Changes with in vitro Activation of the B Cell Panel Antigens

  • Arnold S. Freedman
  • Andrew W. Boyd
  • David C. Fisher
  • Stuart F. Schlossman
  • Lee M. Nadler


With exposure to specific antigenic or mitogenic stimuli, B cells proliferate and subsequently differentiate into antibody-secreting cells. The initial events which accompany activation include an increase in cell size, augmented DNA and RNA synthesis, and changes in cell surface structures. The expression of surface immunoglobulin (SIg) and la, the classical surface markers of B cells, has been extensively studied during in vitro stimulation. Upon activation, resting B cells which express IgM and/or IgD will lose SIgD, and then no longer express membrane Ig or switch to the expression of other isotypes (1). Similarly, resting B cells continue to express la after activation until the plasma cell stage (2). In our laboratory, utilizing a panel of monoclonal antibodies directed against B cell differentiation antigens, we have noted changes in the cell surface pheno- type of resting B cells with activation. Two B cell-restricted antigens, B1 and B4, have been observed to be expressed on resting and activated B cells, and lost prior to the terminal stages of differentiation (3,4). The B2 antigen, present on resting cells is lost during the early stages of activation (4,5). We and others have described additional antigens which are not expressed on resting B cells but only appear with activation. These acti-vation antigens include B-LAST 1 (6), B5 (7), interleukin-2 receptor (IL- 2R) (8,9), BB1 (10), 4F2 (11), and the transferrin receptor (11).


Chronic Lymphocytic Leukemia Antigen Expression P140 Antigen Activation Antigen Mantle Zone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Goding, J.W., D.W. Scott, and J.E. Layton. 1977. Genetics, cellular expression and function of IgD and IgM receptors. Immunol. Rev. 37: 152.PubMedCrossRefGoogle Scholar
  2. 2.
    Halper, J.S., S.M. Fu, C.Y. Winchester, and H.G. Kunkel. 1978. Patterns of expression of human la-like antigens during the terminal stage of B cell development. J. Immunol. 120: 1480.PubMedGoogle Scholar
  3. 3.
    Nadler, L.M., K.C. Anderson, G. Marti, M. Bates, E. Park, J.F. Daley, and S.F. Schlossman. 1983. B4, a human B lymphocyte-associated antigen ex-pressed on normal, mitogen-activated, and malignant B lymphocytes. J. Immunol. 131: 244.PubMedGoogle Scholar
  4. 4.
    Boyd, A.W., K.C. Anderson, A.S. Freedman, D.C. Fisher, B. Slaughenhoupt, S.F. Schlossman, and L.M. Nadler, 1985. Studies of in vitro activation and differentiation of human B lymphocytes. I. Phenotypic and functional characterization of the B cell population responding to anti-Ig antibody J. Immunol. 134: 1516.PubMedGoogle Scholar
  5. 5.
    Stashenko, P., L.M. Nadler, R. Hardy, and S.F. Schlossman. 1981. Expression of cell surface markers after human B cell activation. Proc. Natl. Acad. Sci. U.S.A. 78: 3848.PubMedCrossRefGoogle Scholar
  6. 6.
    Thorley-Lawson, D.A., R.T. Schooley, A.K. Bhan, and L.M. Nadler. 1982. Epstein-Barr virus superinduces a new human B cell differentiation antigen (B-LAST 1) expressed on transformed lymphoblasts. Cell 30: 415.PubMedCrossRefGoogle Scholar
  7. 7.
    Freedman, A.S., A.W. Boyd, K.C. Anderson, D.C. Fisher, S.F. Schlossman, and L.M. Nadler. 1985. B5, a new B cell restricted activation antigen. J. Immunol. 134: 2228.PubMedGoogle Scholar
  8. 8.
    Tsudo, M., T. Uchiyama, and H. Uchino. 1984. Expression of TAC antigen on activated normal human B cells. J. Exp. Med. 160: 612.PubMedCrossRefGoogle Scholar
  9. 9.
    Boyd, A.W., D.C. Fisher, D. Fox, S.F. Schlossman, and L.M. Nadler. 1985. Structural and functional characterization of IL-2 receptors on activated B cells. J. Immunol. 134: 2387.PubMedGoogle Scholar
  10. 10.
    Yokochi, T., R.D. Holly, and E.A. Clark. 1982. B lymphoblast antigen (BB-1) expressed on Epstein-Barr virus-activated B cell blasts, B lymphoblastoid cell lines, and Burkitt’s lymphomas. J. Immunol. 128: 823.PubMedGoogle Scholar
  11. 11.
    Kerhl, J.H., A. Muraguchi, and A.S. Fauci. 1984. Differential expression of cell activation markers after stimulation of resting B lymphocytes. J. Immunol. 132: 2857.Google Scholar
  12. 12.
    Reinherz, E.L., and S.F. Schlossman. 1980. The differentiation and functions of human T lymphocytes: a review. Cell 19: 821.PubMedCrossRefGoogle Scholar
  13. 13.
    Stashenko, P., L.M. Nadler, R. Hardy, and S.F. Schlossman. 1980. Characterization of a human B lymphocyte-specific antigen. J. Immunol. 125: 1678.PubMedGoogle Scholar
  14. 14.
    Todd, R.F. III, L.M. Nadler, and S.F. Schlossman. 1981. Antigens on human monocytes identified by monoclonal antibodies. J. Immunol. 126: 1435.PubMedGoogle Scholar
  15. 15.
    Meuer, S.C., R.E. Hussey, M. Fabbi, D. Fox, O. Acuto, K.A. Fitzgerald, J.C. Hodgdon, J.P. Protentis, S.F. Schlossman, and E.L. Reinherz. 1984. An alternate pathway of T cell activation: A functional role for the 550Kd Til sheep erythrocyte receptor protein. Cell 36: 897.PubMedCrossRefGoogle Scholar
  16. 16.
    Abramson, C.S., J.H. Kersey, and T.W. LeBien. 1981. A monoclonal anti-body (BA-1) reactive with cells of human B lymphocyte lineage. J. Immunol. 126: 83.PubMedGoogle Scholar
  17. 17.
    Omary, M.B., I.S. Trowbridge, and H.A. Battifora. 1980. Human homologue of murine T200 glycoprotein. J. Exp. Med. 152: 842.PubMedCrossRefGoogle Scholar
  18. 18.
    Nadler, L.M., P. Stashenko, R. Hardy, J.M. Pesando, E.J. Yunis, and S.F. Schlossman. 1981. Monoclonal antibodies defining serologically distinct HLA-D/DR related la-like antigen in man. Hum. Immunol. 1: 77.CrossRefGoogle Scholar
  19. 19.
    Nadler, L.M., P. Stashenko, R. Hardy, A. van Agthoven, C. Terhorst, and S.F. Schlossman. 1981. Characterization of a human B cell-specific antigen (B2) distinct from Bl. J. Immunol. 125: 1941.Google Scholar
  20. 20.
    Bhan, A.K., L.M. Nadler, P. Stashenko, and S.F. Schlossman. 1981. Stages of B cell differentiation in human lymphoid tissues. J. Exp. Med. 154: 737.PubMedCrossRefGoogle Scholar
  21. 21.
    Hsu, S., and E.S. Jaffe. 1984. Phenotypic expression of B lymphocytes. 1. Identification with monoclonal antibodies in normal lymphoid tissues. Am. J. Pathol. 114: 387.PubMedGoogle Scholar
  22. 22.
    Anderson, K.C., M.P. Bates, B.L. Slaughenhoupt, G.S. Pinkus, S.F. Schlossman, and L.M. Nadler. 1984. Expression of human B cell-associated antigens on leukemias and lymphomas: A model of human B cell differentiation. Blood 63: 1424.PubMedGoogle Scholar
  23. 23.
    Iida, K., L.M. Nadler, and V. Nussenzweig. 1983. Identification of the membrane receptor for the complement fragment C3d by means of a monoclonal antibody. J. Exp. Med. 158: 1021.PubMedCrossRefGoogle Scholar
  24. 24.
    Fingeroth, J.D., J.J. Weis, T.F. Tedder, J.L. Strominger, P.A. Biro, and D.T. Fearon. 1984. Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2. Proc. Natl. Acad. Sci. U.S.A. 81: 4510.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Arnold S. Freedman
  • Andrew W. Boyd
  • David C. Fisher
  • Stuart F. Schlossman
  • Lee M. Nadler

There are no affiliations available

Personalised recommendations