Structural Dynamics of the Translating Ribosome
The process of translation (elongation) on the ribosome is composed of repeating cycles, each consisting of three successive steps: aminoacyl-tRNA binding, transpeptidation, and translocation. The question arises: Is any step of the elongation cycle accompanied by mechanical alterations of the ribosomal particle? This question is practically appropriate in considering the translocation step as it includes intraribosomal displacements of a template and the products of the transpeptidation reaction, e.g., the release of deacylated tRNA, the transport of peptidyl-tRNA from one site to the other, and the shift of the template polynucleotide by one codon. The possibility of “an alternate contraction and expansion of the ribosome” (“a pulsating ribosome contraction”) in the elongation cycle was first suggested by Lipmann and co-workers (Conway and Lipmann, 1964; Nishizuka and Lipmann, 1966). This question was posed, most clearly in 1968 when the idea of a type of moving apart (unlocking) of the two coupled ribosomal subunits as a plausible driving mechanism for translocation was proposed (Spirin, 1968).
KeywordsCellulose Codon MgCl Disulfide Sine
Unable to display preview. Download preview PDF.
- Baranov, V.I. (1983). Preparation of translating ribosomes using columns with immobilized polyuridylic acid. Bioorgan. Khimiya (USSR) 9: 1650–1657.Google Scholar
- Baranov, V.I., Belitsina, N.V., Spirin, A.S. (1979). The use of columns with matrix- bound polyuridylic acid for isolation of translating ribosomes. In: Nucleic acids Structural Dynamics of the Translating Ribosome and protein synthesis, eds. Moldave, K., Grossman, J. Academic Press, New York/San Francisco/London 59: 382–398.Google Scholar
- Glatter, O. (1982). Data treatment. In: Small angle X-ray scattering, eds. Glatter, O., Kratky, O. Academic Press, London, New York, pp. 119–149.Google Scholar
- Guinier, A., Fournet, G. (1955). In: Small-angle scattering of X-rays, Wiley, New York/Chapman Hall, London, 148–160.Google Scholar
- Spirin, A.S. (1969). A model of the functioning ribosome: locking and unlocking of the ribosome subparticles. In: Cold Spring Harbor Symposia on Quantitative Biology, New York 34: 197–207.Google Scholar