The Allosteric Behaviour of the Elongation Factor EF-Tu

  • Odd Nygård
  • Peter Westermann


The E. coli factor EF-Tu is a multifunctional protein that interacts with guanine nucleotides, tRNA, the elongation factor EF-Ts, the ribosome, the antibiotics kirromycin and pulvomycin, and probably bacteriophage RNA. It thus fulfils an essential function in protein synthesis (Miller & Weissbach, 1977; Kaziro, 1978) and phage RNA replication (Blumenthal & Carmichael, 1979). It is an abundant cytoplasmic protein that, depending on bacterial growth conditions, can amount from five to ten percent of the total intracellular protein (Furano, 1975; Van der Meide et al., 1983). Its amino acid sequence (393 residues) has been determined (Arai et al.,1980; Jones et al., 1980) and considerable progress has been made in the elucidation of its three-dimensional structure (Kabsch et al., 1977; Jurnak et al., 1977; Rubin et al. 1981; Clark et al., 1982).


tRNA Binding Uncharged tRNA tRNA Binding Site Allosteric Behavior Polypeptide Chain Elongation 
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Copyright information

© The Human Press Inc. 1983

Authors and Affiliations

  • Odd Nygård
    • 1
  • Peter Westermann
    • 2
  1. 1.The Wenner-Gren InstituteUniversity of StockholmStockholmSweden
  2. 2.Central Institute of Molecular BiologyAcademy of Sciences of GDRBerlin-BuchGermany

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