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Gas Chromatography for Analysis of Trace Amines in Tissues and Body Fluids

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Neurobiology of the Trace Amines

Abstract

The application of gas chromatography to analysis of trace amines and their metabolites in tissues and body fluids is reviewed. Studies using techniques developed by the authors, i.e. acetylation followed by perfluoroacylation for electron-capture gas chromatography, are described in detail. The advantages and disadvantages of gas chromatography relative to other analytical techniques are outlined, and the possible future directions of gas chromatographic analysis of trace amines are discussed.

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References

  • Argauer R. J. (1968) Rapid procedure for the chloroacetylation of microgram quantities of phenols and detection by electron-capture gas chromatography. Anal. Chem. 40, 122–124.

    Article  PubMed  CAS  Google Scholar 

  • Bailey E. and Barron E. J. (1980) Determination of tranylcypromine in human plasma and urine using high-resolution gas-liquid chromatography with nitrogen sensitive detection. J. Chromatogr. Biomed. Appl. 183, 25–31.

    Article  CAS  Google Scholar 

  • Baker G. B., Calverley D. G., Dewhurst W. G., and Martin I. L. (1979) A sensitive gas chromatographic technique for quantification of urinary tryptamine. Br. J. Pharmac. 67, 469P–470 P.

    CAS  Google Scholar 

  • Baker G. B., Coutts R. T., and Martin I. L. (1981) Analysis of amines in the central nervous system by gas chromatography with electron-capture detection. Progr. Neurobiol. 17, 1–24.

    Article  CAS  Google Scholar 

  • Baker G. B., LeGatt D. F., and Coutts R. T. (1982) A gas chromatographic procedure for quantification of para-tyramine in rat brain. J. Neurosci. Methods 5, 181–188.

    Article  PubMed  CAS  Google Scholar 

  • Blau K., Claxton I. M., Ismahan G., and Sandler M. (1979) Urinary phenylethylamine excretion: gas chromatographic assay with electron-capture detection of the pentafluorobenzoyl derivative. J. Chromatogr. Biomed. Appl. 163, 135–142.

    Article  CAS  Google Scholar 

  • Blau K., King G. S., and Sandler M. (1977) Mass spectrometric and nuclear magnetic resonance confirmation of a 3,3-spirocyclic indole derivative formed from melatonin and related acyl tryptamines. Biomed. Mass Spectrom. 4, 232–236.

    Article  PubMed  CAS  Google Scholar 

  • Borison R. L., Mosnaim A. D., and Sabelli H. C. (1974) Biosynthesis of brain 2-phenylethylamine: influence of decarboxylase inhibitors and D-amphetamine. Life Sci. 15, 1837–1848.

    Article  PubMed  CAS  Google Scholar 

  • Boulton A. A., Juorio A. V., Philips S. R., and Wu P. H. (1975) Some arylalkylamines in rabbit brain. Brain Res. 96, 212–216.

    Article  PubMed  CAS  Google Scholar 

  • Calverley D. G., Baker G. B., McKim H. R., and Dewhurst W. G. (1980) A gas chromatograph technique using electron-capture detection for simultaneous estimation of tryptamine and 5-hydroxytryptamine in biological tissue. Can. J. Neurol. Sci. 7, 237.

    Google Scholar 

  • Cole W. J., Parkhouse J., and Yousef Y. Y. (1977) Application of the extractive alkylation technique to the pentafluorobenzoylation of morphine (a heroin meta-bolite) and surrogates, with special reference to the quantitative determination of plasma morphine levels using mass fragmentography. J. Chromatogr. 136, 409–416.

    Article  PubMed  CAS  Google Scholar 

  • Cone E. J., Risner M. E., and Neidert G. L. (1978) Con-centrations of phenethylamine in dog following single doses and during intravenous self-administration. Res. Commun. Chem. Path. Pharmacol. 22, 211– 232.

    Google Scholar 

  • Coutts R. T., Baker G. B., and Calverley D. G. (1980) A rapid, sensitive method of measuring meta- and para-tyramine levels in urine using electron-capture gas chromatography. Res. Commun. Chem. Path. Pharmacol. 28, 177–184.

    CAS  Google Scholar 

  • Coutts R. T., Baker G. B., LeGatt D. F., Mcintosh G. J., Hopkinson G., and Dewhurst W. G. (1981) Screening for amines of psychiatric interest in urine using gas chromatography with electron-capture detection. Progr. Neuro-Psychopharmacol. 5, 565–568.

    Article  CAS  Google Scholar 

  • Cristofoli W. A., Baker G. B., Coutts R. T., and Benderly A. (1982) Analysis of a monofluorinated analogue of amphetamine in brain tissue using gas chromatography with electron-capture detection. Progr. Neuropsycho-Pharmacol. Biol. Psychiat. 6, 373–376.

    Article  CAS  Google Scholar 

  • Davis B. A. and Boulton A. A. (1981a) Excretion of m-hydroxymandelic acid in human urine. J. Chromatogr. Biomed. Appl. 222, 271–275.

    Article  CAS  Google Scholar 

  • Davis B. A. and Boulton A. A. (1981b) Longitudinal urinary excretion of some “trace” acids in a human male. J. Chromatogr. Biomed. Appl. 222, 161–169.

    Article  CAS  Google Scholar 

  • Davis B. A., Durden D. A., Pun-Li P., and Boulton A. A. (1977) Gas chromatographic procedure for the deter-mination of meta- and para-hydroxyphenylacetic acids. J. Chromatogr. 142, 517–522.

    Article  PubMed  CAS  Google Scholar 

  • Deo P. G. and Howard P. H. (1978) Phosphorylation of alcohols/phenols for gas-liquid chromatographic sep-aration and flame photometric detection. J. Offic. Anal. Chem. 61, 210–213.

    CAS  Google Scholar 

  • Doshi P. S. and Edwards D. J. (1979) Use of 2,6-dinitro- 4-trifluoromethylbenzenesulfonic acid as a novel derivatizing reagent for the analysis of catechol-amines, histamines and related amines by gas chroma-tography with electron-capture detection. J. Chromatogr. 176, 359–366.

    Article  PubMed  CAS  Google Scholar 

  • Doshi P. S. and Edwards D. J. (1981) Effects of L-DOPA on dopamine and norepinephrine concentrations in rat brain assessed by gas chromatography. J. Chromatogr. 210, 505–511.

    Article  PubMed  CAS  Google Scholar 

  • Durden D. A., Philips S. R., and Boulton A. A. (1973) Identification and distribution of B-phenylethyl- amine in the rat. Can. J. Biochem. 51, 995–1002.

    Article  PubMed  CAS  Google Scholar 

  • Edwards D. J. and Blau K. (1972) Analysis of phenylethyl- amines in biological tissues by gas-liquid chromato-graphy with electron-capture detection. Anal. Biochem. 45, 387–402.

    Article  PubMed  CAS  Google Scholar 

  • Edwards D. J. and Blau K. (1973) Phenylethylamine in brain and liver of rats with experimentally induced phenylketonuria-like characteristics. Biochem. J. 132, 95–100.

    PubMed  CAS  Google Scholar 

  • Haeffner L. J., Magen J., and Kowlessar O. D. (1976) The gas-liquid chromatographic separation of selected catecholamines on polyamide A103. J. Chromatogr. 118, 425–428.

    Article  PubMed  CAS  Google Scholar 

  • Jacob K., Falkner C., and Vogt W. (1978) Derivatization method for the high-sensitivity determination of amines and amino acids as dimethylthiophosphinic amides with the alkali flame-ionization detector. J. Chromatogr. 167, 67–75.

    Article  CAS  Google Scholar 

  • Javaid J. I. and Davis J. M. (1981) GLC analysis of phen- ylalkyl primary amines using nitrogen detector. J. Pharm. Sci. 70, 813–815.

    Article  PubMed  CAS  Google Scholar 

  • Karoum F., Nasrallah H., Potkin S., Chuang L., Moyer-Schwing J., Phillips I., and Wyatt R. J. (1979) Mass fragmentography of phenylethylamine, m- and β-tyra- mine and related amines in plasma, cerebrospinal fluid, urine and brain. J. Neurochem. 33, 201–212.

    Article  PubMed  CAS  Google Scholar 

  • LeGatt D. F., Baker G. B., and Coutts R. T. (1981) Simul-taneous extraction and separation of trace amines of biological interest. J. Chromatogr. Biomed. Appl. 225, 301–308.

    Article  CAS  Google Scholar 

  • Martin I. L. and Baker G. B. (1977) A gas-liquid chromatographic method for the estimation of 2-phenyl- ethylamine in rat brain tissue. Biochem. Pharmac. 26, 1513–1516.

    Article  CAS  Google Scholar 

  • McQuade P. L., Juorio A. V., and Boulton A. A. (1981) Estimation of p- and m-isomers of hydroxyphenylacetic acid in mouse brain by a gas chromatographic procedure: their regional distribution and the effects of some drugs. J. Neurochem. 37, 735–739.

    Article  PubMed  CAS  Google Scholar 

  • Midha K. K., McGilveray I. J., and Cooper J. K. (1979) A GLC-ECD assay for simultaneous determination of fen-fluramine and norfenfluramine in human plasma and urine. Can. J. Pharm. Sci. 14, 18–21.

    CAS  Google Scholar 

  • Narasimhachari N. and Friedel R. O. (1981) Quantitation of biologically important primary amines as their isothiocyanate derivatives by gas chromatography using nitrogen detection and validation by selected ion monitoring. Clin. Chim. Acta. 110, 235–243.

    Article  PubMed  CAS  Google Scholar 

  • Oliver J. S., Smith H., and Williams D. J. (1977) The detection, identification and measurement of indole, tryptamine and 2-phenylethylamine in putrefying human tissue. Forensic Sci. 9, 195–203.

    Article  PubMed  CAS  Google Scholar 

  • Oon, M. C. H. and Rodnight R. (1977) A gas chromatographic procedure for determining N,N-dimethyltrypt- amine and N-monomethyltryptamine in urine using a nitrogen detection. Biochem. Med. 18, 410–419.

    Article  PubMed  CAS  Google Scholar 

  • Philips S. R., Durden D. A., and Boulton A. A. (1974a) Identification and distribution of jp-tyramine in the rat. Can. J. Biochem. 52, 336–373.

    Google Scholar 

  • Philips S. R., Durden D. A., and Boulton A. A. (1974b) Identification and distribution of tryptamine in the rat. Can. J. Biochem. 52, 447–451.

    PubMed  CAS  Google Scholar 

  • Reynolds G. P. and Gray D. O. (1976) A method for the estimation of 2-phenylethylamine in human urine by gas chromatography. Clin. Cim. Acta 70, 213–217.

    Article  CAS  Google Scholar 

  • Reynolds G. P. and Gray D. O. (1978) Gas chromatographic detection of N-methyl-2-phenylethylamine: a new component of human urine. J. Chromatogr. Biomed. Appl. 145, 137–140.

    Article  CAS  Google Scholar 

  • Reynolds G. P., Sandler M., Hardy J., and Bradford H. (1980) The determination and distribution of 2-phen-ylethylamine in sheep brain. J. Neurochem. 34, 1123–1125.

    Article  PubMed  CAS  Google Scholar 

  • Sandler M., Ruthven C. R. J., Goodwin B. L., and Reynolds G. P. (1979) Deficient production of tyramine and octopamine in cases of depression. Nature (Lond.) 278, 357–358.

    Article  CAS  Google Scholar 

  • Schweitzer J. W., Friedhoff A. J., and Schwartz R. (1975) Phenethylamine in normal urine: failure to verify high values. Biol. Psychiat. 10, 277–285.

    PubMed  CAS  Google Scholar 

  • Singh J., Cochrane W. P., and Scott J. (1979) Extractive acylation of ethylenethiourea from water. Bull. Environm. Contam. Toxicol. 23, 470–474.

    Google Scholar 

  • Slingsby J. M. and Boulton A. A. (1976) Separation and quantitation of some urinary arylalkylamines. J. Chromatogr. 123, 51–56.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Neurochemical Research Unit, Dept. of Psychiatry and Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada

    Glen B. Baker & Ronald T. Coutts

  2. MRC Neurochemical Pharmacology Unit, Medical Research Council Centre, The Medical School, Hills Road, Cambridge, England

    Ian L. Martin

Authors
  1. Glen B. Baker
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  2. Ronald T. Coutts
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  3. Ian L. Martin
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Editor information

Editors and Affiliations

  1. Psychiatric Research Division, University of Saskatchewan, Saskatoon, Sakastchewan, Canada

    A. A. Boulton

  2. Department of Psychiatry, University of Alberta, Edmonton, Alberta, Canada

    G. B. Baker

  3. Neurochemical Research Unit, Department of Psychiatry, University of Alberta, Edmonton, Alberta, Canada

    W. G. Dewhurst

  4. The Bernhard Baron Memorial Research, Laboratories and Institute of Obstetrics and Gynaecology, Queen Charlotte’s Maternity Hospital, London, England

    M. Sandler

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© 1984 The Humana Press Inc.

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Baker, G.B., Coutts, R.T., Martin, I.L. (1984). Gas Chromatography for Analysis of Trace Amines in Tissues and Body Fluids. In: Boulton, A.A., Baker, G.B., Dewhurst, W.G., Sandler, M. (eds) Neurobiology of the Trace Amines. Humana Press. https://doi.org/10.1007/978-1-4612-5312-9_5

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  • DOI: https://doi.org/10.1007/978-1-4612-5312-9_5

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9781-9

  • Online ISBN: 978-1-4612-5312-9

  • eBook Packages: Springer Book Archive

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