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Properties of a T-Lymphocyte Derived Differentiation Inducing Factor (DIF) for the Myeloid Leukemic Cell Line HL-60

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Experimental Hematology Today—1985

Part of the book series: Experimental Hematology Today ((HEMATOLOGY,volume 1985))

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Abstract

Mitogen-stimulated mononuclear blood cells produce a differentiation inducing factor (DIF) for the promye1ocytic cell line HL-60. This factor is produced constitutively by the T-lymphocyte line HUT-102, which therefore can be used as a source for purification of DIF. High resolution ion exchange chromatography revealed considerable charge heterogeneity of DIF, which was confirmed by chromatofocusing. There is a marked synergistic inducing effect between DIF and retinoic acid. In addition we found additive differentiation effects between DIF and DMSO, Ara-C, Actinomycin D, PGE2 and choleratoxin. The effect did not depend on the normal rate of RNA synthesis, as it was not blocked by Cordycepin or α-aminitin. In addition to its maturation effects on HL-60 with the production of monocyte-1ike cells, we showed that DIF inhibited the CSF-stimulated production of neutrophils from normal immature myeloid bone marrow cells in liquid culture. This suggests that DIF takes part in the regulation of hemopoiesis. DIF has been shown to induce differentiation of some leukemic cell lines and to inhibit growth in clonal assays of subclones of myeloid cell lines as well as fresh leukemia cells from patients with acute myeloid leukemia. Therefore, DIF may be interesting from a clinical point of view.

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Authors and Affiliations

  1. Division of Hematology, Department of Medicine, University of Lund, 221 85, Lund, Sweden

    Urban Gullberg, Eva Nilsson & Inge Olsson

Authors
  1. Urban Gullberg
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  2. Eva Nilsson
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  3. Inge Olsson
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Editor information

Editors and Affiliations

  1. Physiology Department, Uniformed Services University of the Health Sciences, 20814, Bethesda, MD, USA

    S. J. Baum

  2. Laboratory of Microbiology and Immunology, National Institute of Dental Research, NIH, 29782, Bethesda, MD, USA

    Dov H. Pluznik

  3. Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    L. A. Rozenszajn

  4. Department of Medical Laboratories, Meir Hospital, Kfar-Sava, Israel

    L. A. Rozenszajn

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© 1986 Springer-Verlag New York Inc.

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Gullberg, U., Nilsson, E., Olsson, I. (1986). Properties of a T-Lymphocyte Derived Differentiation Inducing Factor (DIF) for the Myeloid Leukemic Cell Line HL-60. In: Baum, S.J., Pluznik, D.H., Rozenszajn, L.A. (eds) Experimental Hematology Today—1985. Experimental Hematology Today, vol 1985. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4920-7_12

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  • DOI: https://doi.org/10.1007/978-1-4612-4920-7_12

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-0-387-96273-3

  • Online ISBN: 978-1-4612-4920-7

  • eBook Packages: Springer Book Archive

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