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Inhibitor(s) of Biologically Active Erythropoietin in Concentrated Human Sera

  • J. Barone-Varelas
  • C. Morley
  • W. Fried
Conference paper
Part of the Experimental Hematology Today book series (HEMATOLOGY, volume 1985)

Abstract

The most specific assay for biologically active erythropoietin (Ep) is the exhypoxic, polycythemic mouse assay [1]. However, the sensitivity of this assay is still not sufficient to detect normal circulating levels of Ep; these have been established by radioimmunoassay to be 9.7 to 24.8 mUnits/ml of serum [2]. All previous efforts at concentrating human serum prior to assaying its biologically active Ep content have resulted in the loss of some activity [3]. We report here results of studies designed to identify a method of concentrating human serum without loss of its biologically active Ep content, and when unsuccessful in this endeavour, studies were initiated to determine why Ep activity is invariably lost after concentrating human sera.

Keywords

Normal Human Serum Concentration Procedure Filtration Cone Renal Tissue Homogenate Active Erythropoietin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • J. Barone-Varelas
    • 1
  • C. Morley
    • 1
  • W. Fried
    • 1
  1. 1.Departments of Biochemistry and MedicineRush Presbyterian St. Luke’s Medical CenterChicagoUSA

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