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Isolation and Characterization of a Growth Regulatory Factor from 3T3 Cells

  • John L. Wang
  • Yen-Ming Hsu
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 5)

Abstract

The mouse fibroblast line 3T3 (1) exhibits a form of growth control in vitro in that it reaches only a very low saturation density and can remain for long periods of time in a viable but nondividing state. This phenomenon, termed density-dependent inhibition of growth (2), was first described and most extensively studied in the 3T3 cell line. In this paper we review some recent evidence indicating that endogenous growth inhibitors may account for at least part of the growth inhibition observed in 373 fibroblast cultures and that such inhibitors may be isolated and characterized in terms of their functional interactions with target cells. The results of these studies suggest several useful refinements in our analysis of the mechanisms of growth control in cultured cells.

Keywords

Growth Inhibitory Activity Mouse Embryo Fibroblast Growth Regulatory Factor Hormonally Define Medium High Molecular Weight Precursor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Todaro, G.J. and H. Green. 1963. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17:2. 99–313.CrossRefGoogle Scholar
  2. 2.
    Stoker, M.G.P. and H. Rubin. 1967. Density-dependent inhibition of cell growth in culture. Nature (Lond.) 215: 171–172.CrossRefGoogle Scholar
  3. 3.
    Steck, P.A., P.G. Voss, and J.L. Wang. 1979. Growth control in cultured 3T3 fibroblasts. Assays of cell proliferation and demonstration of a growth inhibitory activity. J. Cell Biol. 83: 562–575.PubMedCrossRefGoogle Scholar
  4. 4.
    Steck, P.A., J. Blenis, P.G. Voss, and J.L. Wang. 1982. Growth control in cultured 3T3 fibroblasts. II. Molecular properties of a fraction enriched in growth inhibitory activity. J. Cell Biol. 92: 523–530.PubMedCrossRefGoogle Scholar
  5. 5.
    Wang, J.L., P.A. Steck, and J.W. Kurtz. 1982. Growth control in cultured 3T3 fibroblasts. Molecular properties of a growth regulatory factor isolated from conditioned medium. In Growth of Cells in Hormonally Defined Media. D.A. Sirbasku, G.H. Sato, and A.B. Pardee, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. pp. 305–317.Google Scholar
  6. 6.
    Wells, V. and L. Mallucci. 1983. Properties of a cell growth inhibitor produced by mouse embryo fibroblasts. J. Cell. Physiol. 117: 148–154.PubMedCrossRefGoogle Scholar
  7. 6.
    Wells, V. and L. Mallucci. 1983. Properties of a cell growth inhibitor produced by mouse embryo fibroblasts. J. Cell. Physiol. 117: 148–154.PubMedCrossRefGoogle Scholar
  8. 8.
    Voss, P.G., P.A. Steck, J.C. Calamia, and J.L. Wang. 1982. Growth control in cultured 3T3 fibroblasts. III. Binding interactions of a growth inhibitory activity with target cells. Exp. Cell Res. 138: 397–407.CrossRefGoogle Scholar
  9. 9.
    Dulbecco, R. and M.G.P. Stoker. 1970. Conditions determining initiation of DNA synthesis in 3T3 cells. Proc. Natl. Acad. Sci. U.S.A. 66: 204–210.PubMedCrossRefGoogle Scholar
  10. 10.
    Whittenberger, B., D. Raben, M.A. Lieberman, and L. Glaser. 1978. Inhibition of growth of 3T3 cells by extract of surface membranes. Proc. Natl. Acad. Sci. U.S.A. 75: 5457–5461.PubMedCrossRefGoogle Scholar
  11. 11.
    Peterson, S.W., V. Lerch, M.E. Moynaham, M.P. Carson, and R. Vale. 1982. Partial characterization of a growth-inhibitory protein in 3T3 cell plasma membranes. Exp. Cell Res. 142: 447–451.CrossRefGoogle Scholar
  12. 12.
    Natraj, C.V. and P. Datta. 1978. Control of DNA synthesis in growing Balb/c 3T3 mouse cells by a fibroblast growth regulatory factor. Proc. Natl. Acad. Sci. U.S.A. 75: 6115–6119.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1984

Authors and Affiliations

  • John L. Wang
    • 1
  • Yen-Ming Hsu
    • 1
  1. 1.Department of BiochemistryMichigan State UniversityEast LansingUSA

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