Inhibitor(s) of Biologically Active Erythropoietin in Concentrated Human Sera

  • J. Barone-Varelas
  • C. Morley
  • W. Fried
Conference paper
Part of the Experimental Hematology Today book series (HEMATOLOGY, volume 1985)


The most specific assay for biologically active erythropoietin (Ep) is the exhypoxic, polycythemic mouse assay [1]. However, the sensitivity of this assay is still not sufficient to detect normal circulating levels of Ep; these have been established by radioimmunoassay to be 9.7 to 24.8 mUnits/ml of serum [2]. All previous efforts at concentrating human serum prior to assaying its biologically active Ep content have resulted in the loss of some activity [3]. We report here results of studies designed to identify a method of concentrating human serum without loss of its biologically active Ep content, and when unsuccessful in this endeavour, studies were initiated to determine why Ep activity is invariably lost after concentrating human sera.


Normal Human Serum Concentration Procedure Filtration Cone Renal Tissue Homogenate Active Erythropoietin 
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  1. 1.
    Weintraub AH, Gordon AS, Camiscoli JF (1966) Use of the hypoxic induced polycythemic mouse in the assay and standardization of erythropoietin. J Lab Clin Med 67: 742Google Scholar
  2. 2.
    Garcia JF, Ebbe SN, Hollander L, Cutting HO, Miller ME,Cronkite EP (1982) Radioimmuno¬assay of erythropoietin: circulating levels in normal and polycythemic human beings.J Lab Clin Med 99: 624Google Scholar
  3. 3.
    Erslev AJ, Caro J, Kansu E, Miller 0, Cobbs E. Plasma erythropoietin in polycythemia. Am J Med 66: 243Google Scholar
  4. 4.
    Cohen RA, Clemons G, Ebbe S (1985) Correlation between bioasay and radioimmunoassay for erythropoietin in human serum and urine concentrates Proc Soc Exp Biol Med 179: 296, 1985Google Scholar
  5. 5.
    Krystal G, Eaves AC, Eaves CJ (1981) Determination of hormonal human serum erythropoietin levels, using mouse bone marrow. J Lab Clin Med 97: 158PubMedGoogle Scholar
  6. 6.
    Dunn CD, Lange RD (1978) Erythroid regulat¬ing factors: correction of detectable titers in vivo and in vitro. Blood 52: 1238PubMedGoogle Scholar
  7. 7.
    Radtke HW, Erbes PM, Fassbinder W, Koch KM (1978) Serum erythropoietin measurements using the fetal mouse liver cell culture: the importance of reduction in variation in the specific activity of radioiron transferrin Exp Hemat 6: 468PubMedGoogle Scholar
  8. 8.
    Moriyama Y, Saito H, Kinioshita Y (1970) Erythropoietin inhibitor in plasma from patients with chronic renal failure. Haematologica 4: 15Google Scholar
  9. 9.
    Moriyama Y, Shimotori T (1970) Studies on erythropoiesis inhibiting factor (EIF). II. the site of formation of EIF. Acta Haemat 44: 321Google Scholar
  10. 10.
    Reynafarge C (1968) Humoral regulation of the erythropoietic depression of high altutide polycythemic subjects after return to sea level. Ann N Y Acad Sci 149: 472CrossRefGoogle Scholar
  11. 11.
    Whitcombe WH, Moore M, Dille R, Hummer L, Bird RM (1965) Clin Invest 44: 1110Google Scholar
  12. 12.
    Erslev AJ, Thorling EB (1968) Effect of polycythemic serum on the rate of red cell production. Ann N Y Acad Sci 149: 173PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • J. Barone-Varelas
    • 1
  • C. Morley
    • 1
  • W. Fried
    • 1
  1. 1.Departments of Biochemistry and MedicineRush Presbyterian St. Luke’s Medical CenterChicagoUSA

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