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Current Developments in Laser Microirradiation

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The Biomedical Laser

Abstract

Laser microirradiation was introduced in experimental cytology in 1962 by Bessis and his colleagues in Paris (Bessis et al, 1962). Since that time, numerous studies have been conducted using various laser sources to produce alterations at the subcellular level. These studies have been extensively reviewed over the past 10 years (see reviews by Berns and Salet, 1972; Moreno et al, 1969; Berns, 1974a, 1974b, 1975, 1978; Berns et al, 1976). The present review will briefly describe the studies discussed in the previous reviews and will concentrate on work conducted in our laboratory since that time. Because of space constraints, we will not review the important UV laser microbeam studies conducted on tissue culture nuclei by Cremer et al. (1976) and the laser microbeam studies conducted by Salet (1972) and Salet et al. (1979) on myocardial cell mitochondria.

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References

  • Adkisson KP, Baic D, Burgott S, Cheng WK, Berns MW (1973) Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. IV. Ultrastructural and cytochemical analysis of minimal lesions. J Mol Cell Cardiol 5:559–564.

    Article  Google Scholar 

  • Berns MW (1974a) Recent progress in laser microbeams. Int Rev Cytol 39:383–411.

    Article  Google Scholar 

  • Berns MW (1974b) Microbeams. In: Wolbarsht ML (ed) Laser Applications in Medicine and Biology, vol. 2. Plenum Press, New York, pp. 1–40.

    Google Scholar 

  • Berns MW (1974c) Directed chromosome loss by laser microirradiation. Science 186:700–705.

    Article  Google Scholar 

  • Berns MW (1975) Dissecting the cell with a laser microbeam. In: Joussot-Dubien J (ed) Lasers in Physical Chemistry and Biophysics. Elsevier Publishing Company, Amsterdam, pp. 389–401.

    Google Scholar 

  • Berns MW (1978) The laser microbeam as a probe for chromatin structure and function. In: Stein G, Stein J, and Kleinsmith L (eds) Methods in Cell Biology, vol. 18. Academic Press, New York, pp. 277–294.

    Google Scholar 

  • Berns MW, Cheng WK (1971) Are chromosome secondary constructions nucleolar organizers: A re-evaluation using a laser microbeam. Exp Cell Res 69:185–192.

    Article  Google Scholar 

  • Berns MW, Cheng WK (1973) Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. Pacemaker versus non-pacemaker cells. Life Sci 12:469–474.

    Article  Google Scholar 

  • Berns MW, Floyd AD (1971) Chromosome microdissection by laser: A functional cytochemical analysis. Exp Cell Res 67:305–310.

    Article  Google Scholar 

  • Berns MW, Richardson SM (1977) Continuation of mitosis after selective laser microbeam destruction of the centriolar region. J Cell Biol 75:977–982.

    Article  Google Scholar 

  • Berns MW, Salet C (1972) Laser microbeams for partial cell irradiation. Int Rev Cytol 33:131–154.

    Article  Google Scholar 

  • Berns MW, Olson RS, Rounds DE (1969)In vitro production of chromosomal lesions. Nature (Lond) 221:74–75.

    Article  Google Scholar 

  • Berns MW, Ohnuki Y, Rounds DE, Olson RS (1970a) Modification of nucleolar expression following laser microirradiation of chromosomes. Exp Cell Res 60:133–138.

    Article  Google Scholar 

  • Berns MW, Gamaleja N, Duffy C, Olson R, Rounds DE (1970b) Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. J Cell Physiol 76:207–214.

    Article  Google Scholar 

  • Berns MW, Cheng WK, Floyd AD, Ohnuki Y (1971) Chromosome lesions produced with an argon laser microbeam without dye sensitization. Science 171:903–905.

    Article  Google Scholar 

  • Berns MW, Gross DCL, Cheng WK, Woodring D (1972a) Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. II. Correlation of morphology and function in single irradiated cells. J Mol Cell Cardiol 4:71–83.

    Article  Google Scholar 

  • Berns MW, Gross DCL, Cheng WK (1972b) Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. III. Irradiation of multicellular networks. J Mol Cell Cardiol 4:427–434.

    Article  Google Scholar 

  • Berns MW, Rattner JB, Meredith S, Witter M (1976) Current laser microirradiation studies. Ann NY Acad Sci 267:160–175.

    Article  Google Scholar 

  • Berns MW, Rattner JB, Brenner S, Meredith S (1977) The role of the centriolar region in animal cell mitosis: A laser microbeam study. J Cell Biol 72:351–367.

    Article  Google Scholar 

  • Berns MW, Chong LK, Hammer-Wilson M, Miller K, Siemens A (1979) Genetic microsurgery by laser: Establishment of a clonal population of rat kangaroo cells (PTK2) with a directed deficiency in a chromosomal nucleolar organizer. Chromosoma 73:1–8.

    Article  Google Scholar 

  • Bessis M, Gires F, Mayer G, Nomarski G (1962) Irradiation des organites cellulaires á l’aide d’un laser á rubis. C R Acad Sci [D] 225:1010–1012.

    Google Scholar 

  • Branch AB, Berns MW (1976) Nucleoli and ploidy in Potorous cells (PTK2)in vitro. Chromosoma 56:33–40.

    Article  Google Scholar 

  • Brenner S, Branch A, Meredith S, Berns MW (1977) The absence of centrioles from spindle poles of PTK2 undergoing meiotic-like reduction division in vitro. J Cell Biol 72:368–379.

    Article  Google Scholar 

  • Brenner SL, Liaw L-H, Berns MW (in press) Laser microirradiation of kinetochores in mitotic PtK2 cells: Chromatid separation and micronucleus formation. Cell Biophysics.

    Google Scholar 

  • Brinkley L, Berns MW (1974) Laser microdissection of actinomyein D segregated nucleoli. Exp Cell Res 87:417–422.

    Article  Google Scholar 

  • Brinkley BR, Murphy P, Richardson LC (1967) Procedure for embedding in situ selected cells cultured in vitro. J Cell Biol 35:279–283.

    Article  Google Scholar 

  • Burt JM, Strahs KR, Berns MW (1979) Correlation of cell surface alterations with contractile response in laser microbeam irradiated myocardial cells: A scanning electron microscope study. Exp Cell Res 118:341–351.

    Article  Google Scholar 

  • Cremer C, Cremer T, Zorn C, Schoeller L (1976) Effects of laser UV-microirradiation (A = 2573A) on proliferation of Chinese hamster cells. Radiat Res 66:106–121.

    Article  Google Scholar 

  • Gould RR, Borisy GG (1977) The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation. J Cell Biol 73:601–615.

    Article  Google Scholar 

  • Kitzes M, Twiggs G, Berns MW (1977) Alteration of membrane electrical activity in ray myocardial cells following selective laser microbeam irradiation. J Cell Physiol 93:99–104.

    Article  Google Scholar 

  • Mark GE, Strasser FF (1966) Pacemaker activity and mitosis in cultures of newborn rat heart ventricle cells. Exp Cell Res 44:217–233.

    Article  Google Scholar 

  • McNeill PA, Berns MW (1979a) Laser microirradiation of a single kinetochore in mitotic PTK2 cells. In vitro 15:206.

    Google Scholar 

  • McNeill PA, Berns MW (1979b) The role of the kinetochore in the initiation of anaphase movement, studied by laser microirradiation. J Cell Biol 83:374a.

    Google Scholar 

  • McNeill PA, Berns MW (in press) Chromosome behavior following laser microirradiation of a single kinetochore in mitotic PtK2 cells. J Cell Biol.

    Google Scholar 

  • Meredith S, Berns MW (1976) Light and electron microscopy of laser microirradiated nucleoli and nucleoplasm in tissue culure cells. J Morphol 150:785–804.

    Article  Google Scholar 

  • Moreno G (1971) Effects of UV microirradiation on different parts of the cell. II. Cytological observation and unscheduled DNA synthesis after partial nuclear irradiation. Exp Cell Res 65:129–139.

    Article  Google Scholar 

  • Moreno G, Lutz M, Bessis M (1969) Partial cell irradiation by ultraviolet and visible light: Convention and laser sources. Int Rev Exp Pathol 7:99–137.

    Google Scholar 

  • Nicklas RB (1961) Recurrent pole to pole movements of the sex chromosome during prometaphase I in Melanoplus differentialis spermatocytes. Chromosoma 12:97–115.

    Article  Google Scholar 

  • Ohnuki Y, Olson RS, Rounds DE, Berns MW (1972) Laser microbeam irradiation of the juxtanucleolar region of prophase nucleolar chromosomes. Exp Cell Res 71:132–144.

    Article  Google Scholar 

  • Perry RP, Hell A, Errera M (1961) The role of the nucleolus in ribonucleic acid and protein synthesis. I. Incorporation of cytidine into normal and nucleolar inactivated HeLa cells. Biochim Biophys Acta 49:47–57.

    Article  Google Scholar 

  • Peterson SP, Berns MW (1978a) Chromatin influence on the function and formation of the nuclear envelope shown by laser-induced psoralen photoreaction. J Cell Sci 32:197–213.

    Google Scholar 

  • Peterson SP, Berns MW (1978b) Evidence for centriolar region RNA functioning in spindle formation in dividing PTK2 cells. J Cell Sci 34:289–301.

    Google Scholar 

  • Rattner JB, Berns MW (1974) Light and electron microscopy of laser microirradiated chromosomes. J Cell Biol 62:526–533.

    Article  Google Scholar 

  • Rattner JB, Berns MW (1976a) Centriole behavior in early mitosis of rat kangaroo cells (PTK2). Chromosoma 54:387–395.

    Article  Google Scholar 

  • Rattner JB, Berns MW (1976b) Distribution of microtubules during centriole separation in rat kangaroo (Potorous) cells. Cytobios 15:37–43.

    Google Scholar 

  • Rattner JB, Lifsics M, Meredith S, Berns MW (1976) Argon laser microirradiation of mitochondria in rat myocardial cells. VI. Correlation of contractility and ultrastructure. J Mol Cell Cardiol 8:239–248.

    Article  Google Scholar 

  • Sakharov VN, Voronkova LN (1966) Consequences of UV-microbeam irradiation of the nucleoli of living cell. Genetika 6:144–148.

    Google Scholar 

  • Salet C (1972) A study of beating frequency of a single myocardial cell. I. Q-switched laser microirradiation of mitochondria. Exp Cell Res 73:360–366.

    Article  Google Scholar 

  • Salet C, Moreno G, Vinzens FA (1979) A study of beating frequency of a single myocardial cell. III. Laser microirradiation of mitochondria in the presence of KCN or ATP. Exp Cell Res 120:25–29.

    Article  Google Scholar 

  • Strahs KR, Berns MW (1979) Laser microirradiation of stress fibers and intermediate filaments in non-muscle cells from cultured rat heart. Exp Cell Res 119:31–45.

    Article  Google Scholar 

  • Strahs KR, Burt JM, Berns MW (1978) Contractility changes in cultured cardiac cells following laser microirradiation of myofibrils and the cell surface. Exp Cell Res 113:75–83.

    Article  Google Scholar 

  • Waymire K, Kitzes M, Meredith S, Twiggs G, Berns MW (1976) Argon laser microirradiation of mitochondria in rat myocardial cells in tissue culture. VII. Fibrillation in ventricle and auricle cells. J Cell Physiol 89:345–354.

    Article  Google Scholar 

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© 1981 Springer-Verlag New York Inc.

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Berns, M.W. et al. (1981). Current Developments in Laser Microirradiation. In: Goldman, L. (eds) The Biomedical Laser. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5922-0_6

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  • DOI: https://doi.org/10.1007/978-1-4612-5922-0_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-5924-4

  • Online ISBN: 978-1-4612-5922-0

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