A Hydrophobic Tryptic Peptide from Bovine Brain
The major protein of central nervous system myelin is a hydrophobic intrinsic membrane protein designated proteolipid protein. The proteolipid apoprotein, devoid of complex lipids is completely soluble in chloroform-methanol mixtures but can be converted to a water soluble form. The primary structure of this protein has been difficult to obtain because of problems related to solubility and aggregation. In the present study we have used a combination of solid phase Edman degradation and mass spectrometry to obtain the primary structure of a major, chloroform-soluble tryptic peptide which is located in the COOH-terminal region of the protein.