Abstract
An in vitro mouse skeletal muscle preparation, the teased fibre bundle, has been investigated as a means of study of protein turnover. The repsonse of this preparation to nutrient supply is reported and compared with the results of other in vitro skeletal muscle preparations from rodents.
Using the preparation, we have observed, in agreement with other studies, that the rate of protein degradation in mouse dystrophic muscle is significantly elevated. The effects of various protease inhibitors have been tested in this system and one, leupeptin, showed a significant and consistent effect in decreasing protein degradation in normal and dystrophic tissue.
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Butcher, L., Tomkins, J.K. (1983). Skeletal Muscle Fibre Bundles for the Study of Protein Turnover in Normal and Dystrophic Mouse Tissue. In: Kidman, A.D., Tomkins, J.K., Morris, C.A., Cooper, N.A. (eds) Molecular Pathology of Nerve and Muscle. Experimental and Clinical Neuroscience. Humana Press. https://doi.org/10.1007/978-1-4612-5308-2_24
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DOI: https://doi.org/10.1007/978-1-4612-5308-2_24
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