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Abstract

The mycobacteria are members of the order Actinomycetales and family Mycobacteriaceae. Species of the genus Mycobacterium show some evidence of branching, are acid-fast, are resistant to treatment with alkali, contain large amounts of lipids in their cell walls, and are aerobic or microaerophilic. Their growth rate is usually slow (2–6 weeks); however, a few species require only ≤5 days for growth. Most are resistant to conventional antibacterial and antituberculous antibiotics; however, some exhibit susceptibility to one or the other. Some are nonchromogenic, others may be photochromogenic or scotochromogenic. Current taxonomic trends recommend that members be referred to by their species name rather than by groupings.

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References

  1. Barksdale, L., and Kim, K. Mycobacterium. Bacteriol. Rev.41: 217, 1977.

    PubMed  CAS  Google Scholar 

  2. Frazier, A. R., Rosenow, E. C., Ill, and Roberts, G. D. Nocardiosis: a review of twenty-five cases occurring during 24 months. Mayo Clinic Proc. 50: 657, 1975.

    CAS  Google Scholar 

  3. Harrington, R., and Karlson, A. G. Differentiation between M. tuberculosisand M. bovisby in vitro procedures. Am. J. Vet. Res.27: 1193, 1967.

    Google Scholar 

  4. Kilburn, J. O., O’Donnell, K. F., Silcox, V. A., and David, H. L. Preparation of a stable mycobacterial Tween hydrolysis test substrate. Appl. Microbiol.26: 826, 1973.

    PubMed  CAS  Google Scholar 

  5. Kilburn, J. O., Silcox, V. A., and Kubica, G. P. Differential identification of mycobacteria. V. The tellurite reduction test. Am. Rev. Resp. Dis.99: 94, 1969.

    PubMed  CAS  Google Scholar 

  6. Konno, K. New chemical method to differentiate human-type tubercule bacilli from other mycobacteria. Science124: 985, 1956.

    Article  PubMed  CAS  Google Scholar 

  7. Kubica, G. P. Differential identification of mycobacteria. VII. Key features for identification of clinically significant mycobacteria. Am. Rev. Resp. Dis.107: 9, 1973.

    Google Scholar 

  8. Kubica, G. P., Jones, W. D., Jr., Abbott, V. D., Beam, R. E., Kilburn, J. O., and Cater, J. C., Jr. Differential identification of mycobacteria. I. Tests on catalase activity. Am. Rev. Resp. Dis.94: 400, 1966.

    PubMed  CAS  Google Scholar 

  9. Kubica, G. P., and Pool, G. L. Studies on the catalase activity of acid-fast bacilli. I. An attempt to subgroup these organisms on the basis of their catalase activities at different temperatures and pH. Am. Rev. Resp. Dis.81: 387, 1960.

    PubMed  CAS  Google Scholar 

  10. Morgan, M. A., Horstmeier, C. D., DeYoung, D. R., and Roberts, G. D. Comparison of a radiometric method (BACTEC) and conventional media for recovery of mycobacteria from smear negative specimens. J. Clin. Microbiol.18: 384, 1983.

    PubMed  CAS  Google Scholar 

  11. Murphy, D. B., and Hawkins, J. E. Use of urease disks in the identification of mycobacteria. J. Clin. Microbiol.1: 465, 1975.

    PubMed  CAS  Google Scholar 

  12. Roberts, G. D., Goodman, N. L., Heifets, L., Larsh, H. W., Lindner, T. H., McClatchy, J. K., McGinnis, M. R., Siddiqi, S. H., and Wright, P. Evaluation of BACTEC radiometric method for recovery of Mycobacteria and drug susceptibility testing of Mycobacterium tuberculosisfrom acid-fast positive specimens. J Clin. Microbiol.18: 689, 1983.

    PubMed  CAS  Google Scholar 

  13. Runyon, E. H., Selin, M. J., and Harris, H. W. Distinguishing mycobacteria by the niacin test. Am. Rev. Tuberc.79: 663, 1959.

    PubMed  CAS  Google Scholar 

  14. Steadham, J. E. Reliable urease test for identification of mycobacteria. J. Clin. Microbiol.10: 134, 1979.

    PubMed  CAS  Google Scholar 

  15. Tisdall, P. A., Roberts, G. D., and Anhalt, J. P. Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone. J. Clin. Microbiol. 10: 506, 1979.

    PubMed  CAS  Google Scholar 

  16. Tisdall, P. A., DeYoung, D. R., Roberts, G. D., and Anhalt, J. P. Identification of clinical isolates of Mycobacteria with gas-liquid chromatography: a 10-month follow-up study. J. Clin. Microbiol.16: 400, 1982.

    PubMed  CAS  Google Scholar 

  17. Toda, T. Y., Hagihara, Y., and Takeya, K. A simple urease test for the classifica¬tion of mycobacteria. Am. Rev. Resp. Dis. 83: 757, 1960.

    Google Scholar 

  18. Vestal, A. L. Procedures for the isolation and identification of mycobacteria. U.S. Department of Health, Education and Welfare, Public Health Service Publication (CDC) 75 – 8230, 1975.

    Google Scholar 

  19. Vestal, A. L., and Kubica, G. P. Differential identification of mycobacteria. III. Use of thiacetazone, thiophene-2-carboxylic acid hydrazide and triphe- nyltetrazolium chloride. Scand. J. Resp. Dis. 48: 142, 1967.

    CAS  Google Scholar 

  20. Virtanen, S. A study of nitrate reduction by mycobacteria. Acta Tuberc. Scand. (Suppl.) 48: 119, 1960.

    Google Scholar 

  21. Wayne, L. G. Recognition of Mycobacterium fortuitum by means of a three day phenolphthalein sulfatase test. Am. J. Clin. Pathol.36: 185, 1961.

    Google Scholar 

  22. Wayne, L. G., and Doubek, J. R. The role of air in the photochromogenic behavior of Mycobacterium kansasii. Am. J. Clin. Pathol.42: 431, 1964.

    Google Scholar 

  23. Wayne, L. G., and Doubek, J. R. Classification and identification of mycobacte¬ria. II. Tests employing nitrate and nitrite as substrates. Am. Rev. Resp. Dis.91: 738, 1965.

    PubMed  CAS  Google Scholar 

  24. Wayne, L. G., Doubek, J. R., and Russell, R. L. Classification and identification of mycobacteria. I. Tests employing Tween-80 as a substrate. Am. Rev. Resp. Dis.90: 588, 1964.

    PubMed  CAS  Google Scholar 

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© 1985 Mayo Foundation

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Roberts, G.D. (1985). Mycobacteria and Nocardia. In: Washington, J.A. (eds) Laboratory Procedures in Clinical Microbiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5070-8_6

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  • DOI: https://doi.org/10.1007/978-1-4612-5070-8_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9550-1

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