Physical Separation and Kinetics of Colony-forming Cells in Diffusion Chambers in Vivo (CFU-d) and Colony-forming Cells in Agar in Vitro (CFU-c)
Normal human bone marrow contains cells capable of forming hemopoietic colonies in fibrin clot diffusion chambers implanted into the peritoneum (i.p.) of sublethally irradiated mice (2,5,9,10). Such colony-forming units in diffusion chambers (CFU-d) give rise to neutrophilic, eosinophilic, megakaryocytic, and fibroblast-like colonies, which are usually scored after 14 days of culture (9). The formation of neutrophilic colonies is stimulated when the host mice are irradiated (450 to 600 R) prior to chamber implantation, and the chambers are reimplanted into other irradiated mice after 7 days of i.p. culture (9). This enhancement is due to an effect on precursor cells, which initiate DNA synthesis in cultures in irradiated mice but do not synthesize DNA in non-irradiated mice; they are apparently influenced by diffusible, species non-specific stimulatory or inhibitory host factors (6).
KeywordsAlbumin Agar Sedimentation Thrombin Fibrinogen
Unable to display preview. Download preview PDF.
- 1.Broxmeyer, H.E., Baker, F.L., and Galbraith, P.R., In vitro regulation of granulopoiesis in human leukemia: Application of an assay for colony inhibiting cells. Blood, 47: 389, 1976.Google Scholar
- 2.Elmgreen, J., Jacobsen, N., and Knudtzon, S., The effect of syngeneic peripheral blood cells on the formation of colonies by normal human bone marrow cells in diffusion chambers in mice. Scand. J. Haematol., 17: 319, 1976.Google Scholar
- 4.Iscove, N.N., Messner, H., Till, J.E., and McCulloch, E.A., Human marrow cells forming colonies in culture: Analysis by velocity sedimentation and suspension culture. Ser. Haematol., 2: 31, 1972.Google Scholar
- 7.Jacobsen, N., Broxmeyer, H.E., Grossbard, E., and Moore, M.A.S., Diversity of human granulopoietic precursor cells: Separation of cells that form colonies in diffusion chambers (CFU-d) from populations of colony-forming cells in vitro (CFU-c) by velocity sedimentation. Submitted for publicationGoogle Scholar
- 8.Jacobsen, N., Broxmeyer, H.E., Grossbard, E., and Moore, M.A.S., In manuscriptGoogle Scholar
- 10.Jacobsen, N., and Fauerholdt, L., Quantitative aspects of an in vivo diffusion chamber assay for normal human hemopoietic colony-forming units. Exp. Haematol., 5: 171, 1977.Google Scholar
- 12.Robinson, W.A., and Pike, B.L., Colony growth of human bone marrow cells in vitro. In F. Stohlman, ed., Hemopoietic Cellular Proliferation. New York and London: Grune and Stratton, 1970, p. 249.Google Scholar
- 13.Sutherland, D.J.A., Till, J.E., and McCulloch, E.A., Short-term cultures of mouse marrow cells separated by velocity sedimentation. Cell Tissue Kinet, 4: 419, 1971.Google Scholar