An Escherichia coli Differential Killing Test for Carcinogens Based on a uvrA recA lexA Triple Mutant
Virtually all short-term tests for mutagens and carcinogens detect the ability of an agent to damage DNA, either directly or following metabolic activation. A differential killing assay demonstrates this by increased killing of a strain unable to repair DNA damage, as compared to an isogenic repair-proficient strain. The best known of such tests are the rec-assay of Kada et al. (1972) and the pol-assay of Slater et al. (1971). We believe that the test described here may have two advantages over other assays.
KeywordsTest Agent Ames Test Mutation Assay Killing Assay Small Test Tube
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