Characterization and Isolation of Dopamine-Binding Proteins in Rat CNS by Gel Electrophoresis & Affinity Chromatography

  • Louise L. Hsu
  • Kayoko Moroi
  • Angelos E. Halaris
Part of the Experimental and Clinical Neuroscience book series (ECN)


During the last decade, neurotransmitter receptors in the central nervous system have received considerable attention from investigators in search of unknown factors that may be involved in the etiology of various neurological and psychiatric disorders. In particular, dopamine (DA) receptors have been shown to be related to tardive dyskinesia (Baldessarini and Tarsey, 1980; Creese and Snyder, 1980 and Jeste and Wyatt, 1981), Parkinson’s disease (Lee et al., 1981 and Rinne, et al., 1981), Huntington’s Chorea (Login et al., 1982) and schizophrenia (Crow et al., 1978; Lee et al., 1978; Lee and Seeman, 1980; and Mackay et al., 1980).


Dopamine Receptor Tardive Dyskinesia Corpus Striatum Synaptosomal Fraction Sodium Borate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baldessarini, RJ and Tarsy, D (1980) Pathophysiologic basis of tardive dyskinesia in long-term effects of neuroleptics., Adv. Biochem. Psychopharmacol. 24, 451–455.Google Scholar
  2. Clement-Cormier, YC and George, RJ (1979) Multiple dopamine binding sites: subcellular localization and bio-chemical characterization. J. Neurochem. 32, 1061–1069.PubMedCrossRefGoogle Scholar
  3. Creese, I and Sibley, DR (1979) Radioligand binding studies: evidence for multiple dopamine receptors. Commun. Psychopharmacol. 3, 385–396.PubMedGoogle Scholar
  4. Creese, I, Usdin, T, and Snyder, SH (1979) Guanine nucleotides distinguish two dopamine receptors. Nature (London) 278, 577–578.CrossRefGoogle Scholar
  5. Creese, I and Snyder, S (1980) Chronic neuroleptic treatment and dopamine receptor regulation. Long-term effects of neuroleptics., Adv. Biochem. Psychopharmacol. 24, 89–94.PubMedGoogle Scholar
  6. Creese, I, Sibley, DR, Leff, S and Hamblin, M (1981) Dopamine receptors: subtypes, localizations and regulation. Fed. Proc. 40, 147–152.PubMedGoogle Scholar
  7. Crow, TJ, Johnstone, EC, Longden, AJ and Owen, F (1978) Dopaminergic mechanisms in schizophrenia: The antipsychotic effects and the disease process. Life Sci. 23, 563–568.PubMedCrossRefGoogle Scholar
  8. Cuatrecasas, P (1970) Protein purification by affinity chromatography., J. Biol. Chem. 245, 3059–3065.PubMedGoogle Scholar
  9. Davis, BJ (1964) Disc electrophoresis-II., Method and application to human serum proteins.,Ann. New York Acad. Sci. 121, 404–427.CrossRefGoogle Scholar
  10. Halaris, AE and Freedman, DS (1977) Vesicular and juxtavesicular serotonin: effects of lysergic acid dithey-lamide and reserpine., J. Pharmacol. Exp. Ther. 203 (3), 575–586.PubMedGoogle Scholar
  11. Hsu, LL and Halaris, AE (1977) Characterization and isolation of dopamine binding protein(s) in rat CNS by affinity chromatography and gel electrophoresis., Proc. Int. Soc. Neurochem. 6, 624Google Scholar
  12. Hsu, LL and Mandell, AJ (1975) Enzymatic formation of tetrahydro-8-carboline from tryptamine and 5-methyl- tetrahydrofolic acid in rat brain fractions: regional and subcellular distribution. J. Neurochem. 24, 631–636PubMedGoogle Scholar
  13. Hsu, LL and Moroi, K (1982) Dopamine binding proteins in synaptical membranes of rat brain., Trans. Amer. Soc. Neurochem. 13 (1), 249Google Scholar
  14. Jeste, DV and Wyatt, RJ (1981) Dogma disputed: Is tardive dyskinesia due to postsynaptic dopamine receptor supersensitivity ? J. Clin. Phychiatry 42 (12), 455–457.Google Scholar
  15. Kebabian, JW and Calne, DB (1979) Multiple receptors for dopamine., Nature(London) 277, 93–96.CrossRefGoogle Scholar
  16. Lee, T and Seeman, P (1980) Elevation of brain neuroleptic /dopamine receptors in schizophrenia. Amer. J. Psychiatry 137, 191–197.Google Scholar
  17. Lee, T, Seeman, P, Tourtellotte, WW, Farley, I and Hornykiewicz, O (1978) Binding of (3H) -neuroleptics and (3H)-apomorphine in schizophrenic brains. Nature (London) 274, 897–900.CrossRefGoogle Scholar
  18. Lee, T, Seeman, P, Hornykiewicz, O, Bilbao, J, Deck, J and Tourtellotte, WW (1981) Parkinson’s disease: low density and presynaptic location of D3 dopamine receptors. Brain Res. 212, 494–498.PubMedCrossRefGoogle Scholar
  19. Login, IS, Coronin, JJ and MacLeod, RM (1982) Neuroleptic malignant syndrome caused by dopamine depleting drugs. Neurology 32, 218–219.PubMedGoogle Scholar
  20. Mackay, AVP, Bird, ED, Iversen, LL, Spokes, EG, Creese, I and Snyder, SH (1980) Dopaminergic abnormalities in post-mortem schizophrenic brain. Adv. Biochem. Psychopharmacol. 24, 325–333.PubMedGoogle Scholar
  21. Nishikori, K, Noshiro, O, Sano, K and Maeno, H (1980) Characterization, solubilization and separation of two distinct dopamine receptors in canine caudate nucleus. J. Biol. Chem. 255 (22), 10909–10915.PubMedGoogle Scholar
  22. Rinne, UK, Lonnberg, P and Koskinen, V (1981) Dopamine receptors in the Parkinsonian brain., J. Neural Transmis. 51, 97–106.CrossRefGoogle Scholar
  23. Sokoloff, P, Martres, MP and Schwartz, JC (1980a) (3H)-Apomorphine labels both dopamine postsynaptic receptors and auto-receptors. Nature 288, 283–286.PubMedCrossRefGoogle Scholar
  24. Sokoloff, P, Martres, MP and Schwartz, JC (1980b) Three classes of dopamine receptor (D-2, D-3, D-4), identified by binding studies with (3H)-apomorphine and (3H)-domperidone. Naunyn-Schmiedelberg’s Arch. Pharmacol. 315, 89–102.CrossRefGoogle Scholar
  25. Titeler, M and Seeman, P (1980) Radioreceptor labeling of pre- and postsynaptic dopamine receptors. Long-term effects of neuroleptics. Adv. Biochem. Psychopharmacol. 24, 159–165.PubMedGoogle Scholar
  26. Whittaker, VP and Barker, LA (1972) The subcellular fractionation of brain tissue with special reference to the preparation of synaptosomes and their component or-ganelles. In: Methods of Neurochemistry (R Fried, ed.) Vol. 2, Marcel Dekker, New YorkGoogle Scholar

Copyright information

© The Humana Press Inc. 1983

Authors and Affiliations

  • Louise L. Hsu
    • 1
  • Kayoko Moroi
    • 1
  • Angelos E. Halaris
    • 2
  1. 1.Department of Psychiatry and Behavioral SciencesThe University of Texas Medical BranchGalvestonUSA
  2. 2.Department of PsychiatryUCLA School of Medicine and Veterans Administration HospitalBrentwood, Los AngelesUSA

Personalised recommendations