An Escherichia coli Differential Killing Test for Carcinogens Based on a uvrA recA lexA Triple Mutant
Virtually all short-term tests for mutagens and carcinogens detect the ability of an agent to damage DNA, either directly or following metabolic activation. A differential killing assay demonstrates this by increased killing of a strain unable to repair DNA damage, as compared to an isogenic repair-proficient strain. The best known of such tests are the rec-assay of Kada et al. (1972) and the pol-assay of Slater et al. (1971). We believe that the test described here may have two advantages over other assays.
KeywordsToxicity Agar Dimethyl Resis Diethyl
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