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Neutron Scatter and Diffraction Techniques Applied to Nucleosome and Chromatin Structure

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Bioscience at the Physical Science Frontier

Abstract

The application of neutron scatter and diffraction techniques to structural studies of the nucleosome and of chromatin provides a striking example of the interface of modern physics and the life sciences. The structure and function of the eukaryotic chromosome is a major problem in molecular cell biology. One approach toward an understanding of the functions of such a complex system is to determine (i) the structure of the repeating chromatin subunit, the nucleosome, (ii) the arrangements of nucleosomes in the different orders of chromatin structure, and (iii) how these structural parameters change in response to the functional requirements of cells, i.e., to approach function through an understanding of structure.

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References

  1. Compton, J. L., Bellard, M., and Chambon, P. (1976), Proc. Natl. Acad. Sci. USA 73, 4382.

    Article  PubMed  CAS  Google Scholar 

  2. Stein, A., and Binou, M. (1984), J. Mol. Biol. 178, 341.

    Article  PubMed  CAS  Google Scholar 

  3. Bradbury, E. M., and Matthews, H. R. (1980), Cell Growth, in NATO Advanced Study Institutes Series (Nicolini, C., ed.) Plenum, NY, vol. 38, pp. 411–454.

    Google Scholar 

  4. Allfrey, V. G. (1976), Chromatin and Chromosome Structure (Li, H. J., Eckhardt, R., eds.) Academic, FL, pp. 167–191.

    Google Scholar 

  5. Goldknopf, I. L., Taylor, C. W., Baum, R. W., Yeoman, L. C. Olsen, M. O. J., Prestayko, A. W., and Busch, M. (1975), J. Biol. Chem. 250, 7182.

    PubMed  CAS  Google Scholar 

  6. West, M. H. P., and Bonner, W. M. (1980), Nucleic Acids. Res. 8, 4671.

    Article  PubMed  CAS  Google Scholar 

  7. Wu. R. S., Kohn, K. W., and Bonner, W. M. (1981), J. Biol. Chem. 256, 5916.

    PubMed  CAS  Google Scholar 

  8. Langan, T. A. (1978), Methods in Cell Biology (Stein, G. S., and Stein, J., eds.), Academic, FL, vol. 19, pp. 127–142.

    Google Scholar 

  9. Gurley, L. R., Tobey, R. A., Walters, P. A., Hilderbrand, C. E., Hohman, P. G., D’Anna, J. A., Barham, S. S., and Deavan, L. L. (1978), Cell Cycle Regulation (Jeter, J. R., Cameron, I. L., Padilla, G. M., and Zimmerman, R. M., eds.) Academic, FL, pp. 37–60.

    Google Scholar 

  10. Levinger, L., and Varshavsky, A. (1982), Cell 28, 375.

    Article  PubMed  CAS  Google Scholar 

  11. Mueller, R. D., Yasuda, H., Hatch, C. L., Bonner, W. M., and Bradbury, E. M. (1985), J. Biol. Chem. 260, 5147.

    PubMed  CAS  Google Scholar 

  12. Weisbrod, S., and Weintraub, H. (1979), Proc. Natl. Acad. Sci. USA 76, 630.

    Article  PubMed  CAS  Google Scholar 

  13. Weisbrod, S., Groudine, M., and Weintraub, H. (1980), Cell 19, 289.

    Article  PubMed  CAS  Google Scholar 

  14. Kneale, G. G., Baldwin, J. P., and Bradbury, E. M. (1977), Q. Rev. Biophys. 10, 485.

    Article  PubMed  CAS  Google Scholar 

  15. Stuhrmann, H. B. (1974), J. Appi. Cryst. 7, 173.

    Article  CAS  Google Scholar 

  16. Stuhrmann, H. B. (1975), Brookhaven Symposia in Biology 27, 3–19.

    Google Scholar 

  17. Ibel, K., and Stuhrmann, H. B. (1975), J. Mol. Biol. 93, 255.

    Article  PubMed  CAS  Google Scholar 

  18. Moore, P. B., Langer, J. A., Schoenborn, B. P., and Engelman, D. M. (1977), J. Mol. Biol. 112, 199.

    Article  PubMed  CAS  Google Scholar 

  19. Engelman, D. M. (1979), Methods in Enzymology, vol. 59, ( Moldave, K., and Grossman, L., eds.), Academic, FL, pp. 656–669.

    Google Scholar 

  20. Bradbury, E. M., Baldwin, J. P., Carpenter, B. G., Hjelm, R. P., Hancock, R, and Ibel, K. (1975), Brookhaven Symp. Biol. 27, 97.

    Google Scholar 

  21. Pardon, J. F., Worcester, D. C., Wooley, J. C., Tatchell, K., van Holde, K. E., and Richards, B. M. (1975), Nucleic Acids Res. 2, 2163.

    Article  PubMed  CAS  Google Scholar 

  22. Hjelm, R. P., Kneale, G. G., Suau, P., Baldwin, J. P., Bradbury, E. M., and Ibel, K. (1977), Cell 10, 139.

    Article  PubMed  CAS  Google Scholar 

  23. Suau, P., Kneale, G. G., Braddock, G. W., Baldwin, J. P., and Bradbury, E. M. (1977), Nucleic Acids Res. 4, 3769.

    Article  PubMed  CAS  Google Scholar 

  24. Finch, J. T., Lutter, L. C., Rhodes, D., Brown, R. S., Rushton, B., Levitt, M., and Klug, A. (1977), Nature 269, 29.

    Article  PubMed  CAS  Google Scholar 

  25. Finch, J. T., Brown, R. S., Rhodes, D., Richmond, T. J., Rushton, B., Lutter, L. C., and Klug, A. (1981), J. Mol. Biol. 145, 757.

    Article  PubMed  CAS  Google Scholar 

  26. Bentley, C. A., Finch, J. T., and Lewit-Bentley, A. (1981), J. Mol. Biol. 145, 771.

    Article  PubMed  CAS  Google Scholar 

  27. Richmond, T. J., Klug, A., Finch, J. T., and Lutter, L. C. (1982), Proc. 2nd SUNYA Conversations, (Sarma, R. H., ed.), Academic, FL, vol. 11, pp. 109–123.

    Google Scholar 

  28. Guinier, A., and Fournet, G. (1955), Small Angle Scattering of X-rays, Wiley and Sons, New York.

    Google Scholar 

  29. Sibbet, G., Carpenter, B. G., Ibel, K., Kneale, G. G., Bradbury, E. M., and Baldwin, J. P. (1983), Eur. J. Biochem. 133, 393.

    Article  PubMed  CAS  Google Scholar 

  30. Braddock, G. W., Baldwin, J. P., and Baldwin, E. M. (1981), Biopolymers 20, 327.

    Article  PubMed  CAS  Google Scholar 

  31. Cary, P. D., Moss, T., and Bradbury, E. M. (1979), Eur. J. Biochem. 89, 475.

    Article  Google Scholar 

  32. Simpson, R. T. (1978), Biochemistry 17, 5524.

    Article  PubMed  CAS  Google Scholar 

  33. Thoma, F., Koller, Th., and Klug, A. (1979), J. Cell. Biol. 83, 403.

    Article  PubMed  CAS  Google Scholar 

  34. Chapman, G. E., Hartman, P. G., and Bradbury, E. M. (1976), Eur. J. Biochem. 61, 69.

    Article  PubMed  CAS  Google Scholar 

  35. Hartman, P. G., Chapman, G. E., Moss, T., and Bradbury, E. M. (1977), Eur. J. Biochem. 77, 45.

    Article  PubMed  CAS  Google Scholar 

  36. Chapman, G. E., Aviles, F. X., Crane-Robinson, C., and Bradbury, E. M. (1978), Eur. J. Biochem. 90, 287.

    Article  PubMed  CAS  Google Scholar 

  37. Cary, P. D., Hines, M. L., Bradbury, E. M., Smith, B. J., and Johns, E. W. (1981), Eur. J. Biochem. 120, 371.

    Article  PubMed  CAS  Google Scholar 

  38. Crane-Robinson, C., Bohm, L., Puigdomenech, P., Cary, P. D., Hartman, P. G., and Bradbury, E. M., (1980), FEBS DNA-Recombination Interaction and Repair, Pergamon, Oxford and NY, pp. 293–300.

    Google Scholar 

  39. Allan,J., Hartman, P. G., Crane-Robinson, C., and Aviles, F. X. (1980), Nature 288, 675.

    Article  PubMed  Google Scholar 

  40. Laemmli, U. K., Cheng, S. M., Adolph, K. W., Paulson, J. R., Brown, J. A., and Baumbach, W. R. (1978), Cold Spring Harbor Symp. Q. Biol. 42, 351.

    CAS  Google Scholar 

  41. Igo-Kemenes, T., Greil, W., and Zachau,H. G. (1977), Nucleic Acids Res. 4, 3387.

    Article  PubMed  CAS  Google Scholar 

  42. Igo-Kemenes, T., and Zachau, H. G. (1978), Cold Spring Harbor Symp. Q. Biol 42, 109.

    CAS  Google Scholar 

  43. Ris, H., and Kubai, D. F. (1970), Annu. Rev. Genet. 4, 263.

    Article  PubMed  CAS  Google Scholar 

  44. Woodcock, C. L. F. (1973), J. Cell Biol. 59, 737a.

    Google Scholar 

  45. Olins, A. L., and Olins, D. E. (1974), Science 183, 330.

    Article  PubMed  CAS  Google Scholar 

  46. Carpenter, B. G., Baldwin, J. P., Bradbury, E. M., and Ibel, K. (1976), Nucleic Acids Res. 3, 1739.

    PubMed  CAS  Google Scholar 

  47. Finch, J. T., and Klug, A. (1976), Proc. Natl. Acad. Sci. USA 73, 1897.

    Article  PubMed  CAS  Google Scholar 

  48. Suau, P., Bradbury, E. M., and Baldwin, J. P. (1979), Eur. J. Biochem. 97, 593.

    Article  PubMed  CAS  Google Scholar 

  49. Baldwin, J. P., Dunn, S. P., Rattle, H. W. E., Staynov, D. Z., Boulter, E. M., and Carpenter, B. G. (1984), Personal communication.

    Google Scholar 

  50. McGhee, J. D., Nicol, J. M. Felsenfeld, G., and Rau, D. C. (1983), Cell 33, 831.

    Article  PubMed  CAS  Google Scholar 

  51. Mitra, S., Sen, D., and Crothers, D. M. (1983), Nature 308, 247.

    Article  Google Scholar 

  52. Pardon, J. F., and Wilkins, M. H. F. (1972), J. Mol. Biol 68, 115.

    Article  PubMed  CAS  Google Scholar 

  53. Baldwin, J. P., Boseley, P. G., Bradbury, E. M., and Ibel, K. (1975), Nature 253, 245.

    Article  PubMed  CAS  Google Scholar 

  54. Baldwin, J. P., Braddock, G. W., Carpenter, B. G., Kneale, G. G., Simpson, G. K., Suau, P., Hjelm, R. P., and Bradbury, E. M. (1978), J. Appl Cryst. 11, 483.

    Article  Google Scholar 

  55. Baldwin, J. P., Carpenter, B. G., Crespi, H., Hancock, R., Stephens, R. M., Simpson, J. K., Bradbury, E. M., and Ibel, K. (1978), J. Appl Cryst. 11, 484.

    Article  CAS  Google Scholar 

  56. Bradbury, E. M. (1982), Non-Histone Chromosmal Proteins:HMG Proteins, ( Johns, E. W., ed.) Academic, NY, pp. 89–110.

    Google Scholar 

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Bradbury, E.M., Baldwin, J.P. (1986). Neutron Scatter and Diffraction Techniques Applied to Nucleosome and Chromatin Structure. In: Nicolini, C. (eds) Bioscience at the Physical Science Frontier. Humana Press. https://doi.org/10.1007/978-1-4612-4834-7_4

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  • DOI: https://doi.org/10.1007/978-1-4612-4834-7_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9182-4

  • Online ISBN: 978-1-4612-4834-7

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