Colony-forming Cells in the Thymus and Mesenteric Lymph Nodes of Mice Engrafted with Lewis Lung Carcinoma Cells
The in vitro culture of granulocyte-macrophage progenitor cells from a number of anatomical sites of the mouse apparently results in the clonal growth of at least two subpopulations of cells. One subpopulation, currently designated colony-forming cells (CFC), has been detected in the antigen-stimulated pleural and peritoneal cavities (4,10,11) and in the blood (9). These cells are also found in the lymph nodes, thymus, spleen, and bone marrow of the mouse (12,13,14). The other subpopulation of cells exhibiting in vitro clonal potential is designated the colony-forming unit culture (CFU-c), which are detected in cultures of adult mouse bone marrow, spleen, and peripheral blood. In addition to differing in their tissue origin, CFC differ from CFU-c in a number of other ways. The CFC have a 10- to 15-day lag period prior to the initiation of colony formation, which reaches maximum numbers 25 days after in vitro culture. These cells develop along the monocyte-macrophage cell line and have a marked ability to survive in culture in the absence of pregnant mouse uterine extract (PMUE).
KeywordsMesenteric Lymph Node Clonal Growth Normal Human Serum Lewis Lung Carcinoma Cell Mouse Bone Marrow Cell
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