Molecular Studies of the Human T-Cell Antigen Receptor

  • Mary K. L. Collins
  • A. Maija Kissonerghis
  • M. Jenny Dunne
  • Michael J. Owen
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 9)

Abstract

The T cell receptor for antigen has been identified in both mouse and man as a 90-kd disulphide-linked heterodimer comprised of one α chain (~ 50,000 mol.wt.) and one β chain (~ 40,000 mol.wt.) (1–3). Recently, cDNA clones encoding one of the chains of the T-cell antigen receptor have been isolated from mouse (4) and human (5) T-cell libraries. Sequence analysis of these cDNA clones demonstrates that they contain variable, joining and constant regions similar to those encoding immunoglobulins (5,6). Furthermore, the cDNA clones hybridise to gene sequences capable of undergoing somatic rearrangements in T-cells (4). The mouse and human cDNA probes most probably detect the β chain family of the T-cell antigen receptor since partial protein sequence analysis of a human T-cell β chain agrees with the translated nucleotide sequence of the human cDNA clone (7).

Keywords

Lymphoma Codon Recombination Polypeptide eDNA 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Allison, J.P., Mclntyre, R.W. and Black, D. J. Immunol. 129, 2293 (1982).PubMedGoogle Scholar
  2. 2.
    Haskins, K., Kubo, R., White, J., Pigeon, M., Kappler, J. and Marrack, P. J. Exp. Med. 157, 1149 (1983).PubMedCrossRefGoogle Scholar
  3. 3.
    Meuer, S.C., Fitzgerald, K.A., Hussey, R.E., Hodgdon, J.C., Schlossman, S.F. and Reinherz, E.L. J. Exp. Med. 155, 705 (1983).CrossRefGoogle Scholar
  4. 4.
    Hedrick, S.M., Cohen, D.I., Nielson, E.A. and Davis, M.M. Nature 308, 149 (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    Yanagi, Y., Yoshikai, Y., Leggett, K., Clark, S.P., Aleksander, I. and Mak, T. Nature 308, 145 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    Hedrick, S.M., Nielsen, E.A., Kavaler, J., Cohen, D.I. and Davis, M.M. Nature 308, 153 (1984).PubMedCrossRefGoogle Scholar
  7. 7.
    Acuto, O., Fabbi, M., Smart, J., Poole, C., Protentis, J., Roger, H., Schlossman, S. and Reinherz, E. Proc. Natl. Acad. Sci. USA. 86, 3851.Google Scholar
  8. 8.
    Caccia, N., Kronenberg, M., Saxe, D., Haars, R., Bruns, G.A.P., Goverman, J., Malissen, M., Willard, H., Yoshikai, Y., Simon, M., Hood, L. and Mak, T.W. Cell, 37, 1091 (1984).PubMedCrossRefGoogle Scholar
  9. 9.
    Croce, C.M., Shander, M., Martinis, J., Cicurel, L., D’Ancona, G.G., Dolby, T.W. and Koprowski, H. Proc. Natl. Acad. Sci. USA. 76, 3416 (1979).PubMedCrossRefGoogle Scholar
  10. 10.
    Malcolm, S., Barton, P., Murphy, C., Ferguson-Smith, M. A., Bentley, D.K. and Rabbits, T.H. Proc. Natl. Acad. Sci. USA 79, 4957 (1982).PubMedCrossRefGoogle Scholar
  11. 11.
    McBride, O.W., Heiter, P.A., Hollis, G.F., Swan, D., Otey, M.C. and Leder, P. J. Exp. Med. 157, 705 (1982).Google Scholar
  12. 12.
    Hengartner, H., Mes, T. and Muller, E. Proc. Natl. Acad. Sci. USA 75, 4494 (1977).CrossRefGoogle Scholar
  13. 13.
    Perry, R.P. Cell 33, 647 (1983).PubMedCrossRefGoogle Scholar
  14. 14.
    Gascoigne, N.R.J., Chien, Y., Becker, D.M., Kavaler, J. and Davis, M.M. Nature 310, 387 (1984).PubMedCrossRefGoogle Scholar
  15. 15.
    Yoshikai, Y., Antoniou, D., Clark, S.P., Yanagi, Y., Sangster, R., Nelson, D., Terhorst, C. and Mak, T.W. Nature (in press) (1984).Google Scholar
  16. 16.
    Siu, G., Clark, S.P., Yoshikai, Y., Malissen, M., Yanagi, Y., Strauss, E., Mak, T.W. and Hood, L. Cell 37, 393 (1984).PubMedCrossRefGoogle Scholar
  17. 17.
    Early, P., Huang, M., Davis, M., Calame, K. and Hood, L. Cell 19, 981 (1980).PubMedCrossRefGoogle Scholar
  18. 18.
    Yoshikai, Y., Yanagi, Y., Suciu-Foca, N. and Mak, T.W. Nature 310, 506 (1984).PubMedCrossRefGoogle Scholar
  19. 19.
    Clark, S.P., Yoshikai, Y., Taylor, S., Siu, G., Hood, L. and Mak, T.W. Nature 311, 387 (1984).PubMedCrossRefGoogle Scholar
  20. 20.
    Kavaler, J., Davis, M.M. and Chien, Y-L. Nature 310, 421 (1984).PubMedCrossRefGoogle Scholar
  21. 21.
    Toyonaga, B., Yanagi, Y., Suciu-Foca, N., Minchen, M. and Mak, T.W. Nature 311, 385 (1984).PubMedCrossRefGoogle Scholar
  22. 22.
    Siu, G., Kronenberg, M., Strauss, E., Maars, R., Mak, T.W. and Hood, L. Nature 311, 344 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1985

Authors and Affiliations

  • Mary K. L. Collins
    • 1
  • A. Maija Kissonerghis
    • 1
  • M. Jenny Dunne
    • 1
  • Michael J. Owen
    • 1
  1. 1.Imperial Cancer Research Fund Tumour Immunology Unit, Department of ZoologyUniversity College LondonLondonUK

Personalised recommendations