Isolation and Characterization of a Growth Regulatory Factor from 3T3 Cells
The mouse fibroblast line 3T3 (1) exhibits a form of growth control in vitro in that it reaches only a very low saturation density and can remain for long periods of time in a viable but nondividing state. This phenomenon, termed density-dependent inhibition of growth (2), was first described and most extensively studied in the 3T3 cell line. In this paper we review some recent evidence indicating that endogenous growth inhibitors may account for at least part of the growth inhibition observed in 373 fibroblast cultures and that such inhibitors may be isolated and characterized in terms of their functional interactions with target cells. The results of these studies suggest several useful refinements in our analysis of the mechanisms of growth control in cultured cells.
KeywordsFiltration Urea Electrophoresis Fractionation Polypeptide
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