Initiation Factors Which Recognize mRNA

  • Odd Nygård
  • Peter Westermann


A number of discoveries in the last few years have added new and clarifying information to the interpretation of events in eukaryotic protein synthesis initiation (Jagus et al., 1981). Of those discoveries, perhaps the most notable are those that relate to the binding of mRNA to 40S ribosomal subunits as this area had generated only limited information on the mRNA recognition process and often there appeared to be conflicting reports on the specific properties of given initiation factors, especially eIF-3 and eIF-4B. Several independent lines of research have subsequently generated substantial clarification. The first line was the development of an assay by Shatkin and co-workers to identify those proteins which appeared to specifically recognize the m7GDP cap structure of eukaryotic rRNAs (Sonenberg & Shatkin, 1977; Sonenberg et al., 1978). A separate methodology developed by Marilyn Kozak has probed the ATP requirement for the binding of mRNA to 40S subunits. Her conclusion from these studies was that there are two ATP-dependent steps, one in which an area of the mRNA near the cap structure is bound to the 40S ribosomal subunit and a second step where the mRNA is “scanned” to locate the initiating AUG codon (Kozak, 1978; Kozak, 1980a; Kozak, 1980b).


Initiation Factor Magnesium Acetate mRNA Binding Preinitiation Complex Globin mRNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Human Press Inc. 1983

Authors and Affiliations

  • Odd Nygård
    • 1
  • Peter Westermann
    • 2
  1. 1.The Wenner-Gren InstituteUniversity of StockholmStockholmSweden
  2. 2.Central Institute of Molecular BiologyAcademy of Sciences of GDRBerlin-BuchGermany

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