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Hormonal Control of Adipose Tissue Lipolysis by Phosphorylation/Dephosphorylation of Hormone-Sensitive Lipase

  • Odd Nygård
  • Peter Westermann
Chapter
  • 66 Downloads

Abstract

The quantitatively most important energy substrate in mammals are free fatty acids (FFA), stored as triacylglycerol in the adipocytes. Hormones and the sympathetic nervous system regulate the mobilization of FFA from the adipose tissue, and the rate of this process mainly reflects the hydrolysis of the intracellular triacylglycerols, in the process of adipose tissue lipolysis. Fast-acting lipolytic hormones increase, and insulin inhibits this lipolysis by controlling the activity of the hormone-sensitive lipase (HSL), the rate-limiting enzyme. The lipase catalyzes hydrolysis of the first ester bond of the triacylglycerol substrate, which is the rate-limiting step of fat breakdown, and also the degradation of the 1,2 (2,3)-diacylglycerols to 2-monoacylglycerols. The final hydrolysis of the monoacylglycerols to FFA and glycerol is catalyzed by a separate monoacylglycerol lipase (15).

Keywords

Hormonal Control Reversible Phosphorylation Cholesterol Ester Hydrolase Adipose Tissue Lipolysis Monoacylglycerol Lipase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Belfrage, P. (1983): Hormonal control of lipid degradation. In: New Perspectives in Adipose Tissue Structure, Function and Development, edited by A. Cryer and R.L.R. Van. Butterworths, London. In press.Google Scholar
  2. 2.
    Belfrage. P., Fredrikson, G., Nilsson, N.Ö. and Strålfors, P. (1980): Phosporylation of hormone-sensitive lipase in intact rat adipocytes. FEBS Lett., 111: 120–124.PubMedCrossRefGoogle Scholar
  3. 3.
    Belfrage, P., Fredrikson, G., Nilsson, N.Ö. and Strålfors, P. (1981): Regulation of adipose tissue lipolysis by phosphorylation of hormone-sensitive lipase. Int. J. Obesity, 5: 635–641.Google Scholar
  4. 4.
    Belfrage, P., Fredrikson, G., Strålfors, P. and Tornqvist, H. (1983): Adipose tissue lipases. In: Lipases, edited by B. Borgström and H. Brockman. Elsevier/North Holland, Amsterdam. In press.Google Scholar
  5. 5.
    Cook, K.G. and Yeaman, S.J.; Stålfors, P., Fredrikson, G. and Belfrage, P. (1982): Direct evidence that adrenal cortex cholesterol ester hydrolase is the same enzyme as adipose tissue hormone-sensitive lipase. Eur. J. Biochem., 125: 245–249.PubMedCrossRefGoogle Scholar
  6. 6.
    Cook, K.G., Colbran, R.J., Snee, J. and Yeaman, S.J. (1983): Cytosolic cholesterol ester hydrolase from bovine corpus luteum: its purification, identification and relationship to hormone-sensitive lipase. Biochim. Biophys. Acta. In press.Google Scholar
  7. 7.
    Fredrikson, G., Strålfors, P., Nilsson, N.Ö. and Belfrage, P. (1981): Hormone-sensitive lipase of rat adipose tissue: purification and some properties. J. Biol. Chem., 256: 6311–6320.PubMedGoogle Scholar
  8. 8.
    Ingebritsen, T.S. and Cohen, P. (1983): The protein phosphatases involved in cellular regulation. 1. Classification and substrate specificities. Eur. J. Biochem., 132: 255–261.PubMedCrossRefGoogle Scholar
  9. 9.
    Nilsson, N.Ö. (1981): Studies on the short-term regulation of lipolysis in rat adipocytes with special regard to the anti-lipolytic effect of insulin. Thesis, University of Lund, Sweden. ISBN 91–7222–402–9.Google Scholar
  10. 10.
    Nilsson, N.Ö., Strålfors, P., Fredrikson, G. and Belfrage, P. (1980): Effects of noradrenaline and insulin on phosphorylation of hormone-sensitive lipase and on lipolysis in intact rat adipcytes. FEBS Lett., 111: 125–130.PubMedCrossRefGoogle Scholar
  11. 11.
    Nilsson, N.Ö. and Belfrage, P. (1981): Continuous measurement of free fatty acid release from intact adipocytes by pH-stat titration. In: Meth. Enzymol., vol. 72, edited by J.M. Lowenstein, pp. 319–325. Acad. Press, New York.Google Scholar
  12. 12.
    Steinberg. D. and Huttunen, J.K. (1972): The role of cyclic AMP in activation of hormone-sensitive lipase of adipose tissue. Adv. Cyclic Nucl. Res., 1: 47–62.Google Scholar
  13. 13.
    Strålfors, P. (1983): Regulation of hormone-sensitive lipase through reversible phosphorylation. Role of cyclic AMP-dependent protein kinase. Thesis. University of Lund, Sweden. ISBN 91–722–627–7.Google Scholar
  14. 14.
    Strålfors, P. and Belfrage, P. (1983): Reversible phosphorylation of hormone-sensitive lipase/cholesterol ester hydrolase in the hormonal control of adipose tissue lipolysis and of adrenal steroidogenesis. In: Molecular Aspects of Cellular Regulation, vol. 3. Recently Discovered systems of Enzyme Regulation by Reversible Phosphorylation. Part 2., edited by P. Cohen. Elsevier/North Holland, Amsterdam. In press.Google Scholar
  15. 15.
    Tornqvist, H. and Belfrage, P. (1976): Purification and some properties of a monoacylglycerol-hydrolyzing enzyme of rat adipose tissue. J. Biol. Chem., 251: 813–819.PubMedGoogle Scholar

Copyright information

© The Human Press Inc. 1983

Authors and Affiliations

  • Odd Nygård
    • 1
  • Peter Westermann
    • 2
  1. 1.The Wenner-Gren InstituteUniversity of StockholmStockholmSweden
  2. 2.Central Institute of Molecular BiologyAcademy of Sciences of GDRBerlin-BuchGermany

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