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A Peptide Inhibitor of Glucagon-Responsive Adenylate Cyclase in Liver

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GLUCAGON: Its Role in Physiology and Clinical Medicine

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Abstract

The effects of a peptide inhibitor of adenylate cyclase produced by the isolated, perfused rat liver under hypocalcemic conditions were studied. This inhibitory peptide non-competitively abolished the activation of adenylate cyclase in particulate preparations of rat liver by glucagon, epinephrine, and parathyroid hormone, as well as cyclic AMP production in glucagon-stimulated rat liver slices. Higher concentrations of inhibitor also decreased basal adenylate cyclase activity and its fluoride-responsiveness. The data suggest that this substance is normally present in liver, but is released only under hypocalcemic conditions. The peptide does not crossreact in the radioimmunoassay of glucagon, insulin, or parathyroid hormone. Its inhibitory effects are not duplicated by somatostatin, angiotensin I, renin substrate, or the desoctadecapeptide of insulin.

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References

  1. Gilman, A. G. (1970): A protein binding assay for adenosine S-cyclic monophosphate. Proc. Nat. Acad. Sci. USA 67, 305–312.

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  5. Levey, G. S., Lehotay, D. C., Canterbury, J. M., Bricker, L. A. and Meltz, G. J. (1975): Isolation of a unique peptide inhibitor of hormone-responsive adenylate cyclase. J. Biol. Chem. 250, 5730–5733.

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Authors and Affiliations

  1. Division of Endocrinology and Metabolism, Department of Medicine, University of Miami School of Medicine, Miami, Florida, USA

    D. C. Lehotay, G. S. Levey, J. M. Canterbury, L. A. Bricker & E. Ruiz

Authors
  1. D. C. Lehotay
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  2. G. S. Levey
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  3. J. M. Canterbury
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  4. L. A. Bricker
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  5. E. Ruiz
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Editor information

Editors and Affiliations

  1. Department of Physiology, Wayne State University School of Medicine, 48201, Detroit, Michigan, USA

    Piero P. Foà

  2. Department of Medicine, All-India Institute of Medical Sciences, 110016, New Delhi, India

    Jasbir S. Bajaj

  3. Department of Research, Sinai Hospital of Detroit, 48235, Detroit, Michigan, USA

    Naomi L. Foà

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© 1977 Springer-Verlag New York Inc.

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Lehotay, D.C., Levey, G.S., Canterbury, J.M., Bricker, L.A., Ruiz, E. (1977). A Peptide Inhibitor of Glucagon-Responsive Adenylate Cyclase in Liver. In: Foà, P.P., Bajaj, J.S., Foà, N.L. (eds) GLUCAGON: Its Role in Physiology and Clinical Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6366-1_25

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  • DOI: https://doi.org/10.1007/978-1-4612-6366-1_25

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-6368-5

  • Online ISBN: 978-1-4612-6366-1

  • eBook Packages: Springer Book Archive

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