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Loss of Alloreactivity Associated with Acquired Suppressive and Natural Killer-Like Activities of Aged T Cell Clones

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Human T Cell Clones

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 9))

Abstract

The production of human T cell clones is now an established procedure in a number of laboratories. The relative ease with which antigen-specific or alloreactive lymphoblasts can be cloned and propagated in interleukin 2 (IL 2) makes this technique attractive for analysing the molecular and genetic bases of lymphocyte activation, regulation and effector functions of isolated populations. A widespread expectation amongst investigators in this area was that such cloned lines could be established as functionally stable, immortal sources of homogeneous reagents. However, few reports in the literature systematically address the question of the long-term functional stability, longevity, or percentage of immortal clones obtained in each particular experiment, and whether the generation of “permanent” lines is the exception rather than the rule. In several cases, the continuous culture of cloned human T cells with various reactivities for periods of many months or even years has been reported (1–11). Nonetheless, this is not necessarily a universal finding, particularly for T cell clones which respond proliferatively against alloantigens and which are thus of value as primed lymphocyte typing (PLT) reagents (12–19).

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Author information

Authors and Affiliations

  1. Immunology Laboratory, Medizinische Klinik, D-7400, Tübingen, Germany

    G. Pawelec & P. Wernet

Authors
  1. G. Pawelec
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  2. P. Wernet
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Editor information

Editors and Affiliations

  1. Imperial Cancer Research Fund, Tumour Immunology Unit, Department of Zoology, University College London, London, UK

    Marc Feldmann

  2. MRC Tuberculosis and Related Infections, Hammersmith Hosptial, London, UK

    Jonathan R. Lamb

  3. Tissue Bank, Naval Medical Research Institute, Bethesda, Maryland, USA

    James N. Woody

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© 1985 The Humana Press Inc.

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Pawelec, G., Wernet, P. (1985). Loss of Alloreactivity Associated with Acquired Suppressive and Natural Killer-Like Activities of Aged T Cell Clones. In: Feldmann, M., Lamb, J.R., Woody, J.N. (eds) Human T Cell Clones. Experimental Biology and Medicine, vol 9. Humana Press. https://doi.org/10.1007/978-1-4612-4998-6_30

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  • DOI: https://doi.org/10.1007/978-1-4612-4998-6_30

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9391-0

  • Online ISBN: 978-1-4612-4998-6

  • eBook Packages: Springer Book Archive

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