Functional Studies of p24: Platelet Aggregation Inhibition by Fab Monomers of BA-2
The cell surface antigen p24, first detected by monoclonal antibody BA-2 (1), has been found to occur in a variety of cells including both normal and leukemic bone marrow lymphohematopoietic progenitor cells (1), leukemia/lymphoma cell lines (2), activated peripheral blood T cells (3,4), myeloid progenitors, and platelets (4,5). Human and non-human renal cells and platelets (5–6,7), smooth muscle and capillaries (5,7), human foreskin fibroblasts (unpublished data), and a variety of epithelial cells (5) also express this antigen. The widespread distribution of this molecule suggests that it may play a vital role in basic cell physiology. To investigate the role of p24 in normal cell functions, we have performed platelet aggregation studies with both intact BA-2 IgG and BA-2 Fab monomers. Similar studies were performed by Boucheix et al. (8) using monoclonal antibody ALB6 which we have known by blocking studies to be directed against the same or adjacent epitope of p24.
KeywordsPlatelet Aggregation Calcium Ionophore Inhibit Platelet Aggregation Platelet Membrane Human Foreskin Fibroblast
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