Abstract
We have identified cDNAs encoding the human interleu- kin-2 (IL-2) receptor from a cDNA library constructed from HUT-102B2 cell mRNA, and have expressed them in eukaryotic cells. Based on the deduced amino acid sequence from the DNA sequence, the IL-2 receptor is initially synthesized as a preprotein of 272 amino acids and then processed to a mature form of 251 amino acids. The protein has a very short positively charged cytoplasmic region at the carboxy end of the molecule that contains potential phosphorylation sites. The protein has two potential N-linked carbohydrate addition sites and multiple potential 0-linked carbohydrate addition sites. Although the IL-2 receptor appears to be encoded by a single structural gene, there are two distinct mRNAs that encode the protein that differ in the polyaden- ylation signal used. Further, there is evidence that alternate mRNA splicing may also occur.
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References
Morgan, D.A., Ruscetti, F.W. & Gallo, R.C. Science 193, 1007–1008 (1976).
Watson, J. J. exp. Med. 150, 1510–1519 (1979).
Coutinho, A., Larsson, E-L, Gronvik, K-O, & Andersson, J. Eur. J. Immun. 9, 587–592 (1979).
Gillis, S., Baker, P.E., Ruscetti, F.W. & Smith, K.A. J. exp. Med. 148, 1093–1098 (1978).
Robb, R.J., Kutny, R.M. & Chowdhry, V. Proc. natn. Acad. Sei. U.S.A. 80, 5990–5994 (1983)
Taniguchi, T., Matsui, H., Fujita, T., Takeoka, C., Kashima, N., Yoshimoto, R. & Hamura, J. Nature 302, 305–309 (1983).
Fujita, T., Takeoa, C., Matsui, H. & Taniguchi, T. Proc. natn. Acad. Sei. U.S.A. 81., 1634–1638 (1983).
Holbrook N.J., Smith, K.A., Fornace, A.J., Jr.. Comeau, C.M., Wiskocil, R.L., and Crabtree, G.R. Proc. natn. Acad. Sei. U.S.A. 81, 1634–1638 (1984).
Leonard, W.J., Depper, J.M., Uchiyama, T., Smith, K.A., Waldmann, T.A. & Greene, W.C. Nature 300, 267–269 (1982).
Leonard, W.J., Depper, J.M., Robb, R.J., Waldmann, T.A., & Greene, W.C. Proc. natn. Acad. Sei. 80, 6957–6961 (1983).
Wano, Y., Uchiyama, T., Fukui, K., Maeda, M., Uchino, H., and Yodoi, J. J. Immun. 132, 3005–3010 (1984).
Leonard, W.J., Depper, J.M., Waldmann, T.A., & Greene, W.C., in Receptors and Recognition, Series B, Vol 17, (ed., Greaves, M.). pp 45–66 (1984).
Leonard. W.J., Depper, J.M., Kro’nke, M., Robb, R.J., Waldmann, T.A. & Greene, W.C. J. Bio!. Chem. (in the press).
Depper, J.M., Leonard, W.J., Krönke, M., Waldmann, T.A. & Greene, W.C. J. Immun. 133, 1691–1695 (1984).
Leonard, W.ü., Depper, J.M., Crabtree, G.R., Rudikoff, S., Pumphrey, J., Robb, R.J., Kronke, M., Svetlik, P.B., Peffer, N.J., Waldmann, T.A. & Greene, W.C. Nature (in the press).
Uchiyama, T., Broder, S. & Waldmann, T.A. J. Immun. 126, 1393–1397 (1981)
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Leonard, W.J. et al. (1985). Molecular Cloning and Expression of cDNAS Encoding the Human Interleukin-2 Receptor. In: Feldmann, M., Mitchison, N.A. (eds) Immune Regulation. Experimental Biology and Medicine, vol 8. Humana Press. https://doi.org/10.1007/978-1-4612-4996-2_24
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DOI: https://doi.org/10.1007/978-1-4612-4996-2_24
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