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Mutagenesis Studies in Diploid Human Cells with Different DNA-Repair Capacities

  • Conference paper
Short-Term Tests for Chemical Carcinogens

Part of the book series: Topics in Environmental Physiology and Medicine ((TEPHY))

Abstract

Human diploid fibroblasts in culture can be very useful for examining the cytotoxic and mutagenic effects of environmental agents (Albertini and DeMars, 1973; Buchwald, 1977; Cox and Masson 1976; Glover et al., 1979; Jacobs and DeMars, 1978; Maher and McCormick, 1976, 1980; Maher and Wessel, 1975; Maher et al., 1975, 1976a,b,c, 1977, 1979; Mankovitz et al., 1974). Studies with these human cells provide a bridge between studies conducted with animal cells in culture and whole animals, on the one hand, and epidemiological studies of the accidental exposure of humans to various agents on the other. In addition, the use of human cells makes it possible to carry out comparative studies designed to elucidate the role of DNA repair in somatic cell mutagenesis (and carcinogenesis) because of the availability of repair-deficient strains derived from individuals with various inherited DNA-repair-deficiency diseases (Fujiwara et al., 1977; German, 1972; Lehmann et al., 1975; Robbins et al., 1974; Taylor et al, 1975).

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Abbreviations

NF:

(normal fibroblasts) i.e., diploid fibroblasts derived from normal individuals

XP:

xeroderma pigmentosum

AG, 8:

azaguanine

TG, 6:

thioguanine

HX:

hypoxanthine

HPRT:

hypoxanthine(guanine) phosphoribosyltransferase

PBS:

phosphate buffered saline, pH 7.2.

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Authors
  1. J. Justin McCormick
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  2. Veronica M. Maher
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Editor information

Editors and Affiliations

  1. Environmental Carcinogenesis Unit, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

    H. F. Stich (Head) (Head)

  2. Carcinogen Testing Laboratory, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

    R. H. C. San (Chief) (Chief)

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© 1981 Springer-Verlag New York Inc.

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McCormick, J.J., Maher, V.M. (1981). Mutagenesis Studies in Diploid Human Cells with Different DNA-Repair Capacities. In: Stich, H.F., San, R.H.C. (eds) Short-Term Tests for Chemical Carcinogens. Topics in Environmental Physiology and Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5847-6_24

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  • DOI: https://doi.org/10.1007/978-1-4612-5847-6_24

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-5849-0

  • Online ISBN: 978-1-4612-5847-6

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