Skip to main content
SpringerLink
Log in
Book cover

Vitamins and Cancer pp 35–49Cite as

Biochemical Mechanism of Inhibition of Phorbol Ester-Induced Mouse Epidermal Ornithine Decarboxylase by Retinoic Acid

  • Chapter

  • 88 Accesses

Part of the book series: Experimental Biology and Medicine, Vol. 10 ((EBAM,volume 10))

Abstract

Retinoic acid (vitamin A acid), a natural metabolite of retinal or retinyl esters, is required to maintain normal growth and differentiation of epithelial tissues (1–4). Since neoplastic transformation usually results in the loss of cellular differentiation, and vitamin A and its analogs (retinoids) play a role in maintaining the normal differentiation of epithelial cells, it is evident that retinoids may play a potential role in the development of cancer. Excellent reviews are available about the role of retinoids in the prevention and treatment of cancer (5–15). The exact molecular mechanism of action of retinoids remains undefined (16–22).

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Duca, H.F. Retinoic acid metabolism. Fed. Proc. 38:2519–2523, 1979.

    Google Scholar 

  2. Dowling, J.E., and Wald, G. The biological function of vitamin A acid. Proc. Natl. Acad. Sci. USA 46:587–608, 1960.

    Article  PubMed  CAS  Google Scholar 

  3. Wolbach, S.B., and Howe, P.R. Tissue changes following deprivation of fat soluble A vitamin. J. Exp. Med. 42:753–777, 1925.

    Article  PubMed  CAS  Google Scholar 

  4. Duca, H.F. The metabolism of vitamin A and its functions. In: Nutritional Factors in the Induction and Maintenance of Malignancy (eds. E.C. Butterworth, Jr. and M.L. Hutchinson), pp. 149–167, Academic Press, Inc., New York, 1983.

    Google Scholar 

  5. Mettlin, C., Graham, S., and Swanson, M. Vitamin A and lung cancer. J. Natl. Cancer Inst. 62:1435–1438, 1979.

    PubMed  CAS  Google Scholar 

  6. Hinds, M.W., Kolonel, L.N., Hankin, J.H., and Lee, J. Dietary vitamin A, carotene, vitamin C and risk of lung cancer in Hawaii. Am. J. Epidemiol. 119:227–237, 1984.

    PubMed  CAS  Google Scholar 

  7. Moore, T. Effects of vitamin A deficiency in animals: pharmacology and toxicology of vitamin A. In: The Vitamins (ed. Sebrell and Harris), 2nd edition, no. 1, pp. 245–266 and 280–294, Academic Press, New York, 1967.

    Google Scholar 

  8. Sporn, M.B., and Newton, D.L. Chemoprevention of cancer with retinoids. Fed. Proc. 38:2528–2534, 1979.

    PubMed  CAS  Google Scholar 

  9. Pawson, B.A., Ehmann, C.W., Itri, L.M., and Sherman, M.I. Retinoids at the threshold: Their biological significance and therapeutic potential. J. Medicinal Chem. 25: 1270–1277, 1982.

    Article  Google Scholar 

  10. Mayer, H., Bollag, W., Hänni, R., and Rüegg, R. Retinoids, a new class of compounds with prophylactic and therapeutic activities in oncology and dermatology. Experlentia 34:1105–1119, 1978.

    Article  CAS  Google Scholar 

  11. Moon, R.C., Mormick, D.L., and Mehta, R.G. Inhibition of carcinogenesis by retinoids. Cancer Res. (suppl.) 43:2469s-2475s, 1983.

    PubMed  CAS  Google Scholar 

  12. Sporn, M.B., and Roberts, A.B. Role of retinoids in differentiation and carcinogenesis. Cancer Res. 43:3034–3040, 1983.

    PubMed  CAS  Google Scholar 

  13. Bollag, W. Therapy of epithelial tumors with an aromatic retinoic acid analog. Chemotherapy 21;236–247, 1975.

    Article  PubMed  CAS  Google Scholar 

  14. Hill, D.L., and Grubbs, C.J. Retinoids as chemopreventive and anticancer agents in intact animals (review). Anticancer Res. 2:111–124, 1982.

    PubMed  CAS  Google Scholar 

  15. Elias, P.M., and William, M.L.: Retinoids, cancer and the skin. Arch. Dermatol. 117:160–180, 1981.

    Article  PubMed  CAS  Google Scholar 

  16. Yuspa, S.H. Retinoids and tumor promotion. In: Diet, Nutrition and Cancer (ed. Daphne A. Roe), pp. 95–109, Alan R. Liss, Inc., New York, 1983.

    Google Scholar 

  17. Kummet, T., and Meyskens, F.L. Vitamin A: A potential inhibitor of human cancer. Seminars in Oncology.10:281–289, 1983.

    PubMed  CAS  Google Scholar 

  18. Ong, D.E. A novel retinol-binding protein from rat, purification and partial characterization. J. Biol. Chem. 259: 1476–1482, 1984.

    PubMed  CAS  Google Scholar 

  19. Jetten, A.M. Modulation of cell growth by retinoids and their possible mechanism of action. Fed. Proc. 43:134–139, 1984.

    PubMed  CAS  Google Scholar 

  20. Sani, B.P., Dawson, M.I., Hobbs, P.D., Chan, R.L., and Schiff, L.J. Relationship between binding affinities to cellular retinoic acid binding protein and biological potency of a new series of retinoids. Cancer Res. 44:190–195, 1984.

    PubMed  CAS  Google Scholar 

  21. Goldstein, S.M., Moskowitz, M.A., and Levine, L. Inhibition of stimulated prostaglandin biosynthesis by retinoic acid in smooth muscle cells. Cancer Res. 44:120–125, 1984.

    PubMed  CAS  Google Scholar 

  22. Verma, A.K. Biochemical mechanism of modulation of skin carcinogenesis by retinoids: In: Retinoids (eds., Orfanos, Braun-Falco, Farber, Grupper, Polano, Schuppli), pp. 117–131, Springer-Verlag, New York, 1981.

    Google Scholar 

  23. Verma, A.K., Conrad, E.A., and Boutwell, R.K. Differential effects of retinoic acid and 7,8-benzo-flavone on the induction of mouse skin tumors by the complete carcinogenesis process and by the initiation-promotion regimen. Cancer Res. 42:3519–3525, 1982.

    PubMed  CAS  Google Scholar 

  24. Verma, A.K., Shapas, B.G., Rice, H.M., and Boutwell, R.K. Correlation of the inhibition by retinoids of tumor promoter-induced mouse epidermal ornithine decarboxylase activity and of skin tumor promotion. Cancer Res. 39:419–425, 1979.

    PubMed  CAS  Google Scholar 

  25. Verma, A.K., and Boutwell, R.K. Inhibition of tumor promoter-induced mouse epidermal ornithine decarboxylase activity and prevention of skin carcinogenesis by vitamin A analogs (retinoids). In: Polyamines in Biomedical Research (ed. A. Gaugas), pp. 185–202, John Wiley & Sons, Ltd., England, 1980.

    Google Scholar 

  26. Verma, A.K., Rice, H.M., Shapas, B.G., and Boutwell, R.K. Inhibition of 12-0-tetradecanoyl-phorbol-13-acetate-induced ornithine decarboxylase activity in mouse epidermis by vitamin A analogs (retinoids). Cancer Res. 38:793–801, 1978.

    PubMed  CAS  Google Scholar 

  27. Verma, A.K. Mouse skin carcinogenesis. In: Models in Dermatology (eds., Maibach, Lowe), vol. 2, Dermatopharmacology and Toxicology, pp. 313–321, Karger, Basel, 1985.

    Google Scholar 

  28. Boutwell, R.K. The function and mechanism of promoters of carcinogenesis. CRC Critical Rev. Toxicol. 2:419–443, 1974.

    Article  CAS  Google Scholar 

  29. Verma, A.K., and Boutwell, R.K. Effects of dose and duration of treatment with the tumor-promoting agent, 12-0-tetradecanoylphorbol-13-acetate on mouse skin carcinogenesis. Carcinogenesis l:271–276, 1980.

    Article  Google Scholar 

  30. Murray, A.W., and Fitzgerald, D.J. Tumor promoters inhibit metabolic cooperation in coculture of epidermal and 3T3 cells. Biochem. and Biophys. Res. Commun. 91:395–401, 1979.

    Article  CAS  Google Scholar 

  31. Blumberg, P.M. In vitro studies on the mode of action of the phorbol esters, potent tumor promoters: Part I. CRC Crit. Rev. Toxicol. 8, 153–197, 1980.

    Article  CAS  Google Scholar 

  32. Boutwell, R.K., Verma, A.K., Ashendel, C.L., and Astrup, E. Mouse skin: A useful model system for studying the mechanism of chemical carcinogenesis. In: Carcinogenesis, A Comprehensive Survey (eds.Hecker, Fusenig, Kunz, Marks and Thielmann), vol. 7, pp. 1–12, Raven Press, New York, 1982.

    Google Scholar 

  33. Weinstein, I.B., Gattoni-Celli, S., Kirschmeier, P., Hsiao, W., Horowitz, A., and Jeffrey, A. Cellular targets and host genes in multi-stage carcinogenesis. Fed. Proc. 43:2287–2294, 1984.

    PubMed  CAS  Google Scholar 

  34. O’Brien, T.G. The induction of ornithine decarboxylase as an early, possibly obligatory, event in mouse skin carcinogenesis. Cancer Res. 36:2644–2653, 1976.

    PubMed  Google Scholar 

  35. Takigawa, M., Verma, A.K., Simsiman, R.C., and Boutwell, R.K. Inhibition of mouse skin tumor promotion and of promoter-stimulated epidermal polyamine biosynthesis by ordifluoromethylornithine. Cancer Res. 43: 3732–3738, 1983.

    PubMed  CAS  Google Scholar 

  36. Weeks, C.E., Herrmann, A.L., Nelson, F.R., and Slaga, T.J. oifluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, inhibits tumor promoter-induced polyamine accumulation and carcinogenesis in mouse skin. Proc. Natl. Acad. Sci. USA 79:6028–6032, 1982.

    Article  PubMed  CAS  Google Scholar 

  37. Jänne, J., Pösö, H., and Raina, A. Polyamines in rapid growth and cancer. Biochim. Biophys. Acta. 473:241–293, 1978.

    PubMed  Google Scholar 

  38. Raina, A., and Jänne, J. Polyamines as cellular regulators. Medical Biology 59:269–461, 1981.

    Google Scholar 

  39. Seely, J.E., and Pegg, A.E. Changes in mouse kidney ornithine decarboxylase activity are brought about by changes in the amount of enzyme protein as measured by radioimmunoassay. J. Biol. Chem. 258:2496–2500, 1983.

    PubMed  CAS  Google Scholar 

  40. Metcalf, B.W., Bey, P., Danzin, D., Jung, M.J., Casara, P., and Vevert, J.P. Catalytic irreversible inhibition of mammalian ornithine decarboxylase (E.C. 4.1.1.17) by substrate and product analogues. J. Am. Chem. Soc. 100:2551–2553, 1978.

    Article  CAS  Google Scholar 

  41. Verma, A.K., and Boutwell, R.K. Vitamin A acid (retinoic acid), a potent inhibitor of 12-0-tetra-decanoyl-phorbol-13-acetate-induced ornithine decarboxylase activity in mouse epidermis. Cancer Res 37: 2196–2201, 1977.

    PubMed  CAS  Google Scholar 

  42. Pritchard, M.L., Seeley, J.E., Pösö, H., Jefferson, L.S., and Pegg, A.E. Binding of radioactive a-difluoromethylornithine to rat liver ornithine decarboxylase. Biochem. Biophys. Res. Commun. 100:1597–1603, 1981.

    Article  PubMed  CAS  Google Scholar 

  43. Matsufuji, S., Fujita, K., Kameji, T., Kanamoto, R., Murakami, Y., and Hayashi, Shinichi. A monoclonal antibody to rat liver ornithine decarboxylase. J. Biochem. 96:1525–1530, 1984.

    PubMed  CAS  Google Scholar 

  44. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227:680–685, 1970.

    Article  CAS  Google Scholar 

  45. Towbin, H., Staehelin, T., and Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and applications. Proc. Natl. Acad. Sci. USA 76:4350–4354, 1979.

    Article  PubMed  CAS  Google Scholar 

  46. Glass, W.F., Briggs, R.C., and Hnilica, L.S. Identification of tissue-specific nuclear antigens transferred to nitrocellulose from polyacrylamide gels. Science 211:70–72, 1981.

    Article  PubMed  CAS  Google Scholar 

  47. Bio-Rad Immuno-Blot Assay Kit Instruction Manual. GAR-HRP (Goat Anti-Rabbit I) or GAM-HRP (Goat Anti-Mouse I).

    Google Scholar 

  48. O’Brien, T.G., Madara, T., and Ponsell, K. Changes in ornithine decarboxylase (ODC) activity caused by tumor promoters are due to changes in the amount of enzyme protein. Proc. Am. Assoc. Cancer Res. 25:77, 1984.

    Google Scholar 

  49. Ross, J. Precursor of globin messenger RNA. J. Mol. Biol. 106:403–420, 1976.

    Article  PubMed  CAS  Google Scholar 

  50. Thomas, P.S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77:5201–5205, 1980.

    Article  PubMed  CAS  Google Scholar 

  51. Wahl, G.M., Stern, M., and Stark, G.R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc. Natl. Acad. Sci. USA 76:3683–3687, 1979.

    Article  PubMed  CAS  Google Scholar 

  52. Monlogue, L., Gupta, M., Wu, L., and Coffino, P. Molecular cloning and expression of the mouse ornithine decarboxylase gene. Proc. Natl. Acad. Sci. USA 81: 540–544, 1984.

    Article  Google Scholar 

  53. Ashendel, C.L., and Boutwell, R.K. Direct measurement of specific binding of highly lipophilic phorbol diester to mouse epidermal membranes using cold acetone. Biochem. Biophys. Res. Commun. 99:543–549, 1981.

    Article  PubMed  CAS  Google Scholar 

  54. Solanki, V., and Slaga, T.J. The down-modulation of receptors for phorbol ester tumor promoter in primary epidermal cells. Carcinogenesis 3:993–998, 1982.

    Article  PubMed  CAS  Google Scholar 

  55. Kikkawa, U., Takai, Y., Tanaka, Y., Miyake, R., and Nishizuka, Y. Protein kinase C as a possible receptor protein of tumor promoting phorbol esters. J. Biol. Chem. 258:11442–11445, 1983.

    PubMed  CAS  Google Scholar 

  56. Niedel, J.E., Kuhn, L.J., and Vandenbark, G.R. Phorbol diester receptor copurifies with protein kinase C. Proc. Natl. Acad. Sci. USA 80:36–40, 1983.

    Google Scholar 

  57. Ashendel, C.L., Staller, J.M., and Boutwell, R.K. Solubilization, purification, and reconstitution of a phorbol ester receptor from the particulate protein fraction of mouse brain. Cancer Res. 43:4327–4332, 1983.

    PubMed  CAS  Google Scholar 

  58. Verma, A.K., and Boutwell, R.K. Intracellular calcium and skin tumor promotion: calcium regulation of the induction of epidermal ornithine decarboxylase activity by the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate. Biochem. Biophys. Res. Commun. 101:375–383, 1981.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Oncology, Wisconsin Clinical Cancer Center, University of Wisconsin, Center for Health Sciences, 53792, Madison, WI, USA

    Ajit K. Verma

Authors
  1. Ajit K. Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Section of Hematology/Oncology, Cancer Center, Arizona Health Sciences Center, Tucson, AZ, USA

    Frank L. Meyskens Jr.

  2. Center of Vitamins and Cancer Research Department of Radiology, University of Colorado Health Sciences Center, Denver, CO, USA

    Kedar N. Prasad

Rights and permissions

Reprints and permissions

Copyright information

© 1986 The Humana Press Inc.

About this chapter

Cite this chapter

Verma, A.K. (1986). Biochemical Mechanism of Inhibition of Phorbol Ester-Induced Mouse Epidermal Ornithine Decarboxylase by Retinoic Acid. In: Meyskens, F.L., Prasad, K.N. (eds) Vitamins and Cancer. Experimental Biology and Medicine, Vol. 10, vol 10. Humana Press. https://doi.org/10.1007/978-1-4612-5006-7_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-5006-7_3

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9395-8

  • Online ISBN: 978-1-4612-5006-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation