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DNA Repair Synthesis (UDS) as an in vitro and in vivo Bioassay to Detect Precarcinogens, Ultimate Carcinogens, and Organotropic Carcinogens

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Short-Term Tests for Chemical Carcinogens

Part of the book series: Topics in Environmental Physiology and Medicine ((TEPHY))

Abstract

Since Rasmussen and Painter (1966) first observed an unscheduled incorporation of tritiated thymidine (3HTdR) by UV-irradiated cells, this method for assessing DNA repair synthesis has been used for several purposes. These include detection of mutagenic and carcinogenic activities of chemicals or complex mixtures, detection of DNA repair inhibitors, estimation of organotropic actions of carcinogens, and identification of individuals with DNA repair deficiencies. In this paper we will examine the usefulness of DNA repair synthesis as an economical and rapidly performed bioassay method for detecting DNA-damaging agents and, by implication, carcinogens and mutagens. We will deal with the potential usefulness of this technique as a tool to uncover the action of organotropic carcinogens in various in vivo and in vitro systems. The mechanism of DNA repair has been comprehensively reviewed by others (Hanawalt et al., 1978, 1979; Trosko and Chu, 1975; Trosko et al., 1977), and an extensive examination of this aspect is beyond the scope of this paper.

Supported by research grants from the National Cancer Institute of Canada, Natural Sciences and Engineering Research Council of Canada, and the British Columbia Health Care Research Foundation. The authors wish to thank Miss Rosemary Johnson for typing this manuscript.

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Authors
  1. Hans F. Stich
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  2. Richard H. C. San
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  3. Hugh J. Freeman
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Editors and Affiliations

  1. Environmental Carcinogenesis Unit, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

    H. F. Stich (Head) (Head)

  2. Carcinogen Testing Laboratory, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

    R. H. C. San (Chief) (Chief)

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Stich, H.F., San, R.H.C., Freeman, H.J. (1981). DNA Repair Synthesis (UDS) as an in vitro and in vivo Bioassay to Detect Precarcinogens, Ultimate Carcinogens, and Organotropic Carcinogens. In: Stich, H.F., San, R.H.C. (eds) Short-Term Tests for Chemical Carcinogens. Topics in Environmental Physiology and Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5847-6_7

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