Abstract
Lymphocytes treated with Concanavalin A (ConA) undergo a series of biochemical events leading to differentiation and division (reviewed by 1). Although the tumor promoter 12–0-tetradecanoylphorbol-l3-acetate, TPA, itself is not mitogenic, it can enhance the response of bovine lymphocytes to lectins by acting as an analogue of interleukin 1 (2). Thus, in the presence of a suboptimal dose of ConA or a limiting number of macrophages, TPA allows full proliferation. However, exposing the lymphocytes to TPA for about 24 hrs before stimulation with ConA greatly depresses the response (3). Furthermore, TPA blocks the mixed lymphocyte response (4).
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Iyer, A.P., Pishak, S.A., Sniezek, M.J., Mastro, A.M. (1984). Protein Kinase Activity in Lymphocytes; Effects of Concanavalin A and a Phorbol Ester. In: Skehan, P., Friedman, S.J. (eds) Growth, Cancer, and the Cell Cycle. Experimental Biology and Medicine, vol 5. Humana Press. https://doi.org/10.1007/978-1-4612-5178-1_14
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DOI: https://doi.org/10.1007/978-1-4612-5178-1_14
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