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Applications of the Salmonella/Microsome Assay

  • Conference paper
Short-Term Tests for Chemical Carcinogens

Part of the book series: Topics in Environmental Physiology and Medicine ((TEPHY))

Abstract

The Salmonella/microsome assay has been described in detail (Ames et al., 1973, 1975b; McCann et al., 1975b). I will present some rationale for using this procedure as well as the results obtained in testing some chemicals.

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References

  • Ames, B.N. (1971): The detection of chemical mutagens with enteric bacteria. In: A. Hol- laender (ed.), Chemical Mutagens: Principles and Methods for Their Detection, Vol. 1. New York: Plenum Press, pp. 267–282.

    Google Scholar 

  • Ames, B.N., Durston, W.E., Yamasaki, E., Lee, F.D. (1973): Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. USA 72 2423–2427.

    Article  Google Scholar 

  • Ames, B.N., Kämmen, H.O., Yamasaki, E. (1975a): Hair dyes are mutagenic: Identification of a variety of mutagenic ingredients. Proc. Natl. Acad. Sci. USA 72: 2423–2427.

    Article  PubMed  CAS  Google Scholar 

  • Ames, B.N., McCann, J., Yamasaki, E. (1975b): Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutat. Res. 31: 347–364.

    PubMed  CAS  Google Scholar 

  • Baden, J.M., Brinkenhoff, M., Wharton, R.S., Hitt, B., Simmon, V.F., Mazze, R.I. (1976): Mutagenicity of volatile anesthetics: Halothane. Anesthesiology 45: 311–318.

    Article  PubMed  CAS  Google Scholar 

  • Baden, J.M., Kelley, M.J., Wharton, R.S., Hitt, B.A., Simmon, V.F., Mazze, R.I. (1977): Mutagenicity of halogenated ether anesthetics. Anesthesiology 46: 346–350.

    Article  PubMed  CAS  Google Scholar 

  • Baden, J., Kelley, M.J., Mazze, R.I., Simmon, V.F. (1979): Mutagenicity of inhalation anesthetics; nitrous oxide, cyclopropane, trich-loroethylene and divinyl ether. Br. J. Anaesth. 51: 417–421.

    Article  PubMed  CAS  Google Scholar 

  • Bartsch, H., Malaveille, C., Montesano, R., Tomatis, L. (1975): Tissue-mediated mutagenicity of vinylidene chloride and 2-chloro-butadiene in Salmonella typhimurium. Nature (Lond.) 255: 641–643.

    Article  CAS  Google Scholar 

  • Blum, A., Ames, B.N. (1977): Flame-retardent additives as possible cancer hazards. The main flame retardant in children’s pajamas is a mutagen and should not be used. Science 195: 17–23.

    Article  PubMed  CAS  Google Scholar 

  • Bridges, B.A. (1978): Detection of volatile liquid mutagens with bacteria: Experiments with dichlorvos and epichlorohydrin. Mutat. Res. 54: 367–371.

    PubMed  CAS  Google Scholar 

  • Dunkel, V.C., Simmon, V.F. (1980): Mutagenic activity of chemicals previously tested for carcinogenicity in the National Cancer Institute Bioassay Program. In: R. Montesano, H. Bartsch, L. Tomatis (eds.), Molecular and Cellular Aspects of Carcinogen Screening Tests. Lyon: IARC Scientific Publications No. 27.

    Google Scholar 

  • Hiatt, H.H., Watson, J.D., Winsten, J.A. (eds.) (1977): Origins of Human Cancer, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  • Huisingh, J., Bradow, R., Fungers, R., Claxton, L., Zweidinger, R., Tejada, S., Bumgarner, J., Duffield, F., Waters, M., Simmon, V.F., Hard, C., Rodriguez, C., Snow, L. (1979): Application of bioassay to the characterization of diesel particle emissions. In: M.D. Waters, et al. (eds.), Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Environmental Science Research Series, Vol. 15, pp. 381–418.

    Google Scholar 

  • Legator, M.S., Connor, T.H., Stoeckel, M. (1975): Detection of mutagenic activity of metronidazole and niridazole in body fluids of humans and mice. Science 188: 118–119.

    Article  Google Scholar 

  • Lehr, R.E., Schaefer-Ridder, M., Jerina, D.M. (1977): Synthesis and properties of the vicinal transdihydrodials of anthracene, phenanthrene and benzo(a)anthracene. J. Org. Chem. 42: 744–746.

    Article  Google Scholar 

  • Loper, J.C., Lang, D.R., Smith, C.C. (1978): Mutagenicity of complex mixtures from drinking water. In: R.L. Jolley, H. Gorchev, and D.H. Hamilton, Jr. (eds.), Water Chlorination: Environmental Impact and Health Effects, Vol. 2. Ann Arbor, Michigan, Ann Arbor: Science Publishers, pp. 433–450.

    Google Scholar 

  • Malaveille, C., Bartsch, H., Barbin, A., Camus, A.M., Montesano, R., Croisy, A., and Jacquignon, P. (1975): Mutagenicity of vinyl chloride, chloroethylene oxide, chlorace-taldehyde and chloroethanol. Biochem. Biophys. Res. Commun. 63: 363–370.

    Article  PubMed  CAS  Google Scholar 

  • McCann, J., Ames, B.N. (1976): Detection of carcinogens as mutagens in the Sal- monella/microsome test: Assay of 300 chemicals. Discussion. Proc. Natl. Acad. Sei. USA 73: 950–954.

    Article  CAS  Google Scholar 

  • McCann, J., Choi, E., Yamasaki, E., Ames, BN. (1975a): Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals. Proc. Natl. Acad. Sci. USA 72: 5135–5139.

    Article  PubMed  CAS  Google Scholar 

  • McCann, J., Spingarn, N.E., Kobori, J., Ames, D.N. (1975b): Detection of carcinogens as mutagens: Bacterial tester strains with R factor plasmids. Proc. Natl. Acad. Sci. USA 72: 979–983.

    Article  PubMed  CAS  Google Scholar 

  • Prival, M.J., McCoy, E., Gutter, B., Rosenkranz, H.S. (1977): Tris (2,3-dibromopropyl) phosphate: mutagenicity of a widely used flame retardant. Science 195: 76–78.

    Article  PubMed  CAS  Google Scholar 

  • Rannug, U., Johansson, A., Ramel, C., and Wachtmeister, C.A. (1974): Mutagenicity of vinyl chloride after metabolic activation. Ambio 3: 194–197.

    CAS  Google Scholar 

  • Simmon, V.F. (1977): Structural correlations of carcinogenic and mutagenic alkyl halides. In: I.M. Asher and C. Zervos (eds.), Structural Correlations of Carcinogenesis and Mutagenesis: A Guide to Testing Priorities. Proceedings of Second FDA Office of Science Summer Symposium, pp. 163–171, Office of Science, Food and Drug Administration.

    Google Scholar 

  • Simmon, V.F. (1979): In vitro mutagenicity assays of chemical carcinogens and related compounds with Salmonella typhimurium. J. Natl. Cancer Inst. 62:893–899.

    PubMed  CAS  Google Scholar 

  • Simmon, V.F., Kauhanen, K., Tardiff, R.G. (1977): Mutagenic activities of chemicals identified in drinking water. In: D. Scott, B.A. Bridges, and F.H. Sobels (eds.), Progress in Genetic Toxicology, Amsterdam: Elsevier/North-Holland Biomedical Press, pp. 249–258.

    Google Scholar 

  • Wood, A.W., Levin, W., Thomas, P.E., Ryan, D., Karle, J.M., Yagi, H., Jerina, M.D., Conney, A.R. (1978): Metabolic activation of dibenzo (a,h) anthracene and its dihydrodiols to bacterial mutagens. Cancer Res. 39: 1967–1973.

    Google Scholar 

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Authors
  1. Vincent F. Simmon
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Editor information

Editors and Affiliations

  1. Environmental Carcinogenesis Unit, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

    H. F. Stich (Head) (Head)

  2. Carcinogen Testing Laboratory, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada

    R. H. C. San (Chief) (Chief)

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© 1981 Springer-Verlag New York Inc.

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Simmon, V.F. (1981). Applications of the Salmonella/Microsome Assay. In: Stich, H.F., San, R.H.C. (eds) Short-Term Tests for Chemical Carcinogens. Topics in Environmental Physiology and Medicine. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5847-6_12

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  • DOI: https://doi.org/10.1007/978-1-4612-5847-6_12

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-5849-0

  • Online ISBN: 978-1-4612-5847-6

  • eBook Packages: Springer Book Archive

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