Biochemistry of Collagen with Special Reference to the Arterial Wall

  • R. Kent Rhodes


Investigations of the last decade have produced major advances in collagen biochemistry (for reviews see Bornstein and Sage 1980; Bornstein and Traub 1979; Eyre 1980; Prockop et al. 1979; Miller and Gay, in press). Among the most significant has been the discovery of the genetically distinct collagens that spawned numerous studies concerning the characterization and distribution of the various collagen types. Such studies have also begun to elucidate .some of the roles collagen can play in both normal and pathologic conditions (Gay and Miller 1978).


Collagen Type Bovine Aortic Endothelial Cell Type Versus Collagen Fibrous Plaque Amniotic Fluid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barnes MJ, Morton LF, Levene CI (1976) Synthesis of collagen types I and III by pig medial smooth muscle cells in culture. Biochem Biophys Res Commun 70: 339PubMedCrossRefGoogle Scholar
  2. Benditt EP (1977) The origin of atherosclerosis. Sci Am 236: 74Google Scholar
  3. Bentz H, Bachinger HP, Glanville R, Kuhn K (1978) Physical evidence for the assembly of A and B chains of human placental collagen in a single triple helix. Europ Biochem 92: 563CrossRefGoogle Scholar
  4. Bornstein P, Sage H (1980) Structurally distinct collagen types.An Rev Biochem 49: 957CrossRefGoogle Scholar
  5. Bornstein P, Traub W (1979) The chemistry and biology of collagen.In: Neurath H, Hill RL (eds) The Proteins, Vol IV.Academic Press, New YorkGoogle Scholar
  6. Brown RA, Shuttleworth CA, Weiss JB (1978) Three new a-chains of collagen from a non-basement membrane source. Biochem Biophys Res Commun 80: 866PubMedCrossRefGoogle Scholar
  7. Burgeson RE, El Adli FA, KaitilaII, Hollister OW (1976) Fetal membrane collagens: Identification of two new collagen alpha chains. Proc Natl Aca Sci USA 73: 2579CrossRefGoogle Scholar
  8. Burke JM, Bailan G. Ross R. Bornstein P (1977) Synthesis of types I and III procollagen by monkey aortic smooth muscle cells in vitro. Biochemistry 16: 3243PubMedCrossRefGoogle Scholar
  9. Chung E, Miller EJ (1974) Collagen polymorphism: Characterization of molecules with the chain composition [a1(III)]3 in human tissues. Science 183: 1200Google Scholar
  10. Chung E, Keele EM. Miller EJ (1974) Isolation and characterization of the cyanogen bromide peptides from the al(III) chain of human collagen. Biochemistry 13: 3459PubMedCrossRefGoogle Scholar
  11. Chung E, Rhodes RK. Miller EJ (1976) Isolation of three collagenous components of probable basement membrane origin from several tissues.Biochem Biophys Res Commun 71: 1167PubMedCrossRefGoogle Scholar
  12. Crouch E, Bornstein P (1978) Collagen synthesized by human amniotic fluid cells in culture: Characterization of a procollagen with three identical pro al(1) chains. Biochemistry 17: 5499Google Scholar
  13. Crouch E, Sage H, Bornstein P (1980) Structural basis for apparent heterogeneity of collagens in human basement membranes: Type IV procoUagen contains two distinct chains. Proc Natl Aca Sci USA 77: 745CrossRefGoogle Scholar
  14. Dixit SN (1979) Isolation and characterization of two a-chain size collagenous polypeptide chains C and D from glomerular basement membrane. FEBS Letters 106: 379PubMedCrossRefGoogle Scholar
  15. Eyre DR (1980) Collagen: Molecular diversity in the body’s protein scaffold. Science 207: 1315PubMedGoogle Scholar
  16. Furuto DK, Miller EJ (1980) Isolation of a unique collagenous fraction from limited pepsin digests of human placental tissue. Biol Chern 255: 290Google Scholar
  17. Gay S, Miller EJ (1978) Collagen in the Physiology and Pathology of Connective Tissue. Gustav Fischer, Stuttgart-New YorkGoogle Scholar
  18. Gay S, Miller EJ (1979) Characterization of lens capsule collagen: evidence for the presence of two unique chains in molecules derived from major basement membrane structures. Archiv Biochem Biophys 198: 370CrossRefGoogle Scholar
  19. Gay S, Balleisen L, Remberger K, Fietzek PP, Adelmann BC, Kuhn K (1975) Immunohistochemical evidence for the presence of collagen type III in human arterial walls, arterial thrombi, and in leucocytes, incubated with collagen in vitro. Klin Worchensch 53: 899CrossRefGoogle Scholar
  20. Gay S, Kresina TF, Gay R, Miller EJ, Montes LF (1979) Immunohistochemical demonstration of basement membrane collagen in normal human skin and in psoriasis. Cutaneous Pathol 6: 91CrossRefGoogle Scholar
  21. Glanville RW, Rauter A, Fietzek PP (1979) Isolation and characterization of a native placental basement-membrane collagen and its component chains. Europ Biochem 95: 383CrossRefGoogle Scholar
  22. Haralson MA, Mitchell WM, Rhodes RK, Kresina TF, Gay R, Miller EJ (1980) Chinese hamster lung cells synthesize and confine to the cellular domain a collagen comprised soley of B chains. Proc Natl Aca Sci USA in pressGoogle Scholar
  23. Howard BV, Macarak EJ, Gunson D, Kefalides NA (1976) Characterization of the collagen synthesized by endothelial cells in culture. Proc Natl Aca Sci USA 73: 2361CrossRefGoogle Scholar
  24. Jaffe EA, Minick CR, Adelman B, Becker CG, Nachman R (1976) Synthesis of basement membrane collagen by cultured human endothelial cells. Exper Med 144: 209CrossRefGoogle Scholar
  25. Kresina TF, Miller EJ (1979) Isolation and characterization of basement membrane collagen from human placental tissue. Evidence for the presence of two genetically-distinct collagen chains. Biochemistry 18: 3089PubMedCrossRefGoogle Scholar
  26. Layman DL, Epstein EJ, Dodson RF, Titus JL (1977) Biothesis of type I and III collagens by cultured smooth muscle cells from human aorta. Proc Natl Aca Sci USA 74: 671CrossRefGoogle Scholar
  27. Martin GM, Sprague CA (1973) Symposium on in vitro studies related to atherogenesis: Life histories of hyperplastoid cell lines from aorta and skin. Exper Mole Pathol 18: 125CrossRefGoogle Scholar
  28. Mayne R, Vail MS, Miller EJ (1975) Analysis of changes in collagen biosynthesis that occur when chick chondrocytes are grown in 5-bromo-2'-deoxyuridine. Proc Natl Aca Sci USA 72: 4511CrossRefGoogle Scholar
  29. Mayne R, Vail MS, Miller EJ, Blose SH, Chacko S (1977) Collagen polymorphisms in cell cultures derived from guinea pig aortic smooth muscle: Comparison with three populations of fibroblasts. Archiv Biochem Biophys 181: 462CrossRefGoogle Scholar
  30. Mayne R, Vail MS, Miller EJ (1978) Characterization of the collagen chains synthesized by cultured smooth muscle cells derived from rhesus monkey thoracic aorta. Biochemistry 17: 446PubMedCrossRefGoogle Scholar
  31. McCullagh KA, Balian G (1975) Collagen characterization and cell transformation in human atherosclerosis. Nature 258: 73PubMedCrossRefGoogle Scholar
  32. Miller EJ(1971) Isolation and characterization of a collagen from chick cartilage containing three identical a chains. Biochemistry 10: 1652PubMedCrossRefGoogle Scholar
  33. Miller EJ (1976) Biochemical characteristics and biological significance of the genetically-distinct collagens. Molec Cell Biochem 13: 165PubMedCrossRefGoogle Scholar
  34. Miller EJ, Gay S (in press) The multiple types and forms of collagen-an overview. In: Cunningham LW, Fredericksen D W (eds) Methods in Enzymology. Academic Press, New YorkGoogle Scholar
  35. Munksgaard EC, Rhodes M, Mayne R, Butler WT (1978) Collagen synthesis and secretion by rat incisor odontoblasts in organ culture. Europ Biochem 82: 609CrossRefGoogle Scholar
  36. Narayanan AS, Page RC, Kuzan F (1978) Collagens synthesized in vitro by diploid fibroblasts obtained from chronically inflamed human connective tissue. Lab Inves 39: 61Google Scholar
  37. Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA (1979) The biosynthesis of collagen and its disorders. N Eng J Med 301: 77CrossRefGoogle Scholar
  38. Rauterberg J, Allam S, Brehmer U, Wirth W, Hauss WH (1977) Characterization of the collagen synthesized by cultured human smooth muscle cells from fetal and adult aorta. HoppeSeyler&3x2019;s Zeitshr Physio Chern 358: 401CrossRefGoogle Scholar
  39. Rhodes RK, Miller EJ (1978) Physico-chemical characterization and molecular organization of the collagen A and B chains. Biochemistry 17: 3442PubMedCrossRefGoogle Scholar
  40. Ross R, Glomset JA (1976) The pathogenesis of atherosclerosis. N Eng J Med 295: 420CrossRefGoogle Scholar
  41. Sage H, Bornstein P (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen. Biochemistry 18: 3815PubMedCrossRefGoogle Scholar
  42. Sage H, Crouch E, Bornstein P (1979a) Collagen synthesis by bovine aortic endothelial cells in culture. Biochemistry 18: 5433PubMedCrossRefGoogle Scholar
  43. Sage H, Wordbury RG, Bornstein P (1979b) Structural studies on human type IV collagen. J Biol Chern 254: 9893Google Scholar
  44. Scott DM, Harwood R, Grant ME, Jackson DS (1977) Characterization of the major collagen species present in procine aortae and the synthesis of their precursors by smooth muscle cells in culture. Connective Tissue Res 5: 7CrossRefGoogle Scholar
  45. Smith GN Jr, Linsenmayer TF, Newsome DA (1976) Synthesis of type II collagen in vitro by embryonic chick neural retina tissue. Proc Nati Aca Sci USA 73: 4420CrossRefGoogle Scholar
  46. Timpl R, Martin GR, Bruckner P, Wick G, Wiedemann H (1978) Nature of the collagenous protein in a tumor basement membrane. Europ J Biochem 84: 43PubMedCrossRefGoogle Scholar
  47. Trelstad RL, Kang AH, Igarashi S, Gross J (1970) Isolation of two distinct collagens from chick cartilage. Biochemistry 9: 4993PubMedCrossRefGoogle Scholar
  48. Uitto J (1979) Collagen polymorphism: isolation and partial characterization of a1(I)-trimer molecules in normal human skin. Archiv Biochem Biophys 192: 371CrossRefGoogle Scholar
  49. Wohllebe M, Carmichael DJ (1978) Type-I-trimer and type-I collagen in neutral-salt-soluble lathyritic-rat dentine. Europ JBiochem 92: 183CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1981

Authors and Affiliations

  • R. Kent Rhodes

There are no affiliations available

Personalised recommendations