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Tailor-Made Agarose-Based Reactive Beads for Hemoperfusion and Plasma Perfusion

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Microencapsulation and Artificial Cells

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Abstract

Composite beads of approximately 1mm diameter, made of cross-linked agarose and containing Fuller’s Earth or zirconium oxide powders, were prepared and used in extracorporeal systems for blood detoxification. The former was used for the removal of Paraquat, while the latter was used to remove inorganic phosphate from hyperphosphatemic animals with or without acute renal failure. The high surface area of the powder, combined with the low resistance to diffusion in the cross-linked agarose matrix, are highly advantageous. The crosslinking provides high mechanical strength, heat stability, prolonged shelf life, good blood flow characteristics, and prevents the release of fine particles into the blood.

Crosslinked agarose beads of 1 mm diameter, containing chemically-bound heparin were also prepared, and used as a model for direct contact removal of LDL-cholesterol from the blood of familial hypercholesterolemic patients by hemoperfusion. The high capacity of these beads (over 5 mg LDL/mL beads) indicates that this clinical modality can replace the highly expensive plasmapheresis procedure presently used.

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References

  1. Yatzidis, H. (1964), Proc. Eur. Dial. Transpl. Assoc 1, 83.

    Google Scholar 

  2. Chang, T. M. S. (1964), Science 146, 524.

    Article  PubMed  CAS  Google Scholar 

  3. Chang, T. M. S. (1977), in Artificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds., Macmillan, London, pp. 164–177.

    Google Scholar 

  4. Fennimore, J., Kolthamer, J. C., and Lang, S. M. (1977), in Artificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds. Macmillan, London, pp. 148–157.

    Google Scholar 

  5. Okonek, S. (1980), in Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 219–228.

    Google Scholar 

  6. Sideman, S., Mor, L., Rousseau, I., Brandes, J. M., and Ben-Arie, D. (1977), in Artificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds., Macmillan, London, pp. 413–424.

    Google Scholar 

  7. Cooney, D. D., Infantolino, W., and Kane, R. (1979), Biomat. Med. Dev. Art. Org 7, 443.

    CAS  Google Scholar 

  8. Voland, H. W., Schirop, T., Barckow, D., and Ibe, K. ( 1980, in Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 319–326.

    Google Scholar 

  9. Malchesky, P. S,. Varne, W., Piatkiewicz, S. and Nose, Y. (1977), Trans. Am. Soc. Artif. Intern. Organs 23, 659.

    PubMed  CAS  Google Scholar 

  10. Malchesky, P. S., Asanuma A., Smith, J., Zawicki, I., and Nose, Y. (1980), in: Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 111–119.

    Google Scholar 

  11. Randerson, D. H., Gurland, H. J., Schmidt, B., Farrell, P. C., Hone, P. W. E., Stokoe, C., Zuber, A., Blogg, A., Fatheh-Moghadam, A., Marschner, I., and Kopcke, W. (1982), in: Hemoperfusion, Bonomini, V., and Chang, T. M. S., eds., Karger, Basel. Contr. Nephrol 29, 53.

    Google Scholar 

  12. Tangerman, A., Snel., P., Verkooyen, Ad. H. M., Hectors, M. P. C., and von Tongeren, J. H. M. (1982), Artif. Organs 4, 302.

    Article  Google Scholar 

  13. van Berlo, A., Verkooyen, A., and Tangerman, A. (1982), Int. J. Artif. Organs, 5, 181.

    PubMed  Google Scholar 

  14. Chang, T. M. S. (1976), in Methods in Enzymology vol, 44, Mosbach, K., ed., Academic Press, New York, p. 676.

    Google Scholar 

  15. Chang, T. M. S. (1977), in Biomedical Application of Immobilized Enzymes and Proteins, Chang, T. M. S., ed., vol. I, Plenum Press, New York, p. 147.

    Google Scholar 

  16. Shu, C. D., and Chang, T. M. S. (1980), Int. J. Artif. Organs 3, 287.

    PubMed  CAS  Google Scholar 

  17. Grunwald, J., and Chang, T. M. S. (1980), in Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY pp. 389–394.

    Google Scholar 

  18. Chang, T. M. S. (1980), Trans. Am. Soc. Artif. Int. Organs 26, 546.

    CAS  Google Scholar 

  19. Arnott, S., Fulmer, A., Scott, W. E., Dea, I. C. M., Moorhouse, R., and Rees, D. A. (1974), J. Mol. Biol 90, 269.

    Article  PubMed  CAS  Google Scholar 

  20. Porath, J., Jonson, J. C., and Laas, I. (1971), J. Chromatogr. 60, 167.

    Article  CAS  Google Scholar 

  21. Tabak, A., Lotan, N., Sideman, S., and Taitelman, U. (1983), Artif. Organs, 7, 146.

    Article  Google Scholar 

  22. Sideman, S., Tatelman, U., Tabak, A., Lotan, N., Mihich, M., and Lupovich, S. (1982), Vet. Hum. Tox,24–5 43.

    Google Scholar 

  23. Laas, T. (1975), J. Chromatogr. 111, 373.

    Article  PubMed  CAS  Google Scholar 

  24. deKoning, H. W. M., Chamuleau, R. A. F. M., and Bantjes, A. (1982), Artif. Organs 6, 280.

    Article  CAS  Google Scholar 

  25. Hughes, R. D., Dunlop, E. H., Davis, M., and Silk, D. B. A. (1977), Biomat. Med. Dev. Artif. Organs 5, 205.

    CAS  Google Scholar 

  26. Lupien, P. J., Moorjani, S., and Awad, J. (1976), Lancet ii, 1261.

    Google Scholar 

  27. Moorjani, S., Lupien, P. J., and Awad, J. (1977), Clin. Chim. Acta 77, 21.

    Article  PubMed  CAS  Google Scholar 

  28. Brunner, G., and Loesgen, H. (1977), in Artificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds., Macmillan, London, pp. 388–394.

    Google Scholar 

  29. Brunner, G., Harstick, K., and Holloway, C. J. (1980), in Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 37–44.

    Google Scholar 

  30. Xu, C. X., Tang, X. J., Niu, Z., and Li, Z. N. (1981), Int. J. Artif. Organs 4, 200.

    PubMed  CAS  Google Scholar 

  31. Jonsson, S., and Hakansson, L. (1981), Artif. Organs 5 (Abstr.), 34.

    Google Scholar 

  32. Jonsson, S., Hakansson, L., and Nylen, U. (1981), Artif. Organs 5 (Abstr.)

    Google Scholar 

  33. Burgstaler, E. A., Pineda, A. A., and Ellefson, R. D. (1980), Mayo Clin. Proc 55, 180.

    PubMed  CAS  Google Scholar 

  34. Schmer, G., Rastelli, L., Dennis, M. B., Hershon, K., Young, J., and Chait, A. (1981), Trans. Am. Soc. Artif. Organs 27, 547.

    CAS  Google Scholar 

  35. Sideman, S., Lotan, N., Tabak, A., Mor, L., Duveborg, L., Bleiberg, B., and Brook, J., to be published (1983).

    Google Scholar 

  36. Stoffel, W., and Demant, T. (1981), Proc. Natl. Acad. Sci. US 78, 611.

    Article  CAS  Google Scholar 

  37. Stoffel, W., Borberg, W., and Greve, V. (1981), Lancet ii, 1005.

    Article  Google Scholar 

  38. Sideman, S., Hoffer, E., and Brunner, G. (1980), Technion R & D Found. Ltd., Proj. 071–182, Rept. No. 72 to MEP Found, Women’s Division, ATS, NY, USA.

    Google Scholar 

  39. Sideman, S., Manor, D., Hoffer, E., Tabak, A., Lotan, N., Mordochovich, D., and Brunner, G. (1983), Life Support Syst. 1, 113.

    PubMed  CAS  Google Scholar 

  40. Nilsson, K., and Mosbach, K. (1980), Eur. J. Biochem 112, 397.

    Article  PubMed  CAS  Google Scholar 

  41. Clerk, D. G. (1971), Brit. J. Ind. Med 28, 186.

    Google Scholar 

  42. Amphlett, C. B. (1964), Inorganic Ion Exchangers, Elsevier, Amsterdam, pp. 42–56.

    Google Scholar 

  43. Sideman, S., Hoffer, E., Better, O. S., and Lupovich, S. (1980), in Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 81–90.

    Google Scholar 

  44. Denti, E., Walker, J. M., Brancaccio, D., and Tessore, V. (1977), Med. Instr 11, 212.

    CAS  Google Scholar 

  45. Gurland, H. J., Castro, L. A., Samtleben, W., and Fernandez, J. C. (1978), Proc. Conf. Nondialytic Management Uremia, Brooklyn, NY.

    Google Scholar 

  46. Sideman, S., and Manor, D. (1982), Nephron 31, 1.

    Article  PubMed  CAS  Google Scholar 

  47. Alfrey, A. C., Le Gendre, G. R., and Kaehny, W. D. (1976), N. Engl. J. Med 294, 184.

    Article  PubMed  CAS  Google Scholar 

  48. Lewin, A. J., Greenbaum, M. A., Gordon, A., and Maxwell, M. H. (1972), Proc. Dialysis Transplant. Forum,p. 52.

    Google Scholar 

  49. Harrison, J. W. E., Trabin, B., and Martin, E. W. (1951), J. Pharmacol. Exp. Therapy 102, 179.

    Google Scholar 

  50. Clinton, L. T., and Shubert, J. (1948), J. Pharmacol. Exp. Therapy 94, 1.

    Google Scholar 

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Authors and Affiliations

  1. Departments of Chemical and Biomedical Engineering and Faculty of Medicine, and the Julius Silver Institute of Biomedical Engineering, Technion, Israel Institute of Technology, Haifa, 32000, Israel

    S. Sideman, N. Lotan, A. Tabak, D. Manor, L. Mor, U. Taitelman, J. Brook & A. Tzipiniuk

Authors
  1. S. Sideman
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  2. N. Lotan
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  3. A. Tabak
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  4. D. Manor
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  5. L. Mor
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  6. U. Taitelman
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  7. J. Brook
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  8. A. Tzipiniuk
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Editors and Affiliations

  1. Artificial Cells and Organ Research Center, McGill University, Montreal, Quebec, Canada

    T. M. S. Chang MD, PhD, FRCP (C) (Professor of Physiology and of Medicine, and Director) (Professor of Physiology and of Medicine, and Director)

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Sideman, S. et al. (1984). Tailor-Made Agarose-Based Reactive Beads for Hemoperfusion and Plasma Perfusion. In: Chang, T.M.S. (eds) Microencapsulation and Artificial Cells. Humana Press. https://doi.org/10.1007/978-1-4612-5182-8_19

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  • DOI: https://doi.org/10.1007/978-1-4612-5182-8_19

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9601-0

  • Online ISBN: 978-1-4612-5182-8

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