Advertisement

A New Computer-Aided Method for Sequencing a Polypeptide from the Masses and Edman-Degradation of its Constituent Peptide Fragments

  • Yasutsugu Shimonishi
Part of the Experimental Biology and Medicine book series (EBAM, volume 3)

Abstract

Recently Edman degradation [1] has been greatly improved and refined for determination of the amino acid sequences of peptides and proteins in subnanomolar quantities, particularly by use of an improved automated sequencer [2]. However, the method generally requires the purification of peptide mixtures generated from the polypeptide or protein to be sequenced. During the past 3 years, we elaborated a method [3,4] for sequencing peptide mixtures, that does not require their purification, by a combination of field- desorption mass spectrometry (FD-MS) and Edman degradation, because the separation and purification of peptide mixtures are still time consuming and tedious procedures.

Keywords

Tryptic Peptide Peptide Fragment Peptide Mixture Edman Degradation Desorption Mass Spectrometry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Edman, P. and Henschen, A. (1975) in Protein Sequence Determination, Needlemann,S. B. ed., 2nd edn., Springer-Verlag, Berlin, pp. 232–279.Google Scholar
  2. [2]
    Hunkapiller, M.W. and Hood, L.E. (1980) Science 207, 523.PubMedCrossRefGoogle Scholar
  3. [3]
    Shimonishi, Y., Hong, Y.-M., Matsuo, T., Katakuse, I. and Matsuda, H.(1979) Chem.Lett. 1369.Google Scholar
  4. [4]
    Shimonishi, Y., Hong, Y.-M., Kitagishi, T., Matsuo, T., Matsuda, H. and Katakuse, I. (1980) Eur. J. Biochem. 112, 251.PubMedCrossRefGoogle Scholar
  5. [5]
    Kitagishi, T., Hong, Y.-M. and Shimonishi, Y. (1980) Int. J. Pept. Protein Res. 17, 436.CrossRefGoogle Scholar
  6. [6]
    Kitagishi, T., Hong, Y.-M., Takao, T., Aimoto, S. and Shimonishi, Y. Bull. Chem. Soc. Jpn. to be submitted.Google Scholar
  7. [7]
    Matsuda, H. (1976) Atomic Masses Fundam. Constants 5, 185.Google Scholar
  8. [8]
    Zimmerman, C.L., Appella, E. and Pisano, J.J. (1977) Anal.Biochem. 77, 569.PubMedCrossRefGoogle Scholar
  9. [9]
    Morita, T., Hara, S. and Matsushima, Y. (1978) J. Biochem. (Tokyo) 83, 893.Google Scholar

Copyright information

© The HUMANA Press Inc. 1982

Authors and Affiliations

  • Yasutsugu Shimonishi
    • 1
  1. 1.Institute for Protein ResearchOsaka UniversitySuita, OsakaJapan

Personalised recommendations