Is Phosphatidylinositol Involved in the Release of Free Fatty Acids in Cerebral Ischemia?

Sun, G. Y.; Tang, W.; Huang, S. F-L.; Foudin, L.

doi:10.1007/978-1-4612-5184-2_33

Is Phosphatidylinositol Involved in the Release of Free Fatty Acids in Cerebral Ischemia?

G. Y. Sun⁴,
W. Tang⁴,
S. F-L. Huang⁴ &
…
L. Foudin⁴

Chapter

48 Accesses
4 Citations

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 6))

Summary

Since acyl groups of phosphatidylinositol (PI) are enriched in stearic (18:0) and arachidonic (20:4) acids, and these fatty acids are preferentially released during cerebral ischemia, a metabolic relationship between PI and the free fatty acid (FFA) release is suggested. However, several lines of evidence have pointed against a direct involvement of the PI in this event. In our studies, ischemia elicited an increase in the level of diacylglycerols (DG) enriched in 18:0 and 20:4, but the level of PI in rat brain synaptosomes was not changed. Furthermore, active hydrolysis of PI by phosphodiesterase could not be demonstrated in synaptosomes under physiologically feasible conditions, although incubation of the same fraction resulted in a two-fold increase in DG. When rats pre-labeled with ³²P_i were subjected to post-decapitative ischemic treatment, radioactivity of phosphatidylinositol 4,5-bisphosphate (PI P₂) and phosphatidate (PA) in synaptosomes was decreased, but that of PI increased. These results suggest that an increase in enzymic degradation of these lipids coupled with hydrolysis of DG by DG lipase may account for the increase in DG and FFA enriched in 18:0 and 20:4 during the early phase of cerebral ischemia.

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Sinclair Comparative Medicine Research Farm and Biochemistry Department, University of Missouri, Columbia, MO, 65201, USA
G. Y. Sun, W. Tang, S. F-L. Huang & L. Foudin

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G. Y. Sun
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W. Tang
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S. F-L. Huang
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L. Foudin
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Department of Biochemistry, University of Texas Health Science Center, Dallas, Texas, USA
John E. Bleasdale
Department of Biochemical and Biophysical Sciences, University of Houston, Houston, Texas, USA
Joseph Eichberg
Ralph Lowell Laboratories, McLean Hospital, Harvard Medical School, Belmont, Massachusetts, USA
George Hauser

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Sun, G.Y., Tang, W., Huang, S.FL., Foudin, L. (1985). Is Phosphatidylinositol Involved in the Release of Free Fatty Acids in Cerebral Ischemia?. In: Bleasdale, J.E., Eichberg, J., Hauser, G. (eds) Inositol and Phosphoinositides. Experimental Biology and Medicine, vol 6. Humana Press. https://doi.org/10.1007/978-1-4612-5184-2_33

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DOI: https://doi.org/10.1007/978-1-4612-5184-2_33
Publisher Name: Humana Press
Print ISBN: 978-1-4612-9602-7
Online ISBN: 978-1-4612-5184-2
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