Abstract
Differentiation of cells is connected to changes in chromatin structure. Skeletal muscle cells were either labeled with 3H-thymidine, to estimate DNA synthesis and NAD+ was used as the precursor for poly(ADP-ribose)-synthesis in permeabilized cells. Parallel the fusion of myoblasts to myotubes were observed, different times of the cultivation period. At the time when myoblast fusion was high, DNA-synthesis was low, but poly(ADP-ribose)-synthesis was increasing. Autoradiographic data confirmed the results on semiconservative DNA-synthesis. Methoxybenzamide at very low concentrations stimulates poly(ADP-ribose)-synthesis and also myoblast fusion, at higher concentrations both processes are inhibited. Pulse chase experiments using a 5 min 3H-thymidine pulse showed that most of the specific radioactivity is located first in the micrococcus nuclease sensitive region, possibly as Okazaki units, which are elongated during the chase period and were found at that time also in the micrococcus nuclease resistant region. There are only small differences in spacer/core relationship at different times of myogenesis. During the most active fusion period newly synthesized poly(ADP-ribose) is located more in the MNase sensitive part of chromatin. At that time more DNA strandbreaks could be detected by nucleoidsedimentation technique.
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Török, O., Altmann, H. (1984). Chromatin Changes in Skeletal Muscle Cells During the Fusion to Myotubes. In: Skehan, P., Friedman, S.J. (eds) Growth, Cancer, and the Cell Cycle. Experimental Biology and Medicine, vol 5. Humana Press. https://doi.org/10.1007/978-1-4612-5178-1_2
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DOI: https://doi.org/10.1007/978-1-4612-5178-1_2
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