Abstract
It has now been nearly 30 years since Sanger and his colleagues first showed that a protein (insulin) has a unique amino acid sequence1. The tools they developed to generate fragments, to isolate small peptides, to determine amino acid compositions, and to establish end groups defined the frontier of protein chemistry at that time, but have been replaced over the years by procedures with greater efficiency, speed and sensitivity. Moore, Stein and their colleagues2,3 introduced more quantitative procedures. Edman replaced end group analysis with the stepwise degradation procedure bearing his name4 and led us towards automated instruments which allow us to perceive answers before we forget the questions. It is becoming routine that the sequence of a purified protein is determined as a normal part of a complete program to study the structural basis of its function and control.
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References
Sanger, F., and Tuppy, H. (1951) Biochem. J., 49, 463.
Spackman, D.H., Stein, W.H., and Morre, S. (1958) Anal. Chem., 30, 1190.
Hirs, C.H.W., Moore, S., and Stein, W.H. (1960) J. Biol. Chem., 235, 633.
Edman, P. (1956) Acta Chem. Scand. 10, 761.
Edman, P. (1977) Carlsberg Res. Commun. 42, 1.
Walsh, K.A., Ericsson, L.H., Parmelee, D.C., and Titani, K. (1981) Ann. Rev. Biochem. 50, 261.
Walsh, K.A., Ericsson, L.H., and Titani, K. (1978). in Versatility of Proteins, Li, C.H., ed., Academic Press p. 39.
Fowler, A.V., and Zabin, I. (1978) J. Biol. Chem. 253, 5521.
Ovchinnikov, Y.A., Monastyrskaya, G.S., Gubanov, V.V., Guryev, S.O., Chertov, O.Y., Modyanov, N.N., Grinkevich, V.A., Makarova, I.A., Marchenko, T.V., Polovnikova, I. N., Lipkin, V.M. and Sverdlov, E.D. (1981) Eur. J. Biochem. 116, 621.
Fietzek, P.P., Allmann, H., Rauterberg, J., Henkel, W., Wachter, E., and Kuhn, K. (1979) Hoppe-Seyler’s Z. Physiol. Chem. 360, 809.
Uy, R., and Wold, F. (1977) Science 198, 890.
Morris, H.R. (1979) Phil. Trans. R, Soc. Lond. A. 293, 39.
Morris, H.R., Panico, M., Barber, M., Bordoli, R.S., Sedgwick, R.D. and Tyler, A. (1981) Biochem. Biophys. Res. Commun. 101, 623.
Williams, D.H., Bojesen, G., Auffret, A.D., and Taylor, C.E. (1981) FEBS Lett. 128, 37.
Nebelin, E. (1980) in Methods in Peptide and Protein Sequence Analysis, Birr, C., ed., Elsevier/North Holland Press p. 173.
Bornstein, P., and Balian, G. (1977) Methods Enzymol. 47, 132.
Landon, M. (1977) Methods Enzymol. 47, 145.
Bloxham, D.P., Parmelee, D.C., Kumar, S., Wade, R.D. Ericsson, L.H., Neurath, H., Walsh, K.A., and Titani, K. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, in press.
Tarr, G.E., Beecher, J.F., Bell, M., and McKean, D.J. (1978) Anal. Biochem. 84, 622.
Klapper, D.G., Wilde, C.E. III, and Capra, J.D. (1978) Anal. Biochem. 85, 126.
Hewick, R.M., Hunkapiller, M.W., Hood, L.E., and Dreyer, W.J. (1981) J. Biol. Chem. 256, 7990.
Laursen, R.A., (1971) Eur. J. Biochem. 20, 89.
Wittman-Liebold, B., (1980) in Polypeptide Hormones, Beers, R. F. Jr., Bassett, G., eds., Raven Press, p. 87.
Hunkapiller, M.W., and Hood, L.E. (1978) Biochemistry 17, 2124.
Hunkapiller, M.W., and Hood, L.E. (1980) Science 207, 523.
Koide, A., Titani, K., Ericsson, L.H., Kumar, S., Neurath, H., and Walsh, K.A. (1978) Biochemistry 17, 5657.
Boosman, A. (1980) in Methods in Peptide and Protein Sequence Analysis, Birr, C., Ed., Elsevier/North Holland Press, p. 513.
Brandt, W.F., Henschen, A., and von Holt, C. (1980) Hoppe-Seyler’s Z. Physiol. Chem. 361, 943.
Frank, G. (1979) Hoppe-Seyler’s Z. Physiol. Chem. 360, 997.
Brown, A.S., Bennett, J.C., Morgan, P.H. and Mole, J. E. (1981) Anal. Biochem. 112, 158.
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Walsh, K.A. (1982). Strategic Approaches to Sequence Analysis. In: Elzinga, M. (eds) Methods in Protein Sequence Analysis. Experimental Biology and Medicine, vol 3. Humana Press. https://doi.org/10.1007/978-1-4612-5832-2_3
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DOI: https://doi.org/10.1007/978-1-4612-5832-2_3
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