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Arrangement of Eukaryotic Initiation Factor 3 and Messenger RNA within Preinitiation Complexes

  • Chapter
Structure, Function, and Genetics of Ribosomes

Part of the book series: Springer Series in Molecular Biology ((SSMOL))

Abstract

Investigations on the structure of initiation complexes of protein biosynthesis are essential prerequisites for understanding the arrangement of the various components and their interactions at the molecular level. Initiation of protein biosynthesis in eukaryotic cells needs at least nine initiation factors (eIF-1, eIF-2, eIF-2B, eIF-3, eIF-4A, eIF-4B, eIF-4C, eIF-4F, and eIF-5) as described by Trachsel et al. (1977), Thomas etal. (1981), and Clemens etal. (1982) (for reviews see Jagus etal., 1981; Maitra etal., 1982). For analysis of the function of the individual initiation factors, different complexes were isolated and characterized. First the ternary complex of eIF-2-GTP-Met-tRNAf becomes bound to a 43S preinitiation complex that contains the small ribosomal subunit, eIF-3, and other factors (Sundkvist and Staehelin, 1975). Thereupon, mRNA is attached, forming a 48S preinitiation complex. After detachment of initiation factors from the 48S complex, the large ribosomal subunit associates, forming an 80S initiation complex.

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Authors
  1. P. Westermann
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  2. R. Benndorf
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  3. G. Lutsch
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  4. H. Bielka
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  5. O. Nygård
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Editors and Affiliations

  1. Clayton Foundation Biochemical Institute Department of Chemistry, The University of Texas at Austin, 78712, Austin, Texas, USA

    Boyd Hardesty  & Gisela Kramer  & 

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Westermann, P., Benndorf, R., Lutsch, G., Bielka, H., Nygård, O. (1986). Arrangement of Eukaryotic Initiation Factor 3 and Messenger RNA within Preinitiation Complexes. In: Hardesty, B., Kramer, G. (eds) Structure, Function, and Genetics of Ribosomes. Springer Series in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4884-2_37

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  • DOI: https://doi.org/10.1007/978-1-4612-4884-2_37

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9346-0

  • Online ISBN: 978-1-4612-4884-2

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