Quantitative Phenotypes of B Chronic Lymphocytic Leukemia B Cells Established with Monoclonal Antibodies from the B Cell Protocol
As seen at the First International Workshop on Human Leucocyte Differentiation Antigens (1), comparison of the serological specificities of mAbs is a very cumbersome task which requires testing reagents on a very broad panel of cell types. Flow microfluorimetry (FMF) analysis was used as well as fluorescence microscopy to perform such studies and finally each sample was characterized, for the purpose of statistical comparisons, by a single figure (2). Indeed, FMF-derived histograms are very rich pieces of data, which are only partly summarized by indicating the percentage of reactive cells. In order to get as much significant information as possible from a limited panel of cells, we propose the use of an additional numerical parameter, the mean antigen density of the cell (sub)population(s), obtained by absolute quantitative measurements of the mean amount of mAb molecules bound to the selected cells under saturating conditions.
KeywordsLymphoma Leukemia Fluores Tated Paraformaldehyde
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