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Techniques for Behavioral Bioassays

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Techniques in Pheromone Research

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

Insect pheromone research involves tremendous effort and exacting techniques, not only for isolating and identifying chemicals, covered in earlier and later chapters in this volume, but also in recognizing which chemicals are behaviorally active. Tests to demonstrate the behavioral activity of a compound are essential to proving that a compound is a pheromone component, i.e., that it is used in intraspecific communication. In addition, behavioral tests, or bioassays, of chemicals’ effects on receiving individuals can identify the type of response they elicit. Therefore, well-designed bioassays can be invaluable for deducing the communicative function (alarm, aggregation, sexual communication, etc.) of a chemical identified from an insect. They can also give information as to the mechanisms that are used by responding insects to move toward or away from the chemical source.

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References

  • Baker TC, CardĂ©T (1979) Analysis of pheromone-mediated behavior in male Grapholitha molesta, the oriental fruit moth (Lepidoptera:Tortricidae). Environ Entomol 8: 956–968.

    CAS  Google Scholar 

  • Sex pheromone dosage and blend specificity fo response by oriental fruit moth males. Ent Exp Appl30:269–279.

    Article  CAS  Google Scholar 

  • Baker TC, CardĂ©, Roelofs WL (1976) Behavioral responses of male Argyrotaenia velutinana ( Lepidoptera: Tortricidae) to components of its sex pheromone. J Chem Ecol 2: 333–352.

    Article  CAS  Google Scholar 

  • Bartell RJ, Lawrence LA (1977) Reduction of responsiveness of male apple moths, Epiphyas postvittana to sex pheromone following pulsed pheromonal exposure. Physiol Entomol 2: 1–6.

    Article  CAS  Google Scholar 

  • Bartell RJ, Shorey HH (1969) A quantitative bioassay for the sex pheromone of Epiphyas postvittana ( Lepidoptera) and factors limiting male responsiveness. J Insect Physiol 15: 33–40.

    Article  CAS  Google Scholar 

  • Bell WJ, Kramer E (1980) Sex pheromone stimulated orientation responses by the American cockroach on a servosphere apparatus. J Chem Ecol 6: 287–295.

    Article  Google Scholar 

  • Bell WJ, Tobin T (1981) Orientation to sex pheromone in the American cockroach: Analysis of chemo-orientation mechanisms. J Insect Physiol 27: 501–508.

    Article  Google Scholar 

  • Bell WJ, Tobin TR (1982) Chemo-orientation. Biol Rev 57: 219–260.

    Article  Google Scholar 

  • CardĂ© T, Comeau A, Baker TC, Roelofs WL (1975) Moth mating periodicity: Temperature regulates the circadian gate. Experientia 31: 46–48.

    Article  PubMed  Google Scholar 

  • CardĂ©T, Hagaman TE (1979) Behavioral responses of the gypsy moth in a wind tunnel to air-borne enantiomers of disparlure. Environ Entomol 8: 475–484.

    Google Scholar 

  • CardĂ©T, Hagaman TE (1983) Influence of ambient and thoracic temperatures upon sexual behaviour of the gypsy moth, Lymantria dispar. Physiol Entomol 8: 7–14.

    Article  Google Scholar 

  • Corbet PS (1966) The role of rhythms in insect behaviour. In: Insect Behaviour. Haskell PT (ed), Symp R Ent Soc Lond, Vol 3, pp 13–28.

    Google Scholar 

  • Daterman GE (1972) Laboratory bioassay for sex pheromone of the European pine shoot moth, Rhyacionia buoliana. Ann Entomol Soc Am 65: 119–123.

    Google Scholar 

  • Fitzgerald TD, Gallagher EM (1976) A chemical trail factor from the silk of the Eastern tent caterpillar Malacosoma americanum ( Lepidoptera: Lasiocampi- dae). J Chem Ecol 2: 187–193.

    Article  CAS  Google Scholar 

  • Fitzgerald TD, Edgerly JS (1979) Specificity of trail markers of forest and Eastern tent caterpillars. J Chem Ecol 5: 565–574.

    Article  Google Scholar 

  • Fraenkel GS, Gunn DL (1940) The Orientation of Animals. Revised edition ( 1961 ) Dover, New York.

    Google Scholar 

  • Hawkins WA (1978) Effects of sex pheromone on locomotion in the male American cockroach. J Chem Ecol 4: 149–160.

    Article  CAS  Google Scholar 

  • Kennedy JS (1977) Behaviorally discriminating assays of attractants and repellents. In: Chemical Control of Insect Behavior: Theory and Application. Shorey HH, McKelvey J J (eds), Wiley, New York, pp 215–229.

    Google Scholar 

  • Kennedy JS (1978) The concepts of olfactory arrestment and attraction. Physiol Entomol 3: 91–98.

    Article  Google Scholar 

  • Kennedy JS, Marsh D (1974) Pheromone-regulated anemotaxis in flying moths. Science 184: 999–1001.

    Article  PubMed  CAS  Google Scholar 

  • Kramer E (1975) Orientation of the male silkmoth to the sex attractant bombykol. Olfaction and Taste V. Dento D, Coghlan JP (eds), Academic Press, New York, pp 329–335.

    Google Scholar 

  • Kuenen LPS, Baker TC (1981) Habituation versus sensory adaptation as the cause of reduced attraction following pulsed and constant sex pheromone pre-exposure in Trichoplusia ni. J Insect Physiol 27: 721–726.

    Article  CAS  Google Scholar 

  • Linn CE Jr, Gaston LK (1981) Behavioral function of the components and the blend of the sex pheromone of the cabbage looper, Trichoplusia ni. Environ Entomol 10: 751–755.

    CAS  Google Scholar 

  • Miller JR, Baker TC, Carde RT, Roelofs WL (1976) Reinvestigation of oak leaf roller sex pheromone components and the hypothesis that they vary with diet. Science 192: 140–143.

    Article  PubMed  CAS  Google Scholar 

  • Miller JR, Roelofs WL (1978a) Sustained-flight tunnel for measuring insect re¬sponses to wind-borne sex pheromones. J Chem Ecol 4: 187–198.

    Article  Google Scholar 

  • Miller JR, Roelofs WL (1978b) Gypsy moth responses to pheromone enantiomers as evaluated in a sustained-flight tunnel. Environ Entomol 7: 42–44.

    CAS  Google Scholar 

  • Montgomery MES, Nault LR (1977a) Comparative response of aphids to the alarm pheromone ( E)-β-farnesene. Ent Exp Appl 22: 236–242.

    Article  CAS  Google Scholar 

  • Montgomery MES, Nault LR (1977b) Aphid alarm pheromones: Dispersion of Hyadaphis erysimio and Myzus persicae. Ann Entomol Soc Am 70: 669–672.

    Google Scholar 

  • Palaniswamy P, Underhill EW, Steck WF, Chisholm MD (1983) Responses of male redbacked cutworm, Euxoa ochrogaster ( Lepidoptera: Noctuidae) to sex pheromone components in a flight tunnel. Environ Entomol 12: 748–752.

    CAS  Google Scholar 

  • Payne TL, Hart ER, Edson LJ, McCarty FA, Billings PM, Coster JE (1976) Olfactometer for assay of behavioral chemicals for the Southern pine beetle, Dendroctonus frontalis ( Coleoptera: Scolytidae). J Chem Ecol 2: 411–419.

    Article  Google Scholar 

  • Persoons CJ (1977) Structure elucidation of some insect pheromones: A contribution to the development of selective pest control agents. PhD thesis, Agricultural University, Wageningen, The Netherlands.

    Google Scholar 

  • Roelofs WL, Hill AS, CardĂ© T, Baker TC (1974) Two sex pheromone components of the tobacco budworm moth, Heliothis virescens. Life Sci 14: 1555–1561.

    Article  PubMed  CAS  Google Scholar 

  • Rust MK, Burk T, Bell WJ (1976) Pheromone stimulated locomotory and orientation responses in the American cockroach. Anim Behav 24: 52–67.

    Article  Google Scholar 

  • Shorey HH (1970) Sex pheromones of Lepidoptera. In: Control of Insect Behavior by Natural Products. Wood DL, Silverstein RM, Nakajima M (eds), Academic Press, New York, pp 249–284.

    Google Scholar 

  • Shorey HH, Gaston LK (1964) Sex pheromones of noctuid moths III. Inhibition of male responses to the sex pheromone in Trichoplusia ni ( Lepidoptera: Noctuidae). Ann Entomol Soc Am 57: 775–779.

    Google Scholar 

  • Sower LL, Vick KW, Long JS (1973) Isolation and preliminary biological studies of the female-produced sex pheromone of Sitotroga cerealella ( Lepidoptera: Gelechiidae). Ann Ent Soc Am 66: 184–187.

    CAS  Google Scholar 

  • Tobin TR, Seelinger G, Bell WJ (1981) Behavioral responses of male Periplaneta americana to periplanone B, a synthetic component of the female sex pheromone. J Chem Ecol 7 : 969–979.

    Article  Google Scholar 

  • Vetter RS, Baker TC (1982) Behavioral responses of male Heliothis virescens in a sustained-flight tunnel to combinations of the 7 compounds identified from the female sex pheromone gland. J Chem Ecol 9: 747–759.

    Article  Google Scholar 

  • Vick KW, Burkholder WE, Gorman JE (1970) Interspecific response to sex pheromones of Trogoderma species ( Coleoptera: Dermestidae). Ann Entomol Soc Am 63: 379–381.

    Google Scholar 

  • Van Vorhis Key SE, Baker TC (1981) Effects of gaster extract trail concentration on the trail following behaviour of the Argentine ant, Iridomyrmex humilis ( Mayr ). J Insect Physiol 27: 363–370.

    Article  Google Scholar 

  • Van Vorhis Key SE, Baker TC (1982) Specificity of laboratory trail following by the Argentine ant, ridomyrmex humilis (Mayr), to (Z)-9-hexadecenal, analogs, and gaster extracts. J Chem Ecol 8: 1057–1063.

    Article  Google Scholar 

  • Von Keyserlingk H (1982) The measurement of attractant or repellent effects of chemicals on walking insects. Med Fac Landbouww Rijksuniv Gent 47: 547–555.

    Google Scholar 

  • Wood DL, Bushing RW (1963) The olfactory response of Ips confusus (LeC.) ( Coleoptera: Scolytidae) to the secondary attraction in the laboratory. Can Entomol 95: 1066–1078.

    Article  Google Scholar 

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© 1984 Springer-Verlag New York Inc.

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Baker, T.C., Cardé, R.T. (1984). Techniques for Behavioral Bioassays. In: Hummel, H.E., Miller, T.A. (eds) Techniques in Pheromone Research. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5220-7_2

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  • DOI: https://doi.org/10.1007/978-1-4612-5220-7_2

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9743-7

  • Online ISBN: 978-1-4612-5220-7

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