A Hydrophobic Tryptic Peptide from Bovine Brain

  • M. B. Lees
  • B. Chao
  • R. Laursen
  • J. L’Italian
  • J. Evans
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)

Abstract

The major protein of central nervous system myelin is a hydrophobic intrinsic membrane protein designated proteolipid protein. The proteolipid apoprotein, devoid of complex lipids is completely soluble in chloroform-methanol mixtures but can be converted to a water soluble form. The primary structure of this protein has been difficult to obtain because of problems related to solubility and aggregation. In the present study we have used a combination of solid phase Edman degradation and mass spectrometry to obtain the primary structure of a major, chloroform-soluble tryptic peptide which is located in the COOH-terminal region of the protein.

Keywords

HPLC Chloroform Trypsin Chymotrypsin Eluant 

Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • M. B. Lees
    • 1
  • B. Chao
    • 1
  • R. Laursen
    • 2
  • J. L’Italian
    • 2
  • J. Evans
    • 1
  1. 1.E. K. Shriver CenterWalthamUSA
  2. 2.Boston UniversityBostonUSA

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