Abstract
A number of rat tissues, particularly those of the cardiovascular system (Guffroy & Strolin Benedetti, 1984 a; Lyles, 1984), contain an amine oxidase with a different substrate and inhibitor specificity from the flavin-dependent mitochondrial monoamine oxidase (MAO-A and B). The enzyme is resistant to MAO inhibiting concentrations (e.g. 1mM) of the acetylenic drugs, clorgyline and deprenyl, but is sensitive to carbonyl reagents such as semicarbazide which at similar concentrations produce little or no inhibition of MAO. Consequently, pyridoxal phosphate has been proposed as a possible cofactor for the semicarbazide-sensitive amine oxidase (SSAO). SSAO activity may conveniently be measured with low micromolar concentrations of the exogenous amine, benzylamine (Km around 5 µM).
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Guffroy, C., Boucher, T., Benedetti, M.S. (1985). Further Investigation of the Metabolism of Two Trace Amines, β-Phenylethylamine and p-Tyramine by Rat Aorta Semicarbazide-Sensitive Amine Oxidase. In: Boulton, A.A., Maitre, L., Bieck, P.R., Riederer, P. (eds) Neuropsychopharmacology of the Trace Amines. Humana Press. https://doi.org/10.1007/978-1-4612-5010-4_4
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DOI: https://doi.org/10.1007/978-1-4612-5010-4_4
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