Histocompatibility Testing for Bone-marrow Grafting

  • M. Jeannet
  • B. Speck
Conference paper
Part of the Experimental Hematology Today book series (HEMATOLOGY, volume 1978)


At the present time, the three main indications for bone marrow transplantation are severe combined immunodeficiency (SCID), bone marrow aplasia, and acute leukemia. In these diseases, the success of bone marrow transplantation is hampered by many difficulties. These difficulties include the lack of a suitable compatible donor, the frequent development of graft-versus-host disease (GvHD), and the occurrence of graft rejection. The latter is mainly seen in patients with severe aplastic anemia (SAA). Despite considerable recent advances in our knowledge of the major histocompatibility complex (MHC), and in the use of HLA-identical sibling donors for marrow transplantation, the problems of GvHD and graft rejection remain unsolved.


Aplastic Anemia Graft Rejection Severe Aplastic Anemia Mixed Lymphocyte Culture Marrow Graft 
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  1. 1.
    Bondevik, H., and Thorsby, E., Still a role for HLA in the MLC interaction. In Lymphocyte Recognition and Effector Mechanisms. New York, London: Academic Press, 1974, p. 225.Google Scholar
  2. 2.
    Colombani, J., and Colombani, M., Serologic recognition of histocompatibility antigens using complement fixation. Semin. Hematol., 2: 273, 1974.Google Scholar
  3. 3.
    Dupont, B., Hansen, J.A., Good, R.A., and O’Reilly, R., Histocompatibility testing for clinical bone marrow transplantation. In Ferrara, G.B., ed., HLA System — New Aspects. Amsterdam: Elsevier/North Holland Biomedical Press, 1977, p. 153.Google Scholar
  4. 4.
    Feig, S.A., Opelz, G., Winter, H.S., Falk, P.M., Neerhout, R.C., Sparkes, R., and Gale, R.P., Successful bone-marrow transplantation against mixed lymphocyte culture barrier. Blood, 48: 385, 1975.Google Scholar
  5. 5.
    Gatti, R.F., Meuwissen, H.J., Terasaki, P.I., and Good, R.A., Recombination within the HLA-A locus. Tissue Antigens, 7: 239, 1971.Google Scholar
  6. 6.
    Gluckman, E., Andersen, E., Lepage, U., and Dausset, J., Non-HLA lymphocytotoxic antibodies during GVHD after bone marrow transplantation (BMT). Transpl. Proc., 11: 761, 1977.Google Scholar
  7. 7.
    Goldmann, S.F., Selection of related and unrelated bone marrow donors. Transplant. Proc., 9: 507, 1977.PubMedGoogle Scholar
  8. 8.
    Goulmy, E., Termijtelen, A., Bradley, B.A., and Van Rood, J.J., Y-antigen killing by T cells of women is restricted by HLA. Nature (Lond), 266: 544, 1977.CrossRefGoogle Scholar
  9. 9.
    Hansen, J.A., Good, R.A., and Dupont, B., HLA-D compatibility between parent and child. Transplantation, 23: 366, 1977.PubMedCrossRefGoogle Scholar
  10. 10.
    Jeannet, M., Klouda, P.T., Vassalli, P., Ramirez, E., Legendre, C., and Speck, B., Anomalous MLC and CML Tests in Human Bone Marrow Transplantation in Histocompatibility Testing. Copenhagen: Munksgaard, 1975, p. 885.Google Scholar
  11. 11.
    Jeannet, M., Pinn, V.W., Flax, M.H., Winn, H.J., and Russell, P.S., Humoral antibodies in renal allotransplantation in man. N. Eng. J. Med., 3: 282, 1970.Google Scholar
  12. 12.
    Jeannet, M., Rubinstein, A., and Pelet, B., Studies on non-HLA cytotoxic and blocking factor in a patient with immunological deficiency successfully reconstituted by bone-marrow transplantation. Tissue Antigens, 5: 411, 1973.Google Scholar
  13. 13.
    Jeannet, M., Rubinstein, A., Pelet, B., and Kummer, H., Prolonged remission of severe aplastic anemia after ALG pretreatment and HLA semi-incompatible bone-marrow cell transfusion. Transplant. Proc., 6: 359, 1974.PubMedGoogle Scholar
  14. 14.
    Jeannet, M., and Vassalli, P., The role of lymphocyte dependent antibody in kidney transplantation. Transplantation, 22: 493, 1976.PubMedCrossRefGoogle Scholar
  15. 15.
    Klein, J., Relative importance of H2-regions in the development of graft-versus-host reactions. Transplant. Proc., 8: 335, 1975.Google Scholar
  16. 16.
    Klouda, P.T., and Jeannet, M., Cold and warm antibodies and graft survival in kidney allograft recipients. Lancet, i: 876, 1976.Google Scholar
  17. 17.
    Lalezari, P., and Rädel, E., Neutrophil-specific antigens: Immunology and clinical significance. Semin. Hematol., 2: 281, 1974.Google Scholar
  18. 18.
    Mathe, G., Amiel, J.H., Schwarzenberg, H., Choay, J., Trolard, P., Hayar, M., Schlumberger, J.R., and Jasmin, C., Bone-marrow graft in man after conditioning by antilymphocytic serum. Transplant. Proc., 3: 325, 1971.PubMedGoogle Scholar
  19. 19.
    Mickelson, E.M., Fefer, A., Storb, R., and Thomas, E.D., Correlation of the relative response index with marrow graft rejection in patients with aplastic anemia. Transplantation, 22: 294, 1976.PubMedCrossRefGoogle Scholar
  20. 20.
    Opelz, G., Territo, M.C., Gale, R.P., and Sparkes, R., Sensitization against non-HLA antigens following bone marrow graft rejection. Tissue Antigens, 9: 209, 1977.PubMedCrossRefGoogle Scholar
  21. 21.
    Parkman, R., Rosen, F.S., Rappeport, J., Camitta, B., Levey, R.L., and Nathan, D.G., Detection of genetically determined histocompatibility antigen differences between HLA identical and MLC non-re-active siblings. Transplantation, 27: 110, 1976.CrossRefGoogle Scholar
  22. 22.
    Speck, B., Cornu, P., Jeannet, M., Nissen, C., Burri, H.P., Groff, P., Nagel, G.A., and Buckner, C.D., Autologous marrow recovery following allogeneic marrow transplantation in a patient with severe aplastic anemia. Exp. Hematol., 4: 131, 1976.PubMedGoogle Scholar
  23. 23.
    Speck, B., Cornu, P., Sartorius, J., Nissen, C., Groff, G., Burri, H.P., and Jeannet, M., Immunologic aspects of aplasia. Transplant. Proc. (in press).Google Scholar
  24. 24.
    Speck, B., Zwaan, F.E., Van Rood, J.J., and Eernisse, J.G. Allogeneic bone-marrow transplantation in a patient with aplastic anemia using a phenotypically HLA-identical unrelated donor. Transplantation, 16: 24, 1973.PubMedCrossRefGoogle Scholar
  25. 25.
    Storb, R., Prentice, R.L., and Thomas, E.D., Treatment of aplastic anemia by marrow transplantation from HLA identical siblings. Prognostic factors associated with graft—versus—host disease and survival. J. Clin. Invest., 9: 625, 1977.CrossRefGoogle Scholar
  26. 26.
    Storb, R., Thomas, E.D., Weiden, P.L., Buckner, C. D., Clift, R.A., Fefer, A., Fernando, L.P., Giblett, E.R., Goodell, B.W., Johnson, F.L., Lerner, K.G., Neiman, P.E., and Sanders, J.E., Aplastic anemia treated by allogeneic bone-marrow transplantation: a report of 49 new cases from Seattle. Blood, 48: 817, 1976.PubMedGoogle Scholar
  27. 27.
    Terasaki, P.I., Mottironi, V.D., and Barnett, E.V., Cytotoxins in disease. Autocytotoxins in lupus. N. Eng. J. Med., 283: 124, 1970.CrossRefGoogle Scholar
  28. 28.
    Thorsby, E., and Helgesen, A., Possible detection of sensitization against non-HLA histocompatibility antigens in Vitro. Symp. S er. Immunobiol. Stand., 78: 141, 1973.Google Scholar
  29. 29.
    Warren, R.P., Storb, R., Nguyen, P.D., and Thomas, E.D., The failure to demonstrate an involvement of human leukocyte group 5 antigens in graft—versus—host disease and marrow graft rejection. Transplantation, 24: 89, 1977.PubMedCrossRefGoogle Scholar
  30. 30.
    Warren, R.P., Storb, R., Weiden, P.L., Mickelson, E.M., and Thomas, E.D., Direct and antibody-dependent cell-mediated cytotoxicity against HLA identical sibling lymphocytes. Transplantation, 22: 631, 1976.PubMedCrossRefGoogle Scholar
  31. 31.
    Weiden, P.L., Storb, R., Schlichter, S., Warren, R.P., and Sale, G.E., Effects of six weekly transfusions on canine marrow grafts: Tests for sensitization and abrogation of sensitization by procarbazine and antithymocyte serum. J. Immunol., 777: 143, 1976.Google Scholar
  32. 32.
    Wilson, D.B., and Nowell, P.C., Quantitative studies on the mixed lymphocyte interaction in rats. V. Tempo and specificity of the proliferative response and the number of cells from immunized donors. J. Exp. Med., 755: 442, 1971.CrossRefGoogle Scholar

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© Springer-Verlag New York Inc. 1978

Authors and Affiliations

  • M. Jeannet
  • B. Speck

There are no affiliations available

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