Colony-forming Cells in the Thymus and Mesenteric Lymph Nodes of Mice Engrafted with Lewis Lung Carcinoma Cells

  • G. D. Ledney
  • T. J. MacVittie
  • D. A. Stewart
  • G. A. Parker
Conference paper
Part of the Experimental Hematology Today book series (HEMATOLOGY, volume 1978)


The in vitro culture of granulocyte-macrophage progenitor cells from a number of anatomical sites of the mouse apparently results in the clonal growth of at least two subpopulations of cells. One subpopulation, currently designated colony-forming cells (CFC), has been detected in the antigen-stimulated pleural and peritoneal cavities (4,10,11) and in the blood (9). These cells are also found in the lymph nodes, thymus, spleen, and bone marrow of the mouse (12,13,14). The other subpopulation of cells exhibiting in vitro clonal potential is designated the colony-forming unit culture (CFU-c), which are detected in cultures of adult mouse bone marrow, spleen, and peripheral blood. In addition to differing in their tissue origin, CFC differ from CFU-c in a number of other ways. The CFC have a 10- to 15-day lag period prior to the initiation of colony formation, which reaches maximum numbers 25 days after in vitro culture. These cells develop along the monocyte-macrophage cell line and have a marked ability to survive in culture in the absence of pregnant mouse uterine extract (PMUE).


Mesenteric Lymph Node Clonal Growth Normal Human Serum Lewis Lung Carcinoma Cell Mouse Bone Marrow Cell 
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  1. 1.
    Baum, M., and Fisher, B., Macrophage production by the bone marrow of tumor-bearing mice. Cancer Res., 23: 2813, 1972.Google Scholar
  2. 2.
    Bradley, T.R., Stanley, E.R., and Sumner, M.A., Factors from mouse tissue stimulating colony growth of mouse bone marrow cells in vitro. Aust. J. Exp. Biol. Med. Sci., 49: 595, 1971.PubMedCrossRefGoogle Scholar
  3. 3.
    Bradley, T.R., Telfer, P.A., and Fry, P., The effect of erythrocytes on mouse bone marrow colony development in vitro. Blood, 38: 353, 1971.PubMedGoogle Scholar
  4. 4.
    Chu, J-Y., and Lin, H., Induction of macrophage colony-forming cells in the pleural cavity. J. Reticuloendothel. Soc., 20: 299, 1976.PubMedGoogle Scholar
  5. 5.
    Fauve, R.M., Hevin, B., Jacob, H., Gaillard, J.A., and Jacob, F., Antiinflammatory effects of murine malignant cells. Proc. Nat. Acad. Sci. (USA) 71: 4052, 1974.CrossRefGoogle Scholar
  6. 6.
    Hibberd, A.H., and Metcalf, D., Proliferation of macrophage and granulocyte precursors in response to primary and transplanted tumors. In D.W. Weiss, ed., Immunological Parameters of Host-Tumor Relationships. New York: Academic Press, pp. 202–210.Google Scholar
  7. 7.
    Ledney, G.D., Moniot, J.V., Gambrill, M.R., and MacVittie, T.J., Mitogenic and colony forming unit responses of spleen cells from mice engrafted with Lewis lung (3LL) carcinoma cells. Exp. Hematol., 4 (Suppl.): 189, 1976.Google Scholar
  8. 8.
    Levy, M.H., and Wheelock, E.F., The role of macrophages in defense against neoplastic disease. Adv. Cancer Res., 20: 131, 1974.PubMedCrossRefGoogle Scholar
  9. 9.
    Lin, H., Colony formation in vitro by mouse blood monocytes. Blood, 49: 593, 1977.PubMedGoogle Scholar
  10. 10.
    Lin, H., and Stewart, C.C., Colony formation by mouse peritoneal exudate cells in vitro. Nature (Lond), 243: 116, 1973.Google Scholar
  11. 11.
    Lin, H., and Stewart, C.C., Peritoneal exudate cells, I. Growth requirement of cells capable of forming colonies in soft agar. J. Cell. Physiol., 83: 369, 1974.PubMedCrossRefGoogle Scholar
  12. 12.
    MacVittie, T.J., and McCarthy, K.F., The detection of in vitro monocyte-macrophage colony-forming cells in mouse thymus and lymph nodes. J. Cell. Physiol., 92: 203, 1977.PubMedCrossRefGoogle Scholar
  13. 13.
    MacVittie, T.J., and Provaznik, M., The detection of in vitro monocyte macrophage colony-forming cells in mouse marrow, spleen, and peripheral blood. Exp. Hematol., 5 (Suppl. 2): 28, 1977.Google Scholar
  14. 14.
    MacVittie, T.J., and Weatherly, T.L., Characteristics of the in vitro monocyte-macrophage colony-forming cells detected within mouse thymus and lymph nodes. In S.J. Baum and G.D. Ledney, eds., Experimental Hematology Today. New York: Springer-Verlag: 1977, pp. 147–156.Google Scholar
  15. 15.
    Metcalf, D., MacDonald, H.R., and Chester, H.M., Serum potentiation of granulocyte and macrophage colony formation in vitro. Exp. Hematol., 3: 261, 1975.PubMedGoogle Scholar
  16. 16.
    Milas, L., and Tomljanovic, M., Spleen colony-forming capacity of bone marrow from mice bearing fibrosarcoma. Rev. Europ. Etudes Clin. Biol. 16: 462, 1971.Google Scholar
  17. 17.
    Nooter, K., and Bentvelzen, P., Cell density-dependent growth in agar of bone marrow cells from tumor-bearing BALB/c mice in the absence of a colony-stimulating factor. Cancer Res., 55: 117, 1975.Google Scholar
  18. 18.
    Sheil, J.M., Crawford, R.M., and Ledney, G.D., Peritoneal macrophage chemotactic response and accumulation in mice injected with Lewis lung (3LL) carcinoma cells. Exp. Hematol., 4 (Suppl.): 127, 1976.Google Scholar
  19. 19.
    Siegel, S., Nonparametric Statistics. New York: McGraw-Hill, 1956, 312 p.Google Scholar
  20. 20.
    Snodgrass, M.J., Morahan, P.S., and Kaplan, A.M., Histopathology of the host response to Lewis lung carcinoma: Modulation by Pyran. J. Natl. Cancer Inst., 55: 455, 1975.PubMedGoogle Scholar
  21. 21.
    Treves, A.J., Cohen, I.R., and Feldman, M., A syngeneic metastatic tumor model in mice: The natural immune response of the host and its manipulation. Israel J. Med. Sci., 72: 369, 1976.Google Scholar
  22. 22.
    Weatherly, T.L., Enhancement of in vitro colony formation by human serum. Exp. Hematol., 4 (Suppl.): 35, 1975.Google Scholar
  23. 23.
    Williams, N., and van den Engh, G.J., Separation of subpopulations of in vitro colony-forming cells from mouse marrow by equilibrium density centrifugation. J. Cell. Physiol., 86: 237, 1975.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1978

Authors and Affiliations

  • G. D. Ledney
  • T. J. MacVittie
  • D. A. Stewart
  • G. A. Parker

There are no affiliations available

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