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A Hydrophobic Tryptic Peptide from Bovine Brain

  • M. B. Lees
  • B. Chao
  • R. Laursen
  • J. L’Italian
  • J. Evans
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)

Abstract

The major protein of central nervous system myelin is a hydrophobic intrinsic membrane protein designated proteolipid protein. The proteolipid apoprotein, devoid of complex lipids is completely soluble in chloroform-methanol mixtures but can be converted to a water soluble form. The primary structure of this protein has been difficult to obtain because of problems related to solubility and aggregation. In the present study we have used a combination of solid phase Edman degradation and mass spectrometry to obtain the primary structure of a major, chloroform-soluble tryptic peptide which is located in the COOH-terminal region of the protein.

Keywords

Primary Structure Tryptic Peptide Total Amino Acid Bovine Brain Complex Lipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • M. B. Lees
    • 1
  • B. Chao
    • 1
  • R. Laursen
    • 2
  • J. L’Italian
    • 2
  • J. Evans
    • 1
  1. 1.E. K. Shriver CenterWalthamUSA
  2. 2.Boston UniversityBostonUSA

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