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Adenovirus Entry into Cells: Some New Observations on an Old Problem

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Concepts in Viral Pathogenesis II

Abstract

With the advent of electron microscopy, it became possible to investigate the pathway of viral entry into cells. Viruses were visualized bound to the cell surface prior to entry and within cells after entry. Some investigators interpreted their findings to indicate that a virus such as adenovirus entered the cell in a vacuole or vesicle whose formation was induced by the presence of the virus at the cell surface. Other investigators concluded that viruses directly crossed the plasma membrane without being transported in a vesicle.

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References

  1. Pastan I, Willingham MC (1983) Receptor-mediated endocytosis: Coated pits, receptosomes, and the Golgi. Trends Biochem Sci 8:250–254.

    Article  CAS  Google Scholar 

  2. Pastan I, Willingham MC (1981) Journey to the center of the cell: Role of the receptosome. Science 214:504–509.

    Article  CAS  PubMed  Google Scholar 

  3. Helenius A, Mellman I, Wall D, Hubbard A (1983) Endosomes. Trends Biochem Sci 8:245–250.

    Article  CAS  Google Scholar 

  4. Goldstein JL, Anderson RGW, Brown MS (1979) Oated pits, coated vesicles, and receptor-mediated endocytosis. Nature 279:679–685.

    Article  CAS  PubMed  Google Scholar 

  5. Maxfield RF, Cheng S-y, Dragsten P, Willingham MC, Pastan I (1981) Binding and mobility of the cell surface receptors for 3,3’, 5-triiodo-L-thyronine. Science 211:63–65.

    Article  CAS  PubMed  Google Scholar 

  6. Willingham MC, Pastan I (1983) Formation of receptosomes from plasma mem-brane coated pits during endocytosis: Analysis by serial sections with improved membrane labeling and preservation techniques. Proc Natl Acad Sci USA 80:5617–5621.

    Article  CAS  PubMed  Google Scholar 

  7. Willingham MC, Pastan I (1984) Do coated vesicles exist? Trends Biochem Sci 9:93–94.

    Article  Google Scholar 

  8. Dales S (1973) Early events in cell-animal virus interactions. Bacteriol Rev 37:103–135.

    CAS  PubMed Central  PubMed  Google Scholar 

  9. FitzGerald DJP, Padmanabhan R, Pastan I, Willingham MC (1983) Adenovirus- induced release of epidermal growth factor and Pseudomonas toxin into the cy¬tosol of KB cells during receptor-mediated endocytosis. Cell 32:607–617.

    Article  CAS  PubMed  Google Scholar 

  10. Tycko B, Maxfield FR (1982) Rapid acidification of endocytic vesicles containing a2-macroglobulin. Cell 28:643–651.

    Article  CAS  PubMed  Google Scholar 

  11. Seth P, Willingham MC, Pastan I (1985) Binding of adenovirus and its external proteins to Triton X-114: Dependence on pH. J Biol Chem 260:14431–14434.

    CAS  PubMed  Google Scholar 

  12. Okada CY, Rechsteiner M (1982) Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. Cell 29:33–41.

    Article  CAS  PubMed  Google Scholar 

  13. Dickson RB, Beguinot L, Hanover JA, Richert ND, Willingham MC, Pastan I (1983) Isolation and characterization of a highly enriched preparation of recep-tosomes (endosomes) from a human cell line. Proc Natl Acad Sci USA 80:5335–5339.

    Article  CAS  PubMed  Google Scholar 

  14. Seth P, Willingham MC, Pastan I (1984) Adenovirus-dependent release of 51Cr from KB cells at an acidic pH. J Biol Chem 259:14350–14353.

    CAS  PubMed  Google Scholar 

  15. Marsh M, Helenius A (1980) Adsorptive endocytosis of Semliki Forest virus. J Mol Biol 142:439–454.

    Article  CAS  PubMed  Google Scholar 

  16. Seth P, FitzGerald DJP, Willingham MC, Pastan I (1984) Role of a low-pH en-vironment in adenovirus enhancement of the toxicity of a Pseudomonas exotoxin- epidermal growth factor conjugate. J Virol 51:650–655.

    CAS  PubMed Central  PubMed  Google Scholar 

  17. Seth P, Pastan I, Willingham MC (1985) Adenovirus-dependent increase in cell membrane permeability. J Biol Chem 261:9589–9602.

    Google Scholar 

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© 1986 Springer Science+Business Media New York

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Pastan, I., Seth, P., FitzGerald, D., Willingham, M. (1986). Adenovirus Entry into Cells: Some New Observations on an Old Problem. In: Notkins, A.L., Oldstone, M.B.A. (eds) Concepts in Viral Pathogenesis II. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4958-0_17

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  • DOI: https://doi.org/10.1007/978-1-4612-4958-0_17

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9375-0

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