Intrasplenic Immunization for the Production of Monoclonal Antibodies

  • M. Spitz
  • Lidia Spitz
Part of the Contemporary Biomedicine book series (CB, volume 7)


With the advent of the monoclonal antibody (Mab) technology, the era of antibodies against antigens gave way to the new concept of antibodies against epitopes or antigenic sites.


Spleen Cell Antigenic Site Fusion Experiment Hybrid Cell Line Immunization Procedure 
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  1. 1.
    Benacerraf B.: 1978. A hypothesis to relate the specificity of T lymphocytes and the activity of I region-specific Ir. Genes in macrophages and B lymphocytes. J. Immunol. 120: 1809–1812.PubMedGoogle Scholar
  2. 2.
    Stahli C., Staehelin T., Miggiano W., Schmidt J., and Haring P.: 1980. High frequencies of antigen-specific hybridomas: Dependence on immunization paramters and prediction by spleen cell analysis. J. Immunol. Meth. 32: 297–304.CrossRefGoogle Scholar
  3. 3.
    Anderson J. and Melchers F.: 1978. The antibody repertoire of hybrid cell lines obtained by fusion of X63-AG8 myeloma cells with mitogen activated B-cell blasts. Curr. Top. Microbiol. Immunol. 81: 130–139.Google Scholar
  4. 4.
    Oi V. T., Jones P. P., Herzemberg L. A., and Herzemberg L. A.: 1978. Properties of monoclonal antibodies to mouse Ig allotypes, H-2 and Ia antigens. Curr. Top. Microbiol. Immunol. 81: 115–129.PubMedGoogle Scholar
  5. 5.
    Spitz M., Spitz L., Thorpe R., and Eugui E: 1984. Intrasplenic primary immunization for the production of monoclonal antibodies. J. Immunol. Meth. 70: 39–43.CrossRefGoogle Scholar
  6. 6.
    Galfre J., Howe S. C, Milstein C.: 1977. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266: 550–552.PubMedCrossRefGoogle Scholar
  7. 7.
    Siskind G. W. and Benaccerraf B.: 1969. Cell selection by antigen in the immune response. Adv. Immunol. 10: 1–50.PubMedCrossRefGoogle Scholar
  8. 8.
    Lanzavechia A.: 1985. Antigen-specific interaction between T and B cells. Nature 314: 537–539.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • M. Spitz
  • Lidia Spitz

There are no affiliations available

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