Sucrose Gradients: An Assay for DNA Damage
Exposure of cells to various physical and chemical agents often leads to damage in the cellular genetic material, DNA. Ionizing radiation, UV light, fluorescent light, microwaves, ultrasound, and heat have all been shown to induce DNA breaks. Many chemicals, including pharmacological agents, have also been found to cause a variety of injuries in DNA. Unrepaired or improperly repaired DNA damage may lead to a mutation, and, since mutagenesis and carcinogenesis are believed to be closely related, it can be assumed that agents which damage DNA are also capable of inducing human cancer.
KeywordsSucrose Gradient Number Average Molecular Weight Preparative Ultracentrifuge Alkali Condition CH282 Cell
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