An Escherichia coli Differential Killing Test for Carcinogens Based on a uvrA recA lexA Triple Mutant

  • M. H. L. Green
  • D. J. Tweats
Conference paper
Part of the Topics in Environmental Physiology and Medicine book series (TEPHY)


Virtually all short-term tests for mutagens and carcinogens detect the ability of an agent to damage DNA, either directly or following metabolic activation. A differential killing assay demonstrates this by increased killing of a strain unable to repair DNA damage, as compared to an isogenic repair-proficient strain. The best known of such tests are the rec-assay of Kada et al. (1972) and the pol-assay of Slater et al. (1971). We believe that the test described here may have two advantages over other assays.


Test Agent Ames Test Mutation Assay Killing Assay Small Test Tube 
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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • M. H. L. Green
  • D. J. Tweats

There are no affiliations available

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