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Combined Gas Chromatography and Electroantennogram Recording of Insect Olfactory Responses

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Techniques in Pheromone Research

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

Electroantennogram (EAG)3 recording of insect olfactory responses has been a great asset as a method of bioassay for chemical identifications of pheromones and for the development of synthetic attractants (Roelofs, Chapter 5). When EAG is used separately from the purification method, e.g., gas chromatography (GC), it is necessary to isolate and transfer small amounts (ng) of purified components to the EAG preparation. This does not take full advantage of the GC resolution capabilities and it may result in a loss of essential minor pheromone components. It is also time consuming.

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Author information

Authors and Affiliations

  1. Research Station, Agriculture Canada, Lethbridge, Alberta, Canada

    Dean L. Struble

  2. Swiss Federal Research Station, CH-8820, Wädenswil, Switzerland

    Heinrich Arn

Authors
  1. Dean L. Struble
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  2. Heinrich Arn
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Editor information

Editors and Affiliations

  1. Harbor Branch Foundation, 33450, Fort Pierce, Florida, USA

    Hans E. Hummel

  2. Department of Entomology, University of California, 92521, Riverside, California, USA

    Thomas A. Miller

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© 1984 Springer-Verlag New York Inc.

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Struble, D.L., Arn, H. (1984). Combined Gas Chromatography and Electroantennogram Recording of Insect Olfactory Responses. In: Hummel, H.E., Miller, T.A. (eds) Techniques in Pheromone Research. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5220-7_6

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  • DOI: https://doi.org/10.1007/978-1-4612-5220-7_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9743-7

  • Online ISBN: 978-1-4612-5220-7

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