Functional Studies of p24: Platelet Aggregation Inhibition by Fab Monomers of BA-2

  • Jo Ellen Brown
  • James G. White
  • R. D. HockettJr.
  • Kathleen R. Hagert
  • John H. Kersey
Conference paper

Abstract

The cell surface antigen p24, first detected by monoclonal antibody BA-2 (1), has been found to occur in a variety of cells including both normal and leukemic bone marrow lymphohematopoietic progenitor cells (1), leukemia/lymphoma cell lines (2), activated peripheral blood T cells (3,4), myeloid progenitors, and platelets (4,5). Human and non-human renal cells and platelets (5–6,7), smooth muscle and capillaries (5,7), human foreskin fibroblasts (unpublished data), and a variety of epithelial cells (5) also express this antigen. The widespread distribution of this molecule suggests that it may play a vital role in basic cell physiology. To investigate the role of p24 in normal cell functions, we have performed platelet aggregation studies with both intact BA-2 IgG and BA-2 Fab monomers. Similar studies were performed by Boucheix et al. (8) using monoclonal antibody ALB6 which we have known by blocking studies to be directed against the same or adjacent epitope of p24.

Keywords

Hydrolysis Toxicity EDTA Leukemia Citrate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kersey, J.H., T.W. LeBien, C.S. Abramson, R. Newman, R. Sutherland, and M. Greaves. 1981. p24: A human leukemia-associated and lymph-hemopoietic progenitor cell surface structure identified with monoclonal antibody. J. Exp. Med. 153: 726.Google Scholar
  2. 2.
    LeBien, T.W., J.H. Kersey, S. Kakazawa, K. Minato, and J. Minowada. 1982. Analysis of human leukemia/lymphoma cell lines with monoclonal antibodies BA-1, BA-2 and BA-3. Leuk. Res. 6: 299.PubMedCrossRefGoogle Scholar
  3. 3.
    Newman, R.A., D.R. Sutherland, T.W. LeBien, J.H. Kersey, and M.F. Greaves. 1982. Biochemical characterization of leukemia associated antigen p24 defined by the monoclonal antibody BA-2. Biochim. Biophys. Acta 701: 318.PubMedCrossRefGoogle Scholar
  4. 4.
    Hercend, T., L.M. Nadler, J.M. Pesando, E.L. Reinherz, S.F. Schlossman, and J. Ritz. 1981. Expression of a 26,000-dalton glycoprotein on activated human T cells. Cell. Immunol. 64: 192.PubMedCrossRefGoogle Scholar
  5. 5.
    Jones, N.H., M.J. Borowitz, and R.S. Metzgar. 1982. Characterization and distribution of a 24,000 dalton antigen defined by a monoclonal antibody (DU- ALL-1) elicited to common acute lymphoblastic leukemia (c-ALL) cells. Leuk. Res. 6: 449.PubMedCrossRefGoogle Scholar
  6. 6.
    Do well, B.L., F.L. Tuck, M.J. Borowitz, T.W. LeBien, and R.S. Metzgar. 1984. Phylogenetic distribution of a 24,000 dalton human leukemia-associated antigen on platelets and kidney cells. Dev. Com. Imm. 8: 187.CrossRefGoogle Scholar
  7. 7.
    Piatt, J.L., T.W. LeBien, and A.F. Michael. 1983. Stages of renal ontogenesis identified by monoclonal antibodies reactive with lymphohemopoietic differentiation antigens. J. Exp. Med. 157: 155.CrossRefGoogle Scholar
  8. 8.
    Boucheix, C., C. Soria, M. Mirshaki, J. Soria, J.-Y. Perrot, M. Fournier, M. Billard, and C. Rosenfeld. 1983. Characteristics of platelet aggregation induced by monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p24): inhibition of aggregation by ALB6 Fab. FEBS Lett. 161: 289.PubMedCrossRefGoogle Scholar
  9. 9.
    Ey, P.L., S.J. Prowse, and C.R. Jenkin. 1978. Isolation of pure IgGl, IgG2a, and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochem. 15: 429.CrossRefGoogle Scholar
  10. 10.
    Brodsky, F.M., P. Parham, C.J. Barnstable, M.J. Crumpton, and W.F. Bod- ner. 1979. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3.PubMedCrossRefGoogle Scholar
  11. 11.
    Porter, R.R. 1959. The hydrolysis of rabbit r-globulin and antibodies with crystalline papain, Biochem. J. 73: 119.PubMedGoogle Scholar
  12. 12.
    Kaye, J., and C.A. Janeway. 1984. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T-cell clone blocks activation by either allogeneic la or antigen and self-Ia. J. Exp. Med. 159: 1397.PubMedCrossRefGoogle Scholar
  13. 13.
    Vercelotti, G., W. Muliins, R. Curry, G. Gaudernack, C. Moldow, and R.P. Messner. 1982. A unique monoclonal antibody inhibits the second wave of platelet aggregation. Clin. Immunol. Immunopathol. 23: 691.PubMedCrossRefGoogle Scholar
  14. 14.
    Curry, R.A., R.P. Messner, and G.J. Johnson. 1984. Inhibition of platelet aggregation by monoclonal antibody reactive with β2-microglobulin chain of HLA complex. Science 224: 509.PubMedCrossRefGoogle Scholar

Copyright information

© Spriger-Verlag New York Inc. 1986

Authors and Affiliations

  • Jo Ellen Brown
  • James G. White
  • R. D. HockettJr.
  • Kathleen R. Hagert
  • John H. Kersey

There are no affiliations available

Personalised recommendations