Advertisement

On the Attachment of Ribosomes to Heavy Rough and Light Rough Endoplasmic Reticulum in Eukaryotic Cells

  • Odd Nygård
  • Peter Westermann
Chapter
  • 62 Downloads

Abstract

A 48 hr period of starvation resulted in an almost complete conversion of rough endoplasmic reticulum (ER) membranes to the smooth (S) type. The order of re-appearance of the light rough (LR) and heavy rough (HR) ER fractions after feeding a starved culture was dependent on the nutritional conditions. Inhibition of protein synthesis by cycloheximide resulted in a conversion of HR to S and/or LR membranes. The HR fraction re-appeared within 1/2 hr after removal of the block. RNase treatment of isolated HR membranes resulted in a release of ribosomes sufficient to lower their density causing them to migrate to the position of LR membranes upon re-centrifugation. The migration of LR membranes was unaffected by this treatment. The migratory behaviour of HR membranes was also affected by raising the salt concentration of the gradient buffer from 25mM to 100mM KCl: the membranes appearing in the LR region of the gradient. The results add further support to the suggestion that the HR and LR fractions represent distinct compartments of the rough ER. In addition differences in the mechanisms of association of ribosomes with HR and IR membranes are indicated.

Keywords

Endoplasmic Reticulum Membrane RNase Treatment Discontinuous Sucrose Gradient Polysome Profile Identical Aliquot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blobel, G. and Dobberstein, B. (1975) J. Cell Biol. 67, 835–851.PubMedCrossRefGoogle Scholar
  2. Blobel, G. and Dobberstein, B. (1975) J. Cell Biol. 67, 835–851.PubMedCrossRefGoogle Scholar
  3. Garatun-Tjeldstö, 0., Pryme, I.F., Weltman, J.K. and Dowben, R.M. (1976) J. Cell. Biol. 68, 232–239.Google Scholar
  4. Grubman, M.J., Moyer, S.A., Banerjee, A.K. and Ehrenfeld, E. (1975) J. Cell Biol. 74, 43–57.CrossRefGoogle Scholar
  5. Lodish, H.F. and Small, B. (1975) J. Cell Biol. 65, 51–64.PubMedCrossRefGoogle Scholar
  6. Pryme, I.F. (1974) FEBS Lett. 48, 200–203.PubMedCrossRefGoogle Scholar
  7. Pryme, I.F., Svardal, A.M. and Skorve, J. (1981) Mol. Cell. Biochem. 34, 177–183.PubMedCrossRefGoogle Scholar
  8. Pryme, I.F., Svardal, A.M., Skorve, J. and Lillehaag, J.R. (1982) In: Methodological Surveys in Biochemistry - Cancer Cell Organelles (E. Reia, G.M.W. Cook and D.J. Morre, eds.) vol. 11, pp 293–293,Horwood, Chichester, U.K.Google Scholar
  9. Sabatini, D.D. and Kreibich, G. (1976) In: The Enzymes of Biological Membranes (Martooosi, A. ed.) vol. 2, pp 531–579, Plenum, U.S.A.Google Scholar
  10. Shore, G.C. and TaVa, J.R. (1977a) J. Cell Biol. 72, 714–725.PubMedCrossRefGoogle Scholar
  11. Shore, G.C. and Tata, J.R. (1977b) J. Cell Biol. 72, 726–743.PubMedCrossRefGoogle Scholar
  12. Svardal, A.M. and Pryme, I.F. (1978) Anal. Biochem. 89, 332–336.PubMedCrossRefGoogle Scholar
  13. Svardal, A.M. and Pryme, I.F. (1930a) Subcell. Biochem. 7, 117–170.Google Scholar
  14. Svardal, A.M. and Pryme, I.F. (1980b) Mol. Cell. Biochem. 29, 159–171.PubMedCrossRefGoogle Scholar
  15. Svardal, A.M. and Pryine, I.F. (1980c) Mol. Biol. Rep. 6, 105–110.PubMedCrossRefGoogle Scholar
  16. Svardal, A.M., Pryme, I.F. and Dalen, H. (1981) Mol. Cell. Biochem. 34, 165–175.PubMedCrossRefGoogle Scholar
  17. Tata, J.R. (1971) Subcell. Biochem. 1, 83–39.Google Scholar
  18. Tata, J.R. (1973) In: Reproductive Endocrinology, 6th Symposium, Karolinska Symposia on Research Methods (E. Diczfalusy, ed.) pp 192–224, Karolinska Institute, Stockholm.Google Scholar
  19. Wirth, D.F., Katz, F., Small, R. and Lodish, H.F. (1977) Cell 10, 253–263.PubMedCrossRefGoogle Scholar

Copyright information

© The Human Press Inc. 1983

Authors and Affiliations

  • Odd Nygård
    • 1
  • Peter Westermann
    • 2
  1. 1.The Wenner-Gren InstituteUniversity of StockholmStockholmSweden
  2. 2.Central Institute of Molecular BiologyAcademy of Sciences of GDRBerlin-BuchGermany

Personalised recommendations