 1968.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

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  • J. E. Till

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