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Cloned HLA-D Genes: Characterisation and Approaches to Expression and Analysis of Function

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Human T Cell Clones

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 9))

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Abstract

The HLA-D region (Class II) genes determine cell surface glycoproteins which are heterodimers of an α and β chain, and which function in the immune response. This brief paper contains a summary of some of the current studies in this laboratory on the organisation of human Class II genes. Details on the characterisation of six different Class IIα chain genes are presented, including a new gene, DZα, which does not fit into any of the three most clearly established sets of loci: DP,DQ and DR(l). The genes for which we have most sequence information, and the most detailed map, are in the DP region(2). We have succeeded in transferring some of these cloned HLA-DP genes into mouse L cells and can detect expression of DP antigens on the cell surface. The transfectants are apparently capable of inducing a human T lymphocyte clone to proliferate specifically in response to presentation of the neuraminidase glycoprotein of influenza A virus along with DPw2 ClassII determinants(3).

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Author information

Authors and Affiliations

  1. Imperial Cancer Research Fund, Lincoln’s Inn Fields, London, UK

    John Trowsdale, Penelope Austin, Susan Carson, Adrian Kelly & John Young

  2. Tumour Immunology Unit, Department of Zoology, University College, London, UK

    Jonathan Lamb

Authors
  1. John Trowsdale
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  2. Penelope Austin
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  3. Susan Carson
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  4. Adrian Kelly
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  5. Jonathan Lamb
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  6. John Young
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Editor information

Editors and Affiliations

  1. Imperial Cancer Research Fund, Tumour Immunology Unit, Department of Zoology, University College London, London, UK

    Marc Feldmann

  2. MRC Tuberculosis and Related Infections, Hammersmith Hosptial, London, UK

    Jonathan R. Lamb

  3. Tissue Bank, Naval Medical Research Institute, Bethesda, Maryland, USA

    James N. Woody

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© 1985 The Humana Press Inc.

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Trowsdale, J., Austin, P., Carson, S., Kelly, A., Lamb, J., Young, J. (1985). Cloned HLA-D Genes: Characterisation and Approaches to Expression and Analysis of Function. In: Feldmann, M., Lamb, J.R., Woody, J.N. (eds) Human T Cell Clones. Experimental Biology and Medicine, vol 9. Humana Press. https://doi.org/10.1007/978-1-4612-4998-6_5

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  • DOI: https://doi.org/10.1007/978-1-4612-4998-6_5

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9391-0

  • Online ISBN: 978-1-4612-4998-6

  • eBook Packages: Springer Book Archive

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