A New Computer-Aided Method for Sequencing a Polypeptide from the Masses and Edman-Degradation of its Constituent Peptide Fragments

  • Yasutsugu Shimonishi
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)


Recently Edman degradation [1] has been greatly improved and refined for determination of the amino acid sequences of peptides and proteins in subnanomolar quantities, particularly by use of an improved automated sequencer [2]. However, the method generally requires the purification of peptide mixtures generated from the polypeptide or protein to be sequenced. During the past 3 years, we elaborated a method [3,4] for sequencing peptide mixtures, that does not require their purification, by a combination of field- desorption mass spectrometry (FD-MS) and Edman degradation, because the separation and purification of peptide mixtures are still time consuming and tedious procedures.


Tryptic Peptide Peptide Fragment Peptide Mixture Edman Degradation Desorption Mass Spectrometry 
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Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • Yasutsugu Shimonishi
    • 1
  1. 1.Institute for Protein ResearchOsaka UniversitySuita, OsakaJapan

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