Simple Axonal Filling of Neurons with Procion Yellow
The first successful use of the textile dye Procion yellow to fill individual neurons was reported by Kravitz et al. in 1968. Stretton and Kravitz (1968) then gave a fuller description of the method and of its use to reveal neuronal geometry in abdominal ganglia of the lobster. They had screened a total of 63 Procion dyes and related compounds and of these the four best for neurobiological purposes proved to be Procion brown H3RS, brilliant red H3BNS, navy blue H3RS, and yellow M4RS. Procion yellow was appreciably more fluorescent than the other three and was finally used exclusively. A full account of its selection and the development of the technique has been given by Stretton and Kravitz in the book on intracellular staining by Kater and Nicholson (1973). The dye could be introduced intracellularly with a micropipette and would spread throughout the neuron, revealing it under ultraviolet illumination virtually in its entirety. Iontophoresis was the method originally employed to inject the dye and rapidly became the standard procedure, though pressure injection has also sometimes been used (Remler et al., 1968; Milburn and Bentley, 1971).
KeywordsPetroleum Jelly Abdominal Ganglion Immersion Method Ultraviolet Illumination Staining Time
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