Early in this symposium, distinguished scientists summarized the experimental evidence for the orderly organization of the genetic material within mammalian cells, from the primary (base sequences, as discussed by Baltimore, Watson, and Temin, in reference to gene structure and expression) to the secondary (the various forms of the DNA double helix discussed by Rich) and tertiary (the wrapping of DNA around octamer histones to form the nucleosome, as discussed by Bradbury) structures. Large uncertainty—because of the inherent difficulties of an in situ characterization—still exists as to the exact three-dimensional geometry of the higher-order DNA foldings (from the supernucleosomes on); suggestions and evidences for the quaternary and higher-order structures are hereby summarized, along with other nuclear structures, with a possible clue to the higher-order gene structures.
KeywordsSucrose Magnesium Enthalpy Bromide Titration
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