Toluidine Blue as a Rapid Stain for Nerve Cell Bodies in Intact Ganglia

Abstract

The distribution of nerve cell bodies can be demonstrated in whole insect ganglia by staining with toluidine blue, making lengthy sectioning and reconstruction procedures (e.g., Cohen and Jacklett, 1967) unnecessary. This rapid and reliable method was developed as an adjunct to cobalt chloride staining of neurons (see Chaps. 17 and 19–21) for examining the relationship of cobalt-filled neurons to other nerve cells (Altman and Bell, 1973). It is also useful for locating positions of somata for intracellular recording, for comparative studies of neuron numbers and distribution, and for developmental work. It is excellent for preparing demonstration material for teaching and for practical classes in neurobiology.