Advertisement

Structural Dynamics of the Translating Ribosome

  • I. N. Serdyuk
  • A. S. Spirin
Part of the Springer Series in Molecular Biology book series (SSMOL)

Abstract

The process of translation (elongation) on the ribosome is composed of repeating cycles, each consisting of three successive steps: aminoacyl-tRNA binding, transpeptidation, and translocation. The question arises: Is any step of the elongation cycle accompanied by mechanical alterations of the ribosomal particle? This question is practically appropriate in considering the translocation step as it includes intraribosomal displacements of a template and the products of the transpeptidation reaction, e.g., the release of deacylated tRNA, the transport of peptidyl-tRNA from one site to the other, and the shift of the template polynucleotide by one codon. The possibility of “an alternate contraction and expansion of the ribosome” (“a pulsating ribosome contraction”) in the elongation cycle was first suggested by Lipmann and co-workers (Conway and Lipmann, 1964; Nishizuka and Lipmann, 1966). This question was posed, most clearly in 1968 when the idea of a type of moving apart (unlocking) of the two coupled ribosomal subunits as a plausible driving mechanism for translocation was proposed (Spirin, 1968).

Keywords

Ribosomal Subunit Elongation Cycle Ribosome Compactness Transpeptidation Reaction Ribosome Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baranov, V.I. (1983). Preparation of translating ribosomes using columns with immobilized polyuridylic acid. Bioorgan. Khimiya (USSR) 9: 1650–1657.Google Scholar
  2. Baranov, V.I., Belitsina, N.V., Spirin, A.S. (1979). The use of columns with matrix- bound polyuridylic acid for isolation of translating ribosomes. In: Nucleic acids Structural Dynamics of the Translating Ribosome and protein synthesis, eds. Moldave, K., Grossman, J. Academic Press, New York/San Francisco/London 59: 382–398.Google Scholar
  3. Conway, T.M., Lipmann, F. (1964). Characterization of a ribosome-linked guano- sine triphosphate in E. coli extracts. Proc. Natl. Acad. Sci. USA 52: 1462–1469.PubMedCrossRefGoogle Scholar
  4. Crichton, R.R., Engelman, D.M., Haas, J., Koch, M.H.J., Moore, P.B., Parfait, R. (1977). Contrast variation study of specifically deuterated E. coli ribosomal subunits. Proc. Natl. Acad. Sci. USA 74: 5547–5550.PubMedCrossRefGoogle Scholar
  5. Glatter, O. (1982). Data treatment. In: Small angle X-ray scattering, eds. Glatter, O., Kratky, O. Academic Press, London, New York, pp. 119–149.Google Scholar
  6. Gudkov, A.T., Gongadze, G.M. (1984). The L7/L12 proteins change their conformation upon interaction of EF-G with ribosomes. FEBS Lett. 176: 32–35.PubMedCrossRefGoogle Scholar
  7. Guinier, A., Fournet, G. (1955). In: Small-angle scattering of X-rays, Wiley, New York/Chapman Hall, London, 148–160.Google Scholar
  8. Ibel, K., Stuhrmann, H.B. (1975). Comparison of neutron and X-ray scattering of dilute myoglobin solutions. J. Mol. Biol. 93:255–265.PubMedCrossRefGoogle Scholar
  9. Jacrot, B. (1976). The study of biological structures by neutron scattering from solution. Rep. Progr. Phys. 39: 911–953.CrossRefGoogle Scholar
  10. Nishizuka, Y., Lipmann, P. (1966). The interrelation between guanosine triphosphatase and amino acid polymerization. Arch. Biochem. Biophys. 116: 344–351.PubMedCrossRefGoogle Scholar
  11. Serdyuk, I.N., Shpungin, J.L., Zaccai, G. (1980). Neutron scattering study of the 13S fragment of 16S RNA and its complex with ribosomal protein S4. J. Mol. Biol. 137: 109–121.PubMedCrossRefGoogle Scholar
  12. Simon, O. (1971). Determination of small alterations in the radius of gyration by small-angle X-ray scattering. J. Appi. Cryst. 4: 317–318.CrossRefGoogle Scholar
  13. Spirin, A.S. (1968). A model of the functioning ribosome: locking and unlocking of ribosome subparticles. Dokl. Akad. Nauk SSSR 179: 1467–1470.PubMedGoogle Scholar
  14. Spirin, A.S. (1969). A model of the functioning ribosome: locking and unlocking of the ribosome subparticles. In: Cold Spring Harbor Symposia on Quantitative Biology, New York 34: 197–207.Google Scholar
  15. Stuhrmann, H.B., Koch, M.H.I., Parfait, R., Haas, I., Ibel, K., Crichton, R.R. (1978). Determination of the distribution of protein and nucleic acid in the 70S ribosomes of E. coli and their 30S subunits by neutron scattering. J. Mol. Biol. 119: 203–212.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • I. N. Serdyuk
  • A. S. Spirin

There are no affiliations available

Personalised recommendations